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  1. Pub 'PMID:21303852'

Title

Neuronal subtype specification in the spinal cord of a protovertebrate.

Authors

Stolfi A, Levine M

Journal

Development 2011; 138(5):995-1004

PubMed ID

PMID:21303852

Abstract

The visceral ganglion (VG) comprises the basic motor pool of the swimming ascidian tadpole and has been proposed to be homologous to the spinal cord of vertebrates. Here, we use cis-regulatory modules, or enhancers, from transcription factor genes expressed in single VG neuronal precursors to label and identify morphologically distinct moto- and interneuron subtypes in the Ciona intestinalis tadpole larva. We also show that the transcription factor complement present in each differentiating neuron correlates with its unique morphology. Forced expression of putative interneuron markers Dmbx and Vsx results in ectopic interneuron-like cells at the expense of motoneurons. Furthermore, by perturbing upstream signaling events, we can change the transcription factor expression profile and subsequent identity of the different precursors. Perturbation of FGF signaling transforms the entire VG into Vsx+/Pitx+ putative cholinergic interneurons, while perturbation of Notch signaling results in duplication of Dmbx+ decussating interneurons. These experiments demonstrate the connection between transcriptional regulation and the neuronal subtype diversity underlying swimming behavior in a simple chordate.

Data related to this article

Fates affected

No result

Gene involved Fates

Genes functionally analyzed

No result

Gene Name Experimental evidence

Genes with description of Wild Type Expression

No result

Gene Name Experimental evidence

Constructs

26 results

Construct Name Experimental evidence
p.Cirobu.REG.KH2012.L152:99298-102536>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC5.2824518-2829361>NLS::LacZ Show experimental evidence
p.Cirobu.REG.KhC5.2824518-2829361>H2B::mCherry Show experimental evidence
p.Cirobu.REG.KhC1.9789507-9792995>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KH2012.L24:33143-35824>NLS::LacZ Show experimental evidence
p.Cirobu.REG.KH2012.L24:33143-35824>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KH2012.L152:99298-102536>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KH2012.L152:99298-102536>unc76::CFP Show experimental evidence
p.Cirobu.REG.KhC5.2824518-2829361>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KhC5.2824518-2829361>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC1.9789507-9790838>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KhC1.9789507-9790838>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC1.9789507-9790838>unc76::CFP Show experimental evidence
p.Cirobu.REG.KhC5:42519-45326>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC5:42519-45326>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KhC5:42519-45326>unc76::YFP Show experimental evidence
p.Cirobu.REG.KhC6:403003-407293>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhL153:204692-208149>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KH2012.L24:33143-35824>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC11:4100254-4102604>unc76::mCherry Show experimental evidence
p.Cirobu.REG.KhC11:4100254-4102604>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC11:4100254-4102604>VenusYFP Show experimental evidence
p.Cirobu.REG.KhC11:4100672-4100951>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC11:4100254-4102439>unc76::GFP Show experimental evidence
p.Cirobu.REG.KhC11:4100254-4102439>nls:LacZ Show experimental evidence
pCi-Pitx -7865bp/140bp>VenusYFP Show experimental evidence

RNA-Seq data

No result

Studied Transcriptome Experiment ID