Name

pSoxB2>Nkx-c

Targeted Gene

(BARHL1; BARHL2; TLX1)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

“We have used electroporation-mediated overexpression using an independent ectodermal drivers. The promoter of SoxB2 drives transcription in the posterior ectoderm lineage, b-line, with the exclusion of the b6.5 lineage (dorsal midline precursors) from the 64-cell stage. This driver allow assessing at the same time ectopic gene activation in the flanks of the tail epidermis and gene inhibition at the midlines.”

Regulation

Gain of function

Publication

Roure et al. (2016). Msxb is a core component of the genetic circuitry specifying the dorsal and ventral neurogenic midlines in the ascidian embryo. Dev. Biol. 409(1):277-287