1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Phallusia mammillata (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
6 results
Lateral view of a WT Phallusia mammillata mid tailbud stage embryo probed for Mycn expression.
Mycn (a late endoderm marker) is expressed in the head endoderm.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a Phallusia mammillata mid tailbud stage embryo probed for Mycn expression and treated with 6 μM NVPBHG712 from the 8-cell stage to inhibit Eph-mediated signalling.
Mycn is expressed in the head endoderm.
Original comment: This treatment blocked endoderm invagination without preventing the expression of the late vegetal endoderm marker Mycn.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a WT Phallusia mammillata mid tailbud stage embryo probed for Mycn expression.
Mycn (a late endoderm marker) is expressed in the head endoderm.
Scale bar: 50 μm.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a WT Phallusia mammillata mid tailbud stage embryo probed for Mycn expression.
Mycn (a late endoderm marker) is expressed in the head endoderm.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a WT Phallusia mammillata mid tailbud stage embryo probed for Mycn expression.
Mycn (a late endoderm marker) is expressed in the head endoderm.
Scale bar: 50 μm.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a WT Phallusia mammillata mid tailbud stage embryo probed for Mycn expression.
Mycn (a late endoderm marker) is expressed in the head endoderm.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a Phallusia mammillata mid tailbud stage embryo probed for Mycn expression and treated from the 64-cell stage with the ROCK inhibitor Y27632 (blockage of step 1 of endoderm invagination).
Mycn (a late endoderm marker) is expressed in the head endoderm.
Original comment: Inhibition of Rock, the main driver of the first step of invagination, did not affect endoderm specification.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
Lateral view of a Phallusia mammillata mid-tailbud stage embryo probed for Mycn expression and treated from the 16-cell stage with SB431542, a selective pharmacological inhibitor of the Nodal receptor ALK4/5/7.
Mycn is expressed in the head endoderm.
Original comment: Expression level of the late vegetal
endodermal marker Mycn was not reduced by Nodal inhibition.
| Stained molecule | Phmamm.CG.MTP2014.S309.g07048 (MAX; MYC; MYCN) |
| Stained region(s) | head endoderm - |
