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RNA-Seq transcriptome profiles (by Experiment)

1 result

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34136

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Oocytes of a single individual of Phallusia mammillata (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) P.mammillata, Stage 0 (Unfertilized egg)
P.mammillata, Stage 8 (64-cell)
P.mammillata, Stage 11 (early gastrula)
P.mammillata, Stage 12 (mid gastrula)
P.mammillata, Stage 15 (mid neurula)
P.mammillata, Stage 21 (mid tailbud I)
P.mammillata, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) P.mammillata, Stage 0 (Unfertilized egg)
P.mammillata, Stage 8 (64-cell)
P.mammillata, Stage 11 (early gastrula)
P.mammillata, Stage 12 (mid gastrula)
P.mammillata, Stage 15 (mid neurula)
P.mammillata, Stage 21 (mid tailbud I)
P.mammillata, Stage 26 (hatching larva)

In Situ Experiment Data

21 results

Experiment data ( results)

P.mammillata, Stage 0 (Unfertilized egg)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) No expression -

P.mammillata, Stage 4 (8-cell)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) No expression -

P.mammillata, Stage 5a (early 16-cell)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) A5.1 cell pair - A5.2 cell pair - B5.1 cell pair -

P.mammillata, Stage 10 (112-cell)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) No expression -

P.mammillata, Stage 11 (early gastrula)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D
Expression in B-line endoderm.

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) B7.2 cell pair -

P.mammillata, Stage 12 (mid gastrula)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) endodermal strand -

P.mammillata, Stage 13 (late gastrula)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) endodermal strand -

P.mammillata, Stage 14 (early neurula)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) endodermal strand -

P.mammillata, Stage 15 (mid neurula)

Read more…

Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) endodermal strand -

P.mammillata, Stage 16 (late neurula)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s)

P.mammillata, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) No expression -

P.mammillata, Stage 21 (mid tailbud I)

Read more…

Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) posterior sensory vesicle - visceral ganglion -

P.mammillata, Stage 22 (mid tailbud II)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) posterior sensory vesicle - visceral ganglion -

P.mammillata, Stage 23 (late tailbud I)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) posterior sensory vesicle - tail epidermis - tail nerve cord -

P.mammillata, Stage 24 (late tailbud II)

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Conversion to Aniseed format of data from Remi Dumollard et al. (Ascidian BioCell Team, LBDV, Villefranche-sur-mer, France).
Gene name : fox D

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) posterior sensory vesicle - tail nerve cord -

P.mammillata, Stage 26 (hatching larva)

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Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) anterior sensory vesicle - endoderm - tail muscles -

P.mammillata, Stage 26 (hatching larva)

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Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) tail muscles -

P.mammillata, Stage 26 (hatching larva)

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Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) trunk lateral cells (A7.6 line) -

P.mammillata, Stage 26 (hatching larva)

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Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) posterior sensory vesicle - tail muscles -

P.mammillata, Stage 26 (hatching larva)

Read more…

Stained molecule Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)
Stained region(s) anterior sensory vesicle - posterior sensory vesicle - tail muscles -