1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
8 results
WT Halocynthia roretzi fertilized egg probed for FoxDb expression.
FoxDb is not yet expressed.
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
WT Halocynthia roretzi 4-cell stage embryo probed for FoxDb expression.
FoxDb is not yet expressed.
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 16-cell stage embryo probed for FoxDb expression.
FoxDb is not yet expressed.
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 32-cell stage embryo probed for FoxDb expression.
FoxDb is not yet expressed.
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 64-cell stage embryo probed for FoxDb expression.
FoxDb is not yet expressed.
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 110-cell stage embryo probed for FoxDb expression.
FoxDb is not yet expressed
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view with anterior up of a WT Halocynthia roretzi neurula stage embryo probed for FoxDb expression.
Original comment: Hr-FoxDb is first detected at the neurula stage, the expression being strong in the dorsal region around the closing neuropore (top center arrowhead) and weak in four separate regions of the dorsolateral part of the embryo (4 other arrowheads).
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | dorsal midline anterior tail epidermis - dorso lateral anterior tail epidermis - neural plate - |
Lateral (U) and dorsal (V) views of a WT Halocynthia roretzi 64-cell stage embryo probed for FoxDb expression.
FoxDb strong staining can be seen in four spots around the neck region (arrowheads).
| Stained molecule | Harore.CG.MTP2014.S128.g13119 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | dorsal midline anterior tail epidermis - dorso lateral anterior tail epidermis - |
