Name

pBra>Neurogenin

Targeted Gene

(NEUROG1; NEUROG2; NEUROG3)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

This construct overexpresses Neurogenin in most of the notochord cells. The pBra–Neurogenin construct was prepared by excising the Neurogenin CDS contained in the pCRsII vector through digestion with BamHI and MluI restriction enzymes, and cloning it in the pBS/pBra700/SV40 construct previously digested with the same enzymes.

Regulation

Gain of function

Publication

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87