Name

Mesp>Ets1/2-WRPW (Davidson 2006)

Targeted Gene

(ETS1; ETS2; ETV1)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

Construct used to overexpress a repressor version of Ets1/2 in the B7.5 lineage using Mesp enhancer. The Ets1/2 DNA-binding domain was amplified from an Ets1/2EST clone (R1CiGC01h16) obtained from the Ciona intestinalis Gene Collection Release 1 (Satou et al. 2005) using the follow-ing primers (lower case indicates restriction site and flanking base pairs): EtsDBDf, aaagctagcGCCTATACTGACACCTTTGAAGCC ; EtsDBDr, aaaactagtATCATCACTTAGTCGATCTTCAGGC. It was then fused in-frame into modified pCES vectors containing the full-length Mesp enhancer and repressor (WRPW) domains (modified from Mesp–MespVp16) (Davidson et al. 2005). The WRPW domain consists of the following sequence replacing Vp16 by ligation at the Nhe1 and Spe1 sites in Mesp–EtsVp16: CAGATCAAGGAGGAGGAGCAGCCCTGGCGGCCCTGGTAATAA

Regulation

Loss of function

Publication

Stolfi et al. (2014). Divergent mechanisms regulate conserved cardiopharyngeal development and gene expression in distantly related ascidians. Elife 3():e03728

Stolfi et al. (2010). Early chordate origins of the vertebrate second heart field. Science 329(5991):565-8

Davidson et al. (2006). FGF signaling delineates the cardiac progenitor field in the simple chordate, Ciona intestinalis. Genes Dev. 20(19):2728-38