Name

Su(H)DBM RNA (Hudson 2006)

Targeted Gene

(RBPJ; RBPJL)

Family

Sequence-based

Type

mRNA

Supplier

Comment

mRNA encoding a dominant negative form of Suppressor of Hairless, a transcription factor known to mediate Notch signalling.

Ci-Su(H)DBM was generated by introducing mutations R218E, R220E, R227E and Y228S into the DNA-binding domain of Ci-Su(H) by overlap- extension PCR. Mutations at these four sites result in a DNA-binding mutant that acts in a dominant-negative manner

Regulation

Loss of function

Publication

Hudson et al. (2006). A signalling relay involving Nodal and Delta ligands acts during secondary notochord induction in Ciona embryos. Development 133(15):2855-64

Esposito et al. (2017). Patterning of brain precursors in ascidian embryos. Development 144(2):258-264