Dorso-lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Mnx (green) and Islet (pink) expressions. Mnx is expressed in A11.118 and A10.64 cell pairs.
Orignal annotation : Two-color fluorescent in situ hybridization for Mnx (green) and MN2 marker Islet (magenta), revealing identities of MN1 (Mnx+/Islet-) and MN2 (Mnx+/Islet+) cells. Boxed area magnified in insets.
Anterior is to the left. Scale bar = 25 µm

Dorso-lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Islet (pink) and Mnx (green) expressions. Islet is expressed in A10.64 cell pair and in the notochord.

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Vsx (green) and Islet (pink) expressions. Vsx is expressed in A11.119 and A11.117 cell pairs.
Orignal annotation : Interneuron marker Vsx (green) is expressed in undifferentiated A11.119 interneuron progenitor cell and interneuron 2 (IN2).
Boxed area magnified in insert. Anterior is to the left. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Islet (pink) and Vsx (green) expressions. Islet is expressed in A10.64 cell pair (or motor neuron 2 MN2) and in the notochord.
Boxed area magnified in insert. Anterior is to the left. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Islet (magenta) expressions. Lhx3/4 is expressed in the visceral ganglion, in the motor neuron 2 (MN2) and in the anterior sensory vesicle.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Islet (magenta) showing co-expression in MN2.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm

Lateral view of a WT Molgula occidentalis mid tailbud embryo probed for Lhx3/4 (green) and Islet (magenta) expressions. Islet is expressed in the motor neuron 2 (MN2) and in the notochord.
Orignal annotation : Two-color fluorescent in situ hybridization for Lhx3/4.a (green) and Islet (magenta) showing co-expression in MN2.
Anterior is to the left. Boxed area is enlarged in inserts. Scale bar = 25 µm