Name

Mesp>Ets1/2-VP16 (Davidson 2006)

Targeted Gene

(ERG; FEV; FLI1)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

Constitutively activating Ets1/2 driven in B7.5 cell pair by the mesp enhancer. The Ets1/2 DNA-binding domain was amplified from an Ets1/2 EST clone (R1CiGC01h16) obtained from the Ciona intestinalis Gene Collection Release 1 (Satou et al. 2005) using the follow-ing primers (lower case indicates restriction site and flanking base pairs):EtsDBDf, aaagctagcGCCTATACTGACACCTTTGAAGCC ; EtsDBDr, aaaactagtATCATCACTTAGTCGATCTTCAGGC. It was then fused in-frame into modified pCES vectors containing the full-length Mesp enhancer and 3'activator (Vp16) domain.

Regulation

Gain of function

Publication

Beh et al. (2007). FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. Development 134(18):3297-305

Davidson et al. (2006). FGF signaling delineates the cardiac progenitor field in the simple chordate, Ciona intestinalis. Genes Dev. 20(19):2728-38

Woznica et al. (2012). Initial deployment of the cardiogenic gene regulatory network in the basal chordate, Ciona intestinalis. Dev Biol 368(1):127-39