1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Phallusia mammillata (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | P.mammillata, Stage 0 (Unfertilized egg) | |
| P.mammillata, Stage 8 (64-cell) | ||
| P.mammillata, Stage 11 (early gastrula) | ||
| P.mammillata, Stage 12 (mid gastrula) | ||
| P.mammillata, Stage 15 (mid neurula) | ||
| P.mammillata, Stage 21 (mid tailbud I) | ||
| P.mammillata, Stage 26 (hatching larva) | ||
9 results
Vegetal view of a WT Phallusia mammillata 16-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the vegetal hemisphere.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A line - B line - |
Vegetal view of a WT Phallusia mammillata 32-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in A6.2 and A6.4 and very weakly in A6.1, A6.3 and B6.2.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - |
Vegetal view of a WT Phallusia mammillata 64-cell stage embryo probed for Efna.c expression. Embryo scheme shows Efna.c expression (red) in vegetal (right) and animal (left) hemispheres.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in all animal hemisphere except a7.15, b7.15 and b7.16..
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | a7.10 cell pair - a7.11 cell pair - a7.12 cell pair - a7.13 cell pair - a7.14 cell pair - a7.16 cell pair - A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a7.9 cell pair - b7.10 cell pair - b7.11 cell pair - b7.12 cell pair - b7.13 cell pair - b7.14 cell pair - b7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 76-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a7.9, a7.10 and a7.13 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | a7.10 cell pair - a7.13 cell pair - A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 76-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a7.9, a7.10 and a7.13 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | a7.10 cell pair - a7.13 cell pair - A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 76-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a7.9, a7.10 and a7.13 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | a7.10 cell pair - a7.13 cell pair - A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a7.9 cell pair - |
Vegetal view of a WT Phallusia mammillata 76-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a7.9, a7.10 and a7.13 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | a7.10 cell pair - a7.13 cell pair - A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a7.9 cell pair - |
Vegetal view of a Phallusia mammillata 76-cell stage embryo probed for Efna.c expression and treated from the 16-cell stage with SB431542, a selective pharmacological inhibitor of the Nodal receptor ALK4/5/7.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs.
Original comment: Efna.c is expressed in A-line endodermal cells from the 76-cell stage. This expression is not affected by Nodal signalling inhibition.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - |
Vegetal view of a WT Phallusia mammillata 112-cell stage embryo probed for Efna.c expression. Embryo scheme shows Efna.c expression (red) in vegetal (right) and animal (left) hemispheres.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a8.17, a8.19, a8.18, a8.20, a8.26 and a8.25 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.25 cell pair - a8.26 cell pair - |
Vegetal view of a WT Phallusia mammillata 112-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs, staining in the animal hemisphere cannot be observed.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - |
Vegetal view of a WT Phallusia mammillata 112-cell stage embryo probed for Efna.c expression. Embryo scheme shows Efna.c expression (red) in vegetal (right) and animal (left) hemispheres.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs and in a8.17, a8.19, a8.18, a8.20, a8.26 and a8.25 animal blastomeres.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.25 cell pair - a8.26 cell pair - |
Vegetal view of a WT Phallusia mammillata 112-cell stage embryo probed for Efna.c expression.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs, staining in the animal hemisphere cannot be observed.
Original comment: The gene encoding the ephrinA ligand Efna.c is expressed in the A-line endoderm from the 64-cell stage.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - |
Vegetal view of a Phallusia mammillata 112-cell stage embryo probed for Efna.c expression and treated from the 16-cell stage with SB431542, a selective pharmacological inhibitor of the Nodal receptor ALK4/5/7.
Efna.c is expressed in the A7.1, A7.2 and A7.5 blastomere pairs, no image of the animal hemisphere is provided.
Original comment: Efna.c is expressed in A-line endodermal cells from the 76-cell stage. This expression is not affected by Nodal signalling inhibition.
| Stained molecule | Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - |
