Name

FoxF[TVC]>Islet::WRPW (Stolfi 2010)

Targeted Gene

(ISL1; ISL2; LHX9)

Family

Sequence-based

Type

overexpression_construct

Supplier

Comment

Construct used to overexpress a repressor version of Islet in the TVC lineage using FoxF enhancer. A region encompassing putative Islet LIM, homeo-, and LBD (Lhx3-binding) domains was amplified from Islet coding sequence by the NheI Islet LIM N fwd (tatgctagctCTATGCGTAGGATGTGGC) and NgoMIV Islet LBD C rev (tatgccggcTTCCAAGCTGGTTGCTGG) primers. It was cloned in frame between an N-terminal HA tag and a WRPW motif, downstream of FoxF-TVC+bpFOG.

Regulation

Loss of function

Publication

Stolfi et al. (2010). Early chordate origins of the vertebrate second heart field. Science 329(5991):565-8