Name

pETR>Snail

Targeted Gene

(SNAI1; SNAI2; SNAI3)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

The upstream regulatory sequences of ETR drives Snail expression throughout the a- and A-line neural cells from early gastrula stages.

Materials and methods: pETR>Snail was generated as follows: The ETR upstream sequences were PCR-amplified from genomic DNA of Ciona intestinalis using the following primers (ETR-up-attB3- F3 ATAAAGTAGGCTCCGTCACGTGATCCCGTTTCC, ETR-up-attB5-R3 GAAAAGTTGGGTGTCGTTTATCCCCGCTTTGGC) and subcloned into pDONR 221 P3-P5 (Roure et al., 2007) to generate pENTR-L3-ETR-L5. The ORF of Snail was PCR-amplified from the cDNA (Corbo et al., 1997) using the following primers (Snail-F-attB1 AAAAAGCAGGC- TACCATGACCTCCGTCGAGCCCATG, Snail-R-attB2 AGAAAGCTGGGTT- TAGGATGCTGTCTTGCGTTGTGC) and subcloned into pDONR 221 P1–P2 to generate pENTR-L1-Snail-L2. These entry clones were mixed with pSP1.72BSSPE-R3-ccdB/cmR–R5::RfA (Roure et al., 2007) in a LR reaction to generate pETR4Snail.

Regulation

Gain of function

Publication

Hudson et al. (2015). Snail mediates medial-lateral patterning of the ascidian neural plate. Dev. Biol. 403(2):172-9

Esposito et al. (2017). Patterning of brain precursors in ascidian embryos. Development 144(2):258-264