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  1. Pub 'PMID:22595514'

Title

Initial deployment of the cardiogenic gene regulatory network in the basal chordate, Ciona intestinalis.

Authors

Woznica A, Haeussler M, Starobinska E, Jemmett J, Li Y, Mount D, Davidson B

Journal

Dev Biol 2012; 368(1):127-39

PubMed ID

PMID:22595514

Abstract

The complex, partially redundant gene regulatory architecture underlying vertebrate heart formation has been difficult to characterize. Here, we dissect the primary cardiac gene regulatory network in the invertebrate chordate, Ciona intestinalis. The Ciona heart progenitor lineage is first specified by Fibroblast Growth Factor/Map Kinase (FGF/MapK) activation of the transcription factor Ets1/2 (Ets). Through microarray analysis of sorted heart progenitor cells, we identified the complete set of primary genes upregulated by FGF/Ets shortly after heart progenitor emergence. Combinatorial sequence analysis of these co-regulated genes generated a hypothetical regulatory code consisting of Ets binding sites associated with a specific co-motif, ATTA. Through extensive reporter analysis, we confirmed the functional importance of the ATTA co-motif in primary heart progenitor gene regulation. We then used the Ets/ATTA combination motif to successfully predict a number of additional heart progenitor gene regulatory elements, including an intronic element driving expression of the core conserved cardiac transcription factor, GATAa. This work significantly advances our understanding of the Ciona heart gene network. Furthermore, this work has begun to elucidate the precise regulatory architecture underlying the conserved, primary role of FGF/Ets in chordate heart lineage specification.

Data related to this article

Fates affected

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Gene involved Fates

Genes functionally analyzed

1 result

Molecular Tools

1 result

Molecular Tool Name
Mesp>Ets1/2-VP16 (Davidson 2006)

Cis-regulatory regions

No result

Cis-reg Name

Constructs

No result

Construct Name Experimental evidence

RNA-Seq data

No result

Studied Transcriptome Experiment ID