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  1. Pub 'PMID:17624550'
  2. Pub 'PMID:23302037'

Title

Identification of a retinoic acid-responsive neural enhancer in the Ciona intestinalis Hox1 gene.

Authors

Kanda M, Ikeda T, Fujiwara S

Journal

Dev. Growth Differ. 2013; 55(2):260-9

PubMed ID

23302037

Abstract

The Hox1 gene in the urochordate ascidian Ciona intestinalis (Ci-Hox1) is expressed in the nerve cord and epidermis. We identified a nerve cord enhancer in the second intron of Ci-Hox1, and demonstrated that retinoic acid (RA) plays a major role in activating this enhancer. The enhancer contained a putative retinoic acid-response element (RARE). Mutation of the RARE in the Ci-Hox1 nerve cord enhancer only partially abolished the enhancer activity. Genes encoding RA synthase and the RA receptor were knocked down using specific antisense morpholino oligos (MOs), and injection of embryos with these MOs resulted in the complete disappearance of epidermal expression of Ci-Hox1 and reduction of neural expression. However, nerve cord expression was not completely repressed. These results suggest that the nerve cord enhancer is activated by two partially redundant pathways; one RA-dependent and one RA-independent.

Data related to this article

Fates affected

No result

Fates Genes involved

Genes functionally analyzed

No result

Gene Name Experimental evidence

Genes whose regulation was studied

1 result

Genes with description of Wild Type Expression

No result

Gene Name Experimental evidence

Molecular Tools

1 result

Molecular Tool Name
Ci-RAR MO

RNA-Seq data

No result

Studied Transcriptome Experiment ID