1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
18 results
Vegetal view of a WT Halocynthia roretzi 16-cell stage embryo probed for Tbx6 expression.
Tbx6 is not yet expressed at this stage.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal (A) and posterior (B) views of a Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression, injected with a control MO and treated with DMSO.
Tbx6 is expressed in B6.1, B6.2 and B6.4.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal (F) and posterior (G) views of a WT Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B6.1, B6.2 and B6.4.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal (A) and posterior (B) views of a Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression, injected with a control MO and treated with DMSO.
Tbx6 is expressed in B6.1, B6.2 and B6.4.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal (F) and posterior (G) views of a WT Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B6.1, B6.2 and B6.4.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression, injected with Macho-1 MO.
Tbx6 expression is lost.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal (M) and posterior (N) views of a Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression and injected with Tbx6 MO.
Tbx6 is expressed in B6.1, B6.2 and B6.4, as in WT conditions.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal (M) and posterior (N) views of a Halocynthia roretzi 32-cell stage embryo probed for Tbx6 expression and treated with the MEK inhibitor U0126.
Tbx6 is expressed in B6.1, B6.2 and B6.4, as in WT conditions.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.4 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression and injected with control MO and treated with DMSO.
Tbx6 is expressed in B7.2, B7.4, B7.5, B7.7 and B7.8.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.2 cell pair - B7.4 cell pair - B7.5 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a WT Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B7.2, B7.4, B7.1, B7.7 and B7.8.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.1 cell pair - B7.2 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression and injected with control MO and treated with DMSO.
Tbx6 is expressed in B7.2, B7.4, B7.5, B7.7 and B7.8.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.2 cell pair - B7.4 cell pair - B7.5 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a WT Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B7.2, B7.4, B7.1, B7.7 and B7.8.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.1 cell pair - B7.2 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression and injected with Macho-1 MO.
Tbx6 expression is lost.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression and injected with Tbx6 MO.
Tbx6 expression is lost.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for Tbx6 expression and treated with the MEK inhibitor U0126.
Tbx6 is expressed in B7.1, B7.2, B7.4, B7.7 and B7.8, and ectopically in B7.3 mesenchyme precursor cell.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.1 cell pair - B7.2 cell pair - B7.3 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression, injected with a control MO and treated with DMSO.
Tbx6 is expressed in B7.5, B8.8, B8.7, B8.15 and B8.16.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B7.5, B8.8, B8.7, B8.15 and B8.16.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression, injected with a control MO and treated with DMSO.
Tbx6 is expressed in B7.5, B8.8, B8.7, B8.15 and B8.16.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression.
Tbx6 is expressed in B7.5, B8.8, B8.7, B8.15 and B8.16.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression and treated with the MEK inhibitor U0126.
Tbx6 is expressed in B7.5, B8.6, B8.8, B8.7, B8.15 and B8.16, and ectopically in B8.5 and B7.7 blastomeres.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression and injected with Macho1 MO.
Tbx6 is no longer expressed.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression and injected with Tbx6 MO.
Tbx6 is no longer expressed.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | No expression - |
Vegetal view of a control Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression and treated with DMSO
Tbx6 is expressed in B7.5, B8.15, B8.16, B8.7 and B8.8 blastomer pairs.
White arrows indicate the absence of ectopic gene expression in germline cells.
The numbers represent the proportion of embryos showing ectopic gene expression in the germline (B7.6).
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Tbx6 expression, injected with morpholinos against Pem and treated with GSK126, a selective inhibitor of EZH2 used to inhibit H3K27me3.
Tbx6 is expressed in B7.5, B8.15, B8.16, B8.7 and B8.8 blastomer pairs, as in WT conditions.
Original comment: Ectopic Tbx6 expression was not observed in germlines deficient in Pem- and H3K27me3 ( 1.96%, n = 51).
White arrows indicate the absence of ectopic gene expression in germline cells.
| Stained molecule | Harore.CG.MTP2014.S461.g12970 (TBX2; TBX4; TBX6) |
| Stained region(s) | B7.5 cell pair - B8.15 cell pair - B8.16 cell pair - B8.7 cell pair - B8.8 cell pair - |
