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  1. Pub 'PMID:23073965'

Title

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element.

Authors

Hozumi A, Mita K, Miskey C, Mates L, Izsvak Z, Ivics Z, Satake H, Sasakura Y

Journal

Dev. Dyn. 2013; 242(1):30-43

PubMed ID

PMID:23073965

Abstract

Transposon-mediated transgenesis is an excellent method for creating stable transgenic lines and insertional mutants. In the chordate Ciona intestinalis, Minos is the only transposon that has been used as the tool for germline transformation. Adding another transposon system in this organism enables us to conduct genetic techniques which can only be realized with the use of two transposons.

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