Login
Help

GENE CARD

Submit your Data

EST count

No result

RNA-Seq transcriptome profiles (by Experiment)

1 result

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34137

Read more…

Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) H.roretzi, Stage 0 (Unfertilized egg)
H.roretzi, Stage 8 (64-cell)
H.roretzi, Stage 12 (mid gastrula)
H.roretzi, Stage 21 (mid tailbud I)
H.roretzi, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) H.roretzi, Stage 0 (Unfertilized egg)
H.roretzi, Stage 8 (64-cell)
H.roretzi, Stage 12 (mid gastrula)
H.roretzi, Stage 15 (mid neurula)
H.roretzi, Stage 21 (mid tailbud I)
H.roretzi, Stage 26 (hatching larva)

In Situ Experiment Data

34 results

Experiment data ( results)

H.roretzi, Stage 4 (8-cell)

Read more…

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

Read more…

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

Read more…

MAGEST annotation : a4.2, A4.1.

Stained molecule Harore.CG.MTP2014.S50.g14944 (Harore.CG.MTP2014.S50.g14944)
Stained region(s) A4.1 cell pair - a4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

Read more…

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 4 (8-cell)

Read more…

MAGEST annotation : a4.2, b4.2, A4.1, B4.1.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A4.1 cell pair - a4.2 cell pair - B4.1 cell pair - b4.2 cell pair -

H.roretzi, Stage 10 (110-cell)

Read more…

MAGEST annotation : epidermis, brain, nerve cord, A-line notochord, B-line notochord, muscle, mesenchyme, trunk lateral cell.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A7.6 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B7.5 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair -

H.roretzi, Stage 10 (110-cell)

Read more…

MAGEST annotation : epidermis, brain, nerve cord, A-line notochord, B-line notochord, muscle, mesenchyme, trunk lateral cell, trunk ventral cell, endoderm.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.25 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair -

H.roretzi, Stage 10 (110-cell)

Read more…

MAGEST annotation : epidermis.

Stained molecule Harore.CG.MTP2014.S50.g14944 (Harore.CG.MTP2014.S50.g14944)
Stained region(s) a8.18 cell pair - a8.20 cell pair - a8.21 cell pair - a8.22 cell pair - a8.23 cell pair - a8.24 cell pair - a8.26 cell pair - a8.27 cell pair - a8.28 cell pair - a8.29 cell pair - a8.30 cell pair - a8.31 cell pair - a8.32 cell pair - b8.18 cell pair - b8.20 cell pair - b8.21 cell pair - b8.22 cell pair - b8.23 cell pair - b8.24 cell pair - b8.25 cell pair - b8.26 cell pair - b8.27 cell pair - b8.28 cell pair - b8.29 cell pair - b8.30 cell pair - b8.31 cell pair - b8.32 cell pair -

H.roretzi, Stage 10 (110-cell)

Read more…

MAGEST annotation : no expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) No expression -

H.roretzi, Stage 10 (110-cell)

Read more…

MAGEST annotation : no expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) No expression -

H.roretzi, Stage 12 (mid gastrula)

Read more…

Vegetal view of a WT Halocynthia roretzi mid gastrula stage embryo probed for ADP/ATP translocase expression.
Staining is observed in the whole embryo.
Original comment: Prior to the neurula stage, the expression in the germline could not be detected because of high background of staining due to the maternal mRNA.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) whole embryo -

H.roretzi, Stage 13 (late gastrula)

Read more…

Vegetal view of a WT Halocynthia roretzi late gastrula stage embryo probed for ADP/ATP translocase expression.
Staining is observed in the whole embryo.
Original comment: Prior to the neurula stage, the expression in the germline could not be detected because of high background of staining due to the maternal mRNA.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) whole embryo -

Vegetal view of a WT Halocynthia roretzi neural plate stage embryo probed for ADP/ATP translocase expression.
Staining is observed in the whole embryo.
Original comment: Prior to the neurula stage, the expression in the germline could not be detected because of high background of staining due to the maternal mRNA.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) whole embryo -

