Name

Ci-Bra>Tbx2/3-DBD::GFP (José-Edwards 2013)

Targeted Gene

(TBX2; TBX3; TBX4)

Family

Sequence-based

Type

overexpression_construct

Supplier

Comment

The Brachyury promoter is used to misexpress a dominant negative form of Tbx2/3 in notochord cells and GFP (to allow for the detection of incorporation of the transgenes). The Ci-Tbx2/3 DBD was amplified from cDNA employing the primers: 5′-AACCATGGATAACATGGAACTGTGGGAGC-3′ and 5′-CTACTAGTTCCGCTCCCTGAGTCTCG-3′

Regulation

Loss of function

Publication

José-Edwards et al. (2013). Tbx2/3 is an essential mediator within the Brachyury gene network during Ciona notochord development. Development 140(11):2422-33