Name

Hr-PEM MO2 (Negishi 2007)

Targeted Gene

(Harore.CG.MTP2014.S480.g14149)

Family

Sequence-based

Type

morpholino_oligo

Supplier

Comment

This morpholino oligo covers the starting methionine codon.

Regulation

Loss of function

Publication

Kumano et al. (2011). A maternal factor unique to ascidians silences the germline via binding to P-TEFb and RNAP II regulation. Curr. Biol. 21(15):1308-13

Kumano et al. (2009). Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localized PEM. Dev. Biol. 331(1):78-88

Kumano et al. (2010). Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant. Dev Biol 344(1):284-92

Negishi et al. (2007). Localized PEM mRNA and protein are involved in cleavage-plane orientation and unequal cell divisions in ascidians. Curr Biol 17(12):1014-25

Miyaoku et al. (2018). Control of Pem protein level by localized maternal factors for transcriptional regulation in the germline of the ascidian, Halocynthia roretzi. PLoS One 13(4):e0196500

Zheng et al. (2020). H3K27me3 suppresses sister-lineage somatic gene expression in late embryonic germline cells of the ascidian, Halocynthia roretzi. Dev Biol 460(2):200-214