H.roretzi, Stage 13 (late gastrula)

Read more…

Vegetal view of a WT Halocynthia roretzi late gastrula stage embryo probed for ADP/ATP translocase expression.
Staining is observed in the whole embryo.
Original comment: Prior to the neurula stage, the expression in the germline could not be detected because of high background of staining due to the maternal mRNA.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) whole embryo -

Vegetal view of a WT Halocynthia roretzi neural plate stage embryo probed for ADP/ATP translocase expression.
Staining is observed in the whole embryo.
Original comment: Prior to the neurula stage, the expression in the germline could not be detected because of high background of staining due to the maternal mRNA.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) whole embryo -

H.roretzi, Stage 14 (early neurula)

Read more…

Vegetal view of a WT Halocynthia roretzi early neurula stage embryo probed for ADP/ATP translocase expression.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells (arrowheads).
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signals were visible in the germline.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 15 (mid neurula)

Read more…

Vegetal view of a WT Halocynthia roretzi mid neurula stage embryo probed for ADP/ATP translocase expression.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells (arrowheads).
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signals were visible in the germline.
Upper left panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 16 (late neurula)

Read more…

Vegetal view of a control Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and treated with DMSO.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a WT Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and injected with a control morpholino.
Original comment: Arrow indicates the ADP/ATP translocase signal in the germline.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 16 (late neurula)

Read more…

Vegetal view of a control Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and treated with DMSO.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a WT Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and injected with a control morpholino.
Original comment: Arrow indicates the ADP/ATP translocase signal in the germline.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 16 (late neurula)

Read more…

Vegetal view of a control Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and treated with DMSO.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a WT Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression.
Original comment: Arrow indicates germline expression and arrowheads somatic, possibly mesenchymal, expression.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Vegetal view of a Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and injected with a control morpholino.
Original comment: Arrow indicates the ADP/ATP translocase signal in the germline.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 16 (late neurula)

Read more…

Vegetal view of a Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and injected with morpholino oligos against Zf-1 and control oligos.
Weak expression is observed in the germline (arow).
Original comment: We found that the ADP/ATP translocase expression was significantly lower than that in control embryos when we injected them with MO against Zf-1.
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 16 (late neurula)

Read more…

Vegetal view of a Halocynthia roretzi late neurula stage embryo probed for ADP/ATP translocase expression and injected with morpholino oligos against Zf-1 and Pem.
Expression is observed in the germline (arrow).
Original comment: Co-injection of Pem MO together with Zf-1 MO restored ADP/ATP translocase expression in the germline (disrupted by Zf-1 MO alone).
Smaller image in the corner is an entire view of the embryo. Digits indicate the proportion of embryos with germline expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 20 (early tailbud II)

Read more…

Lateral view of a WT Halocynthia roretzi early tailbud stage embryo probed for ADP/ATP translocase expression.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells (arrowhead).
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

Lateral view of a control Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression and treated with DMSO.
ADP/ATP translocase is expressed in the germline and in the head (epidermis and mesenchyme).
Red arrows indicate positive ADP/ATP translocase expression near the tip of the tail (light blue bar).
The numbers represent the proportion of embryos without ADP/ATP translocase expression in the germline.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) head epidermis - mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

Lateral view of a Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression, injected with morpholinos against Pem and treated with DMSO.
ADP/ATP translocase is expressed in the mesenchyme.
Original comment: Embryos developed no ADP/ATP translocase expression in germline cells located at the tip of the tail when Pem was knocked down (56.3%, n ​= ​16; light blue bar).
The numbers represent the proportion of embryos without ADP/ATP translocase expression in the germline.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

Lateral view of a Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression, injected with morpholinos against Pem and treated with GSK126, a selective inhibitor of EZH2 used to inhibit H3K27me3.
ADP/ATP translocase is expressed in the mesenchyme and head epidermis.
Original comment: Embryos developed no ADP/ATP translocase expression in germline cells located at the tip of the tail when Pem and H3K27me3 levels were downregulated (44%, n ​= ​25; light blue bar).
The numbers represent the proportion of embryos without ADP/ATP translocase expression in the germline.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) head epidermis - mesenchyme (B8.5 & B7.7 lines) -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

