- Molecular Tool 'Dmrt>BMP2/4'
Molecular tool card for 'Dmrt>BMP2/4'
Name
Dmrt>BMP2/4
Targeted Gene
Family
Sequence-based
Type
overexpression_construct
Supplier
n/a
Comment
Dmrt enhancer (sequence below) drives BMP2/4 expression throughout the anterior neural plate and adjacent anterior ectoderm. This misexpression vectors was built using NotI/EcoRI sites for BMP2/4 (KH.C4.125; 59-ATGGTGGCGCTTACGGATTGGAC-39 and 59-CTATCTACACCCACAAGCTTGC-39).
Regulation
Gain of function
>KhS544:4240-5239_Dmrttagtagggtggaggaagatgggacacctttggcacata
atatccaaatatccttaccccgttttacacaattaacagcggtatatgggagtcgtaaga
attccgttttataattatttgcatgttctttgtttgctactaaattggacgacaaaatgt
agtcaaaaggtgtctaatcttcccctaccctactatacgacttggggcatagttataaag
ttattccgtttcatttcttggcgttaacccgacgatcagtaattaacgatattcttcata
aataaataatgcgagaactgcaacaggactagggaagttacttaagcgagcatgtgatta
gaaattgccactgaagcactttaaaatgccgccaaaacacatacaccttcatgacacatt
gttttacagaatctcaatttaaaacccgtcgacaattatcggatttccgccgcagagtca
caggaagttgttttgcttaataatacttgttttagataagctgttatgttgctcaatgaa
taacaataatgtttacgttggttgttgtggcaatgtacattgtaacttccacaaacctct
cagagggcaaacacattaagcaaactgaatttgccaagtacttcgggaagtcactgataa
gcggacaatacgaagcgatcacagggaattgtcgcacgccagaatgtaaacaaaaacatc
gcctgtgcaccgcgcgatcgaagacacacagcgctgactaattgaccaacacaaaagcga
ggaaagaaaaaaaagcgaaaacgagaacgggatattaattgtacacgattgtgacgtaaa
ttcgaggcattttttcttccgaaatcgagcggtttaaatacctcgcgtcttt
