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MOLECULAR TOOL

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  1. Molecular Tool 'Tbx6.b MO (Tokuoka 2018)'

Molecular tool card for 'Tbx6.b MO (Tokuoka 2018)'

Name

Tbx6.b MO (Tokuoka 2018)

Targeted Gene

(TBX2; TBX3; TBX6)

Family

Sequence-based

Type

morpholino_oligo

Supplier

Comment

Morpholino oligo against Tbx6.b (S654.1-3).
Authors' comment : Given that Tbx6.b is a multicopy gene, we injected a mixture of the following two MOs so that all copies were knocked down, as reported previously (Yagi et al., 2005): 5′-TTGAGCCTCTCACGTCTGTCGCCAT-3′and 5′-TTACAATTTCCTCTCTCTTTCGATT-3′. MOs were injected by microinjection under amicroscope,

Regulation

Loss of function

Sequence

TTGAGCCTCTCACGTCTGTCGCCAT/TTACAATTTCCTCTCTCTTTCGATT