1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
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You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
3 results
Vegetal view of a control Halocynthia roretzi 110-cell stage embryo probed for Mesp expression and treated with DMSO.
Mesp is specifically expressed in B7.5 blastomer pair.
Yellow arrows indicate the absence of ectopic gene expression in germline cells.
The numbers represent the proportion of embryos showing ectopic gene expression in the germline.
| Stained molecule | Harore.CG.MTP2014.S97.g06901 (MESP1; PTF1A; TCF21) |
| Stained region(s) | B7.5 cell pair - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Mesp expression, injected with morpholinos against Pem and treated with GSK126, a selective inhibitor of EZH2 used to inhibit H3K27me3.
Mesp endogenous expression is lost in 46.2% of embryos without Pem and H3K27me3.
Original comment: Pem- and H3K27me3-deficient embryos showed no ectopic Mesp expression in germline cells (0%, n = 13; yellow arrows).
| Stained molecule | Harore.CG.MTP2014.S97.g06901 (MESP1; PTF1A; TCF21) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Brachyury expression, injected with morpholinos against Pem and treated with GSK126, a selective inhibitor of EZH2 used to inhibit H3K27me3.
Brachyury is expressed in B8.6, B8.5, A8.5, A8.6, A8.13 and A8.14 blastomer pairs, as in WT conditions, and ectopically in B7.5, B7.2 and B7.7.
Original comment: Embryos lacking Pem and H3K27me3 ectopically expressed Brachyury in B7.5 and other posterior vegetal cells, but not in germline cells (0%, n = 8; yellow arrows).
The numbers represent the proportion of embryos showing ectopic gene expression in the germline.
| Stained molecule | Harore.CG.MTP2014.S97.g06901 (MESP1; PTF1A; TCF21) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.5 cell pair - A8.6 cell pair - B7.2 cell pair - B7.5 cell pair - B7.7 cell pair - B8.5 cell pair - B8.6 cell pair - |
