1 result
OVERVIEW
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
15 results
Vegetal views with anterior up of WT Halocynthia roretzi 64-cell stage embryos probed for ZicN expression.
ZicN is expressed in A7.3, A7.4, A7.7, A7.8, B7.3 and B7.4 blastomere pairs.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A7.3 cell pair - A7.4 cell pair - A7.7 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - |
Vegetal view with anterior up of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with PEM mRNA.
Expression is lost.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | No expression - |
Vegetal view with anterior up of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with Macho-1 mRNA.
Expression is lost.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with a control MO.
ZicN is expressed in A7.3, A7.4, A7.7, A7.8, B7.4 and B7.3 blastomer pairs.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A7.3 cell pair - A7.4 cell pair - A7.7 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with Fgf9/16/20 MO.
ZicN is expressed in A7.3, A7.4, A7.7, A7.8, B7.4 and B7.3 blastomer pairs, as in WT conditions.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A7.3 cell pair - A7.4 cell pair - A7.7 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with FoxDa MO.
ZicN is expressed in B7.4 and B7.3 blastomer pairs, expression in A-line is lost.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | B7.3 cell pair - B7.4 cell pair - |
Vegetal view of a Halocynthia roretzi 64-cell stage embryo probed for ZicN expression and injected with FoxA MO.
ZicN expression is lost.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | No expression - |
Vegetal views with anterior up of WT Halocynthia roretzi 110-cell stage embryos probed for ZicN expression.
ZicN is expressed in A8.13, A8.14, A8.15, A8.16, A8.5, A8.6, A8.7, A8.8, B8.5, B8.6, B8.7 and B8.8 blastomere pairs.
Expression is observed in B8.5 (black or red arrowheads), but not in B7.7 (white arrowheads) mesenchyme-lineage cells.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal views with anterior up of Halocynthia roretzi 110-cell stage embryos probed for ZicN expression and injected with PEM MO.
ZicN is expressed in WT blastomeres (A8.13, A8.14, A8.15, A8.16, A8.5, A8.6, A8.7, A8.8, B8.5 (red or black arrowheads), B8.6, B8.7 and B8.8), and ectopic expression is observed in B7.7 mesenchyme-lineage cells (white arrowheads) and other cells in the posterior half of the vegetal hemisphere.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair - B line - |
Vegetal view with anterior up of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression and injected with PEM and beta-catenin MOs.
ZicN expression is lost.
Ectopic expression in B7.7 caused by PEM knockdown is eliminated by simultaneous knockdown of beta-catenin.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 110-cell stage embryo probed for ZicN expression.
ZicN is expressed in A8.5, A8.6, A8.7, A8.8, A8.13, A8.14, A8.15, A8.16, B8.5, B8.6, B8.7 and B8.8 blastomer pairs.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair - B8.5 cell pair - B8.6 cell pair - B8.7 cell pair - B8.8 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression and injected with Hr-FoxA mRNA.
ZicN is ectopically expressed in animal territories (arrowhead).
Original comment: Hr-FoxA, when over-expressed, was able to induce ectopic Hr-ZicN expression.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression and injected with Hr-FoxDa mRNA.
ZicN is ectopically expressed in animal territories (arrowhead).
Original comment: Injection of Hr-FoxDa RNA resulted in ectopic expression of Hr-ZicN.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression, injected with Hr-FoxDa mRNA and treated with DMSO after fertilization (control).
ZicN is ectopically expressed in animal territories.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression and injected with Hr-FoxDa mRNA.
ZicN is ectopically expressed in animal territories (arrowhead).
Original comment: Injection of Hr-FoxDa RNA resulted in ectopic expression of Hr-ZicN.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression, injected with Hr-FoxDa mRNA and treated with DMSO after fertilization (control).
ZicN is ectopically expressed in animal territories.
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for ZicN expression, injected with Hr-FoxDa mRNA and treated with the MEK inhibitor U0126 after fertilization.
ZicN is ectopically expressed in animal territories.
Original comment: the treatment with UO126 did not affect the level of ectopic Hr-ZicN expression induced by Hr-FoxA (100% positive, n = 10).
| Stained molecule | Harore.CG.MTP2014.S22.g05991 (ZIC3; ZIC4; ZIC5) |
| Stained region(s) | a line - b line - |
