1 result
OVERVIEW
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OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
32 results
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for brachyury expression and injected with a control MO.
Brachyury is expressed in A7.3 and A7.7 blastomer pairs.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A7.3 cell pair - A7.7 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for brachyury expression and injected with Fgf9/16/20 MO.
Brachyury expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for brachyury expression and injected with FoxA MO.
Brachyury expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for brachyury expression and injected with ZicN MO.
Brachyury expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal views of WT Halocynthia roretzi 110-cell stage embryos with brachyury expression visualized by in situ hybridization.
Brachyury is expressed in A8.13, A8.14, A8.5, A8.6 and B8.6 blastomere pairs.
The expression is absent in B8.5 (black or red arrow heads) and B7.7 (white arrow heads) mesenchyme-lineage cells.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B8.6 cell pair - |
Vegetal view of Halocynthia roretzi 110-cell stage embryos probed for brachyury expression and injected with PEM MO.
Ectopic expression is observed in B8.5 (black or red arrowheads) and B7.7 (whitearrow heads) blastomeres and other cells in the posterior half of the vegetal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.7 cell pair - B8.5 cell pair - B8.6 cell pair - B line - |
Vegetal view with anterior up of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with PEM and ZicN MOs.
Expression is all gone.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view with anterior up of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with PEM and FoxA MOs.
Expression is all gone.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view with anterior up of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with PEM mRNA.
Expression is eliminated.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view with anterior up of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with Macho-1 mRNA.
Expression is absent on the injected side.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression.
The embryo showed Harore-Bra expression in A8.5, A8.6, A8.13, A8.14 and B8.6 blastomers.
Percentage of embryos showing ectopic expression is indicated with the total number of specimens in the bottom right-hand corner.
Scale bar: 100 µm
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B8.6 cell pair - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression and injected with FoxB morpholino.
The embryo showed Harore-Bra expression in A8.5, A8.6, A8.13, A8.14, A8.16, B8.5 and B8.6 blastomers.
Original annotation: Ectopic expression of Hr-Bra in likely descendent cells of A7.8 was evident in only three out of 26 cases of 110-cell embryos injected with FoxB MO (arrowhead).
Percentage of embryos showing ectopic expression is indicated with the total number of specimens in the bottom right-hand corner.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.16 cell pair - A8.5 cell pair - A8.6 cell pair - B8.5 cell pair - B8.6 cell pair - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression and injected with 50 pg of control YFP mRNA.
The embryo showed Harore-Bra expression in A8.13, A8.14, A8.5, A8.6 and B8.6 blastomers.
Scale bar: 100 µm
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B8.6 cell pair - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression and injected with FoxB mRNA.
Brachyury expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression and injected with FoxB:EnR mRNA (mRNA encoding Hr-FoxB conjugated with the EnR transcription repression domain).
Brachyuru expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a 110-cell stage Halocynthia roretzi embryo probed for Brachyury expression and injected with FoxB:VP16 mRNA (mRNA encoding Hr-FoxB conjugated with the VP16 transcription activation domain).
Brachyury expression is observed in WT territories (A8.5, A8.6, A8.13, A8.14 and B8.6), and ectopically in B8.5, B7.5 and B7.7.
Original annotation: VP16-conjugated FoxB activated the ectopic expression of Hr-Bra in many cells in the vegetal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.5 cell pair - B7.7 cell pair - B8.5 cell pair - B8.6 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with a control MO.
Brachyury is expressed in A8.5, A8.6, A8.13, A8.14 and B8.6 blastomer pairs.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A7.5 cell pair - A7.6 cell pair - A8.13 cell pair - A8.14 cell pair - B8.6 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with Fgf9/16/20 MO.
Brachyury’s expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with FoxDa MO.
Brachyury’s expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with FoxA MO.
Brachyury’s expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with ZicN MO.
Brachyury’s expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for brachyury expression and injected with a control MO.
Brachyury is expressed in A8.5, A8.6, A8.13, A8.14 and B8.6 blastomer pairs.
Scale bar: 100 μm.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B8.6 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Fgf9/16/20 MO.
Brachyury's expression is lost.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA.
Brachyury is expressed in A8.5, A8.6, A8.13, A8.14 and B8.6, and ectopically in B7.1, B7.2 and B7.7 blastomeres.
