1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Phallusia mammillata (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
8 results
Vegetal view of a WT Phallusia mammillata 16-cell stage embryo probed for Nodal expression.
Nodal is not yet expressed at this stage.
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | No expression - |
Vegetal (left) and animal (right) views of a WT Phallusia mammillata 32-cell stage embryo probed for Nodal expression.
Nodal is expressed in A6.1, A6.3, A6.4 and b6.5 blastomer pairs.
Original comment: Nodal is transiently expressed throughout the vegetal hemisphere at the 32-cell stage, and its expression pattern is perfectly conserved between Ciona and Phallusia.
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | A6.1 cell pair - A6.3 cell pair - A6.4 cell pair - b6.5 cell pair - |
Vegetal view of a WT Phallusia mammillata 64-cell stage embryo probed for Nodal expression.
Nodal is expressed in b7.9 and b7.10 blastomer pairs.
Original comment: The expression pattern of Nodal is perfectly conserved between Ciona and Phallusia.
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | b7.10 cell pair - b7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 76-cell stage embryo probed for Nodal expression.
Nodal is expressed in b7.9 and b7.10 blastomer pairs.
Original comment: The expression pattern of Nodal is perfectly conserved between Ciona and Phallusia.
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | b7.10 cell pair - b7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 110-cell stage embryo probed for Nodal expression.
Nodal is expressed in b8.17, b8.18, b8.19 and b8.20 blastomer pairs.
Original comment: The expression pattern of Nodal is perfectly conserved between Ciona and Phallusia.
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - |
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | anterior sensory vesicle - posterior sensory vesicle - |
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | endoderm - notochord - tail muscles - |
Primary expression : mesenchyme
Secondary expression : muscle and nerve cord
| Stained molecule | Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL) |
| Stained region(s) | tail muscles - tail nerve cord - |
