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  1. Transcript 'Phfumi.CG.MTP2014.S5...' > 
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  4. Molecular Tool 'bFGF' > 
  5. Molecular Tool 'DMRT1>ZicL'

Molecular tool card for 'DMRT1>ZicL'

Name

DMRT1>ZicL

Targeted Gene

(ZIC2; ZIC3; ZIC4)

Family

Sequence-based

Type

overexpression_construct

Supplier

n/a

Comment

This constuct is used to express ZicL under the DMRT1 upstream sequence, leading to overexpression in the a neural plate. The cis-regulatory sequence of DMRT1 used in this construct is shown below.

Regulation

Gain of function

Publication

Ikeda et al. (2013). A time delay gene circuit is required for palp formation in the ascidian embryo. Development 140(23):4703-8

Sequence

>Ciinte.REG.KH2012.S544:4212-5239|DMRT1cttcgccggagagttaaaatc
atcattatagtagggtggaggaagatgggacacctttggcacataatatccaaatatcct
taccccgttttacacaattaacagcggtatatgggagtcgtaagaattccgttttataat
tatttgcatgttctttgtttgctactaaattggacgacaaaatgtagtcaaaaggtgtct
aatcttcccctaccctactatacgacttggggcatagttataaagttattccgtttcatt
tcttggcgttaacccgacgatcagtaattaacgatattcttcataaataaataatgcgag
aactgcaacaggactagggaagttacttaagcgagcatgtgattagaaattgccactgaa
gcactttaaaatgccgccaaaacacatacaccttcatgacacattgttttacagaatctc
aatttaaaacccgtcgacaattatcggatttccgccgcagagtcacaggaagttgttttg
cttaataatacttgttttagataagctgttatgttgctcaatgaataacaataatgttta
cgttggttgttgtggcaatgtacattgtaacttccacaaacctctcagagggcaaacaca
ttaagcaaactgaatttgccaagtacttcgggaagtcactgataagcggacaatacgaag
cgatcacagggaattgtcgcacgccagaatgtaaacaaaaacatcgcctgtgcaccgcgc
gatcgaagacacacagcgctgactaattgaccaacacaaaagcgaggaaagaaaaaaaag
cgaaaacgagaacgggatattaattgtacacgattgtgacgtaaattcgaggcatttttt
cttccgaaatcgagcggtttaaatacctcgcgtctttattaggtacaatcatattgcctt
gataccttgcttagaacagtgaatacatataaatcgtattaaaagctttagagtgttaaa
ccaaagagttacagaaattatagcaaacagttcgtttaactggcatg

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