1 result
OVERVIEW
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OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
20 results
Animal (c) and vegetal (e) views of a Halocynthia roretzi 16-cell stage embryo probed for Otx expression and injected with control YFP RNA.
Otx is not yet expressed at this stage.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | No expression - |
Animal (d) and vegetal (f) views of a Halocynthia roretzi 16-cell stage embryo probed for Otx expression and injected with FGF9/16/20 mRNA .
Otx is ectopically expressed in all the cells, with the exception of the B5.2 blastomere pair.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - a5.3 cell pair - a5.4 cell pair - a line - A line - B5.1 cell pair - b5.3 cell pair - b5.4 cell pair - b line - |
Animal (a) and vegetal (b) views of a WT Halocynthia roretzi 32-cell stage embryo probed for Otx expression and injected with a control morpholino oligo.
Otx is expressed in B6.4 (yellow arrowhead), a6.5 and b6.5 (black arrowhead), B6.1 (red arrowhead) and weakly in a6.7 (orange arrowhead) blastomeres.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | a6.5 cell pair - a6.7 cell pair - B6.1 cell pair - B6.4 cell pair - b6.5 cell pair - |
Animal (c) and vegetal (d) views of a WT Halocynthia roretzi 32-cell stage embryo probed for Otx expression and injected with morpholino oligos against FGF9/16/20.
Otx is expressed in B6.42 (yellow arrowhead) and B6.1 (red arrowhead) blastomeres.
Original comment: Although the expression in cells of the animal hemisphere (a6.5; b6.5 and a6.7) and the B6.4 blastomere pair was eliminated in the absence of the FGF signal, the expression was still observed in the B6.1 blastomere pair and was newly detected in the B6.2 blastomere pair.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - |
An image of a 110-cell stage Halocynthia roretzi embryo stained with Otx in situ hybridization. Expression is observed in the brain precursors (white circles) and endoderm cells. Animal views with anterior to the top.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.25 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.7 cell pair - B8.5 cell pair - |
110-cell stage Halocynthia roretzi embryo probed for Otx expression and injected withPEM MO. Ectopic expression in cells at the periphery of the animal hemisphere is observed (black arrow heads). Animal (left) and posterior-vegetal (right) views. Anterior is up with normal expression in brain precursors indicated by white circles (left).
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - a8.25 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair - B7.7 cell pair - B8.5 cell pair - b line - |
Animal (e) and vegetal (f) views of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with a control morpholino oligo.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm in the vegetal hemisphere.
No consistent expression was detected in a8.25
blastomeres (orange arrowheads).
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads), and in the endoderm.
Scale bar, 100 μm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.1 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.2 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal (G) and vegetal (H) views of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with morpholino oligos against FGF9/16/20.
Otx is expressed in the endoderm.
Original comment: Expression in presumptive endoderm cells was independent of Hr-FGF9/16/20 signaling (100% unaffected, n=11), that in the presumptive brain
and palp cells was eliminated by the MO injection.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal (e) and vegetal (f) views of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with a control morpholino oligo.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm in the vegetal hemisphere.
No consistent expression was detected in a8.25
blastomeres (orange arrowheads).
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads), and in the endoderm.
Scale bar, 100 μm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.1 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.2 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal (e) and vegetal (f) views of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with a control morpholino oligo.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm in the vegetal hemisphere.
No consistent expression was detected in a8.25
blastomeres (orange arrowheads).
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads), and in the endoderm.
Scale bar, 100 μm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.1 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.2 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal (e) and vegetal (f) views of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with a control morpholino oligo.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm in the vegetal hemisphere.
No consistent expression was detected in a8.25
blastomeres (orange arrowheads).
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads), and in the endoderm.
Scale bar, 100 μm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.1 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with control MO in left A5.2 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 MO in left A5.1 at the 16-cell stage.
Otx expression in the presumptive brain and palp cells of the animal hemisphere is lost (white arrowheads on injected side). Endoderm expression seems unchanged.
The diagram is in vegetal view (anterior down), injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 MO in left A5.2 at the 16-cell stage.
Otx expression in the presumptive brain and palp cells of the animal hemisphere is strongly reduced (78.6% reduced, n=14, white arrowheads on injected side). Endoderm expression seems unchanged.
The diagram is in vegetal view (anterior down), injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.19 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a WT Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 MO in left B5.1 at the 16-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm, as in WT conditions.
The diagram is in vegetal view (anterior down), injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Fgf9/16/20 MO in left A6.1 at the 24-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm, as in WT conditions.
The diagram is in vegetal view (anterior down), injected blastomere is in red and brain precursors in blue.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 MO in left A6.2 at the 24-cell stage.
Otx expression in the presumptive brain and palp cells of the animal hemisphere is reduced ((60% reduced, n=10 -white arrowheads on injected side). Endoderm expression seems unchanged.
The diagram is in vegetal view (anterior down), injected blastomere is in red and brain precursors in blue.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.18 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Fgf9/16/20 MO in left A6.4 at the 24-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
The diagram is in vegetal view (anterior down), injected blastomere is in red and brain precursors in blue.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Fgf9/16/20 MO in left A6.2 at the 32-cell stage.
Otx is expressed in the presumptive brain and palp cells
in the animal hemisphere (a8.17, a8.18, a8.19 and a8.20 black arrowheads) and in the endoderm.
Original comment: Injection of the A6.2 blastomere at the 32-cell stage did not cause any reduction in the expression.
The diagram is in vegetal view (anterior down), injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - a8.17 cell pair - a8.18 cell pair - a8.19 cell pair - a8.20 cell pair - B7.1 cell pair - B7.2 cell pair - |
Animal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 mRNA in left b5.4 at the 16-cell stage.
Most of the cells in the animal hemisphere expressed Hr-Otx upon over-expression.
Scale bar, 100 μm.
The diagram is in animal view, injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | a line - b line - |
Vegetal view of a Halocynthia roretzi 110-cell stage embryo probed for Otx expression and injected with Hr-Fgf9/16/20 mRNA in left B5.1 at the 16-cell stage.
Otx is expressed in the endoderm and in b8.17, b8.18, and b8.21 blastomers.
Original comment: Ectopic Hr-Otx expression was observed in cells in the animal hemisphere (b8.17, b8.18, and b8.21 arrowheads). Note that the b8.17 and b8.18 blastomeres are descendants of the b6.5 blastomere, in which Hr-Otx is transiently expressed at the 32-cell stage.
The diagram is in vegetal view, injected blastomere is in red.
| Stained molecule | Harore.CG.MTP2014.S157.g05829 (CRX; OTX1; OTX2) |
| Stained region(s) | A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - B7.1 cell pair - B7.2 cell pair - b8.17 cell pair - b8.18 cell pair - b8.21 cell pair - |