Original comment: Hr-Bra was ectopically expressed in the posterior mesenchyme cells as well as the posterior endoderm cells upon Hr-FoxA over-expression (75%, n = 16).
Diagram: 110-cell embryo illustrating in purple the expression of Hr-Bra.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.1 cell pair - B7.2 cell pair - B7.7 cell pair - B8.6 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA and a control MO.
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with DMSO after fertilization (control).
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA and Hr-ZicN MO.
Brachyury's expression is lost.
Original comment: The ectopic Hr-Bra expression driven by Hr-FoxA over-expression is dependent on Hr-ZicN.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA and Hr-ZicN MO.
Brachyury's expression is lost.
Original comment: Co-injection of Hr-ZicN MO with Hr-FoxA RNA resulted in reduction of the ectopic Hr-Bra expression in the animal region of the whole embryo (22% positive, n = 9) .
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with the MEK inhibitor U0126 from the 1-cell stage.
Brachyury's expression is lost.
Original comment: The ectopic Hr-Bra expression driven by Hr-FoxA over-expression is dependent on FGF signalling.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with the MEK inhibitor U0126 from the 1-cell stage.
Brachyury's expression is lost.
Original comment: Hr-Bra induction by Hr-FoxA is dependent on FGF signaling in the whole embryo (0% positive, n = 9) with UO126 treatment.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA.
Brachyury is expressed in A8.5, A8.6, A8.13, A8.14 and B8.6, and ectopically in B7.1, B7.2 and B7.7 blastomeres.
Original comment: Hr-Bra was ectopically expressed in the posterior mesenchyme cells as well as the posterior endoderm cells upon Hr-FoxA over-expression (75%, n = 16).
Diagram: 110-cell embryo illustrating in purple the expression of Hr-Bra.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.1 cell pair - B7.2 cell pair - B7.7 cell pair - B8.6 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA and a control MO.
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with DMSO after fertilization (control).
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA.
Brachyury is expressed in A8.5, A8.6, A8.13, A8.14 and B8.6, and ectopically in B7.1, B7.2 and B7.7 blastomeres.
Original comment: Hr-Bra was ectopically expressed in the posterior mesenchyme cells as well as the posterior endoderm cells upon Hr-FoxA over-expression (75%, n = 16).
Diagram: 110-cell embryo illustrating in purple the expression of Hr-Bra.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.1 cell pair - B7.2 cell pair - B7.7 cell pair - B8.6 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and injected with Hr-FoxA mRNA and a control MO.
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with DMSO after fertilization (control).
Brachyury is ectopically expressed in the animal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | a line - b line - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with the MEK inhibitor U0126 from the 1-cell stage.
Brachyury's expression is lost.
Original comment: The ectopic Hr-Bra expression driven by Hr-FoxA over-expression is dependent on FGF signalling.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with Hr-FoxA mRNA and treated with the MEK inhibitor U0126 from the 1-cell stage.
Brachyury's expression is lost.
Original comment: Hr-Bra induction by Hr-FoxA is dependent on FGF signaling in the whole embryo (0% positive, n = 9) with UO126 treatment.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a control Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression and treated with DMSO.
Brachyury is expressed in B8.6, A8.5, A8.6, A8.13 and A8.14 blastomer pairs (notochord precursors) and sometimes in B8.5 (grey arrow, mesenchyme precursors).
Yellow arrows indicate the absence of ectopic gene expression in germline cells.
The numbers represent the proportion of embryos showing ectopic gene expression in the germline.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | A7.5 cell pair - A8.13 cell pair - A8.14 cell pair - A8.6 cell pair - B8.5 cell pair - B8.6 cell pair - |
Lateral view of a Halocynthia roretzi mid tailbud stage embryo probed for brachyury expression and injected with a control MO.
Brachyury is expressed in the notochord and weak background staining is observed in muscle cells.
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | notochord - |
Halocynthia roretzi mid tailbud stage embryos probed for brachyury expression and injected with Hr-Fgf9/16/20 MO.
Brachyury's expression is lost.
Original comment: Knockdown of Hr-Fgf9/16/20 by MO injection resulted in almost complete loss of notochord-specific Hr-Bra expression at the mid-tailbud stage (91%, n= 11).
| Stained molecule | Harore.CG.MTP2014.S42.g07916 (TBR1; TBX19; TBXT) |
| Stained region(s) | No expression - |
