1 result
OVERVIEW
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
13 results
Vegetal view, anterior to the left of a 4-cell stage Halocynthia roretzi embryo probed for Not (brown) and PEM (red) expressions
Not is expressed in the A-line
Original annotation:Zygotic transcription is detected in the somatic, but not in the germline lineage. PEM mRNA is stained red for identification of germline cells.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A line - |
Vegetal view, anterior to the left of a 4-cell stage Halocynthia roretzi embryo probed for Not (brown) and PEM (red) expressions
PEM is expressed in the B-line
Original annotation: PEM mRNA is stained red for identification of germline cells.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B line - |
Vegetal view with anterior to the left of a Halocynthia roretzi 4-cell stage embryo probed for Not (brown) and PEM (red) expressions, injected with morpholinos against PEM.
Not expression is observed in all the cells.
Original annotation:Ectopic zygotic transcription in PEM knockdown embryos is marked with yellow arrowheads.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | whole embryo - |
Vegetal view with anterior left of a Halocynthia roretzi 4-cell stage embryo probed for Not (brown) and PEM (red) expressions, and injected with PEM MO.
PEM is expressed in the B-line
Original annotation: PEM mRNA is stained red for identification of germline cells.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B line - |
Vegetal view with anterior to the left of a Halocynthia roretzi 4-cell stage embryo probed for Not (brown) and PEM (red) expressions, and injected with PEM mRNA.
Original annotation: Somatic expression disappears in PEM mRNA injected embryos.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | No expression - |
Vegetal view with anterior to the left of a Halocynthia roretzi 4-cell stage embryo probed for Not (brown) and PEM (red) expressions, and injected with PEM mRNA.
No expression can be observed.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | No expression - |
Lateral view with anterior to the left of a WT Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions.
Not is expressed in a, A and b lines.
Original annotation: Not zygotic transcription is detected in the somatic, but not in the germline lineage.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | a line - A line - b line - |
Lateral view with anterior to the left of a WT Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions.
PEM is expressed in the B4.1 cell pair.
Original annotation: PEM mRNA is stained red for identification of germline cells.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B4.1 cell pair - |
Lateral view with anterior to the left of a Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with morpholinos against PEM.
Not expression is ubiquitously observed.
Original annotation: Not ectopic zygotic transcription in PEM knockdown embryos is marked with yellow arrowheads.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | whole embryo - |
Lateral view with anterior to the left of a Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with morpholinos against PEM.
PEM is expressed in the B line (germline).
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B4.1 cell pair - |
Lateral view with anterior to the left of a Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM mRNA.
Not expression is lost.
Original annotation: Somatic expression disappears in PEM mRNA injected embryos.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | No expression - |
Lateral view with anterior to the left of a Halocynthia roretzi 8-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM mRNA.
PEM expression is lost.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | No expression - |
Vegetal view with anterior to the top of a WT Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions.
Not is expressed in a, A and b lines and in B5.1.
Original annotation: Not zygotic transcription is detected in the somatic, but not in the germline lineage.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | a line - A line - B5.1 cell pair - b line - |
Vegetal view with anterior to the top of a WT Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions.
PEM is expressed in the B5.2 cell pair.
Original annotation: PEM mRNA is stained red for identification of germline cells.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B5.2 cell pair - |
Vegetal view with anterior to the top of a Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM MO.
Not is ubiquitously expressed.
Original annotation: Not ectopic zygotic transcription in PEM knockdown embryos is marked with yellow arrowheads.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | whole embryo - |
Vegetal view with anterior to the top of a Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM MO.
PEM is expressed in B5.2 cell pair (germline cells).
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | B5.2 cell pair - |
Vegetal view with anterior to the top of a Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM mRNA.
Not somatic expression is lost.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | No expression - |
Vegetal view with anterior to the top of a Halocynthia roretzi 16-cell stage embryo probed for Not (dark blue) and PEM (red) expressions, and injected with PEM mRNA.
PEM expression is lost.
| Stained molecule | Harore.CG.MTP2014.S480.g14149 (Harore.CG.MTP2014.S480.g14149) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for Not expression.
Zygotic transcription is detected in the somatic, but not in the germline lineage. Not mRNA is stained Dark blue or brown. PEM mRNA is stained red for identification of germline cells. Anterior is to the top.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - a line - B6.2 cell pair - b line - |
Halocynthia roretzi 32-cell stage embryo probed for FoxA expression and injected with 300pg of PEM MO.
Ectopic zygotic transcription in PEM knockdown embryos is marked with yellow arrowheads.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - B6.2 cell pair - B6.3 cell pair - B6.4 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for Not expression and injected with 100pg of PEM MO.
Not remains expressed in its WT territories.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - a line - B6.2 cell pair - b line - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for Not expression and injected with PEM mRNA.
.Somatic expression disappears in PEM mRNA injected embryos.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | No expression - |
Halocynthia roretzi 32-cell stage embryo probed for FoxA expression and injected with 300pg of PEM MO.
Ectopic zygotic transcription in PEM knockdown embryos is marked with yellow arrowheads.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - B6.2 cell pair - B6.3 cell pair - B6.4 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for Not expression and injected with 100pg of PEM MO.
Not remains expressed in its WT territories.
| Stained molecule | Harore.CG.MTP2014.S4.g04053 (EMX1; MEOX1; VAX1) |
| Stained region(s) | A6.2 cell pair - A6.4 cell pair - a line - B6.2 cell pair - b line - |