MAGEST annotation : epidermis, adhesive organ, A-line notochord, B-line notochord, mesenchyme.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) epidermis - Palps - primary muscle lineage - secondary notochord lineage (B8.6 line) -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

MAGEST annotation : epidermis, adhesive organ, brain, mesenchyme, postplasmic RNA.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) anterior sensory vesicle - endodermal strand - epidermis - Palps - posterior sensory vesicle -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

MAGEST annotation : adhesive organ, brain, nerve cord, mesenchyme.

Stained molecule Harore.CG.MTP2014.S50.g14944 (Harore.CG.MTP2014.S50.g14944)
Stained region(s) anterior sensory vesicle - Palps - posterior sensory vesicle - tail nerve cord -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

MAGEST annotation : no expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) No expression -

H.roretzi, Stage 21 (mid tailbud I)

Read more…

MAGEST annotation : no expression.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) No expression -

H.roretzi, Stage 22 (mid tailbud II)

Read more…

Lateral view of a WT Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression.
ADP/ATP translocase is expressed in germline cells (red arrowheads) and in mesenchyme (purple arrowhead).
Scale bar, 100 μm.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 22 (mid tailbud II)

Read more…

Vegetal views of Halocynthia roretzi embryos probed for ADP/ATP translocase expression, in which cell divisions were arrested from the 110-cell stage onwards by the treatment of Cytochalasin B. They were fixed at the 110-cell equivalent stage.
ADP/ATP translocase is expressed in germline cells (red arrowheads), B7.5 cells (light blue arrowheads) and in mesenchyme-lineage cells (purple arrowhead).
Original comments: The image G’ was captured in a deeper focal plane than G to show the germline cells in focus (red arrowheads in G’) located behind the other vegetal cells shown in G (e.g. B7.5 cells, light blue arrowheads).
In some embryos from a late spawning season, germline cells swapped positions with their sister B7.5 cells even without Pem MO injection (H).

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) anterior ventral primary muscle lineage (B7.5 line) - mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) - trunk ventral cells (B7.5 line) -

H.roretzi, Stage 22 (mid tailbud II)

Read more…

Lateral view of a WT Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells (arrowhead).
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Lateral view of a WT Halocynthia roretzi early tailbud stage embryo probed for ADP/ATP translocase expression and injected with control LacZ mRNA.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells.
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 22 (mid tailbud II)

Read more…

Lateral view of a WT Halocynthia roretzi mid tailbud stage embryo probed for ADP/ATP translocase expression.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells (arrowhead).
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

Lateral view of a WT Halocynthia roretzi early tailbud stage embryo probed for ADP/ATP translocase expression and injected with control LacZ mRNA.
Original comment: Staining is observed in the germline (arrow) and in somatic, possibly mesenchymal, cells.
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -

H.roretzi, Stage 22 (mid tailbud II)

Read more…

Lateral view of a WT Halocynthia roretzi early tailbud stage embryo probed for ADP/ATP translocase expression and injected with Pem mRNA.
Staining is observed in the mesenchyme and very weakly in the germline (arrow) and in somatic, possibly mesenchymal, cells.
Original comment: The ADP/ATP translocase expression was reduced compared with that in the LacZ mRNA-injected control embryos.
The digits in the bottom right corner indicate proportions of the embryos in which the ADP/ATP translocase signal was visible in the germline.
Upper panel shows the entire view of the embryo. Dotted square represents the cropped area.

Stained molecule Harore.CG.MTP2014.S737.g07016 (SLC25A4; SLC25A5; SLC25A6)
Stained region(s) mesenchyme (B8.5 & B7.7 lines) - posterior endodermal strand (B8.11 line) - posterior endodermal strand (B8.12 line) -