Name

Coe>Dmbx (Stolfi 2011)

Targeted Gene

(DMBX1; DRGX; PITX3)

Family

Sequence-based

Type

overexpression_construct

Supplier

Comment

The 2.6 kb genomic DNA segment located upstream of Coe (REG00001266) directs expression of Dmbx (C1.1212) in all of the differentiating visceral ganglion neural precursors. Dmbx protein-coding cDNA sequence was amplified by RT-PCR. First-strand cDNA synthesis was performed off mixed-stage embryo whole mRNA preparations using oligo-dT primer. For PCR, the following primers were used (left to right=5′ to 3′): Dmbx cDNA N F, ATGAATTATTATGACGCAATTCGTGC; Dmbx cDNA C R, CGATTACATCATCATTTCTTGGATTG; Dmbx primers were designed based on available mRNA, EST and genomic sequence data.

Regulation

Gain of function

Publication

Stolfi et al. (2011). Neuronal subtype specification in the spinal cord of a protovertebrate. Development 138(5):995-1004