Aniseed - Gene Card

EST count

No result

RNA-Seq transcriptome profiles (by Experiment)

1 result

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

Relative Log Expression RLE

Be careful, RLE and 2^RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34137

Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).

Assay platform	Illumina HiSeq 2000
Layout	Paired-end
Stranded	Yes
Read length	49
Normalized data types	FPKM RLE
Wild type - Replica 1
	*Territories*	whole embryo -

	*Biomaterial(s)*	H.roretzi, Stage 0 (Unfertilized egg)
		H.roretzi, Stage 8 (64-cell)
		H.roretzi, Stage 12 (mid gastrula)
		H.roretzi, Stage 21 (mid tailbud I)
		H.roretzi, Stage 26 (hatching larva)

Wild type - Replica 2
	*Territories*	whole embryo -

	*Biomaterial(s)*	H.roretzi, Stage 0 (Unfertilized egg)
		H.roretzi, Stage 8 (64-cell)
		H.roretzi, Stage 12 (mid gastrula)
		H.roretzi, Stage 15 (mid neurula)
		H.roretzi, Stage 21 (mid tailbud I)
		H.roretzi, Stage 26 (hatching larva)

In Situ Experiment Data

33 results

Experiment data ( results)

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 5a (early 16-cell)

An image of a 16-cell stage Halocynthia roretzi embryo stained with anti-beta-catenin antibody. A weak signal is observed in the nucleus of B5.2 blastomeres (white arrow heads 1st image) as well as at the CAB. All the other vegetal cells are stained much stronger, as observed in the second picture from a different focus plan. Vegetal view with anterior up.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair -

H.roretzi, Stage 5a (early 16-cell)

Staining in B5.2 increases and becomes much stronger upon PEM MO injection. Vegetal view with anterior up of a 16-cell stage Halocynthia roretzi embryo immunostained for beta-catenin localization .

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair -

H.roretzi, Stage 5a (early 16-cell)

Vegetal view with anterior up of a Halocynthia roretzi 16-cell stage embryo immunostained for β-catenin localization and injected with control YFP mRNA.
β-catenin is localized in the vegetal hemisphere. White arrowheads indicate the position of nuclei.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - A line - B5.1 cell pair - B5.2 cell pair - B line -

H.roretzi, Stage 5a (early 16-cell)

Vegetal view with anterior up of a Halocynthia roretzi 16-cell stage embryo immunostained for β-catenin localization and injected with PEM MO.
β-catenin is localized in vegetal blastomers.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	A5.1 cell pair - A5.2 cell pair - A line - B5.1 cell pair - B5.2 cell pair - B line -

H.roretzi, Stage 5a (early 16-cell)

Vegetal view with anterior up of a Halocynthia roretzi 16-cell stage embryo immunostained for beta-catenin localization and injected with PEM and β-catenin morpholinos.
The authors observed the disappearance of β-catenin staining from the nucleus. White arrowheads indicate the position of nuclei.
The numbers represent the number of embryos with little or no signals.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	No expression -

H.roretzi, Stage 5a (early 16-cell)

Anterior-vegetal views with anterior up of a Halocynthia roretzi 16-cell stage embryo immunostained for beta-catenin localization and injected with PEM mRNA.
Original annotation: Forced expression of PEM mRNA in cells that normally express Hr-foxDa decreased the level of nuclear β-catenin (87% (n = 23), yellow arrowheads (affected side) vs. white arrowheads (unaffected side).
The numbers represent the number of embryos with little or no signals.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	No expression -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : epidermis, brain, nerve cord, A-line notochord, B-line notochord, muscle, mesenchyme, trunk lateral cell, trunk ventral cell, endoderm.

Stained molecule

Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)

Stained region(s)

A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : epidermis, brain, mesenchyme.

Stained molecule

Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)

Stained region(s)

a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.7 cell pair - A8.8 cell pair - B7.7 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : epidermis, brain, nerve cord, muscle, mesenchyme.

Stained molecule

Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)

Stained region(s)

A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.7 cell pair - A8.8 cell pair - B7.5 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.7 cell pair - B8.8 cell pair -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : epidermis, brain, nerve cord, A-line notochord, B-line notochord, muscle, mesenchyme, trunk ventral cell, endoderm.

Stained molecule

Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)

Stained region(s)

A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : no expression.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	No expression -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : nerve cord.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	tail nerve cord -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : adhesive organ, brain, nerve cord, mesenchyme.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	anterior sensory vesicle - Palps - posterior sensory vesicle - tail nerve cord -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : epidermis, adhesive organ.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	epidermis - Palps -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : brain, nerve cord, muscle.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	anterior sensory vesicle - posterior sensory vesicle - tail muscles - tail nerve cord -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : adhesive organ, brain, nerve cord, mesenchyme.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	anterior sensory vesicle - Palps - posterior sensory vesicle - tail nerve cord -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : overall.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	whole embryo -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : epidermis, adhesive organ, brain, nerve cord, mesenchyme.

Stained molecule	Harore.CG.MTP2014.S183.g11196 (ARMC4; CTNNB1; JUP)
Stained region(s)	anterior sensory vesicle - epidermis - Palps - posterior sensory vesicle - tail nerve cord -