Name

ETR>Ngn-VP16

Targeted Gene

(NEUROG1; NEUROG2; NEUROG3)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

This construct over-expresses a constitutively activator (Ngn–VP16) form of Neurogenin in the whole nervous system.
The Etr–Ngn–VP16 construct was prepared by excising the Neurogenin CDS contained in the pCRsII vector through digestion with NheI and SpeI restriction enzymes and replacing the Onecut coding sequence in the Etr–OC– VP16 as previously described (D’Aniello et al., 2011).

Regulation

Gain of function

Publication

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87