1 result
OVERVIEW
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
35 results
WT Halocynthia roretzi fertilized egg probed for FoxDa expression.
FoxDa is not expressed at this stage.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
WT Halocynthia roretzi 4-cell stage embryo probed for FoxDa expression.
FoxDa is not yet expressed at this stage.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal views with anterior up of WT Halocynthia roretzi early 16-cell stage embryos probed for FoxDa expression.
FoxDa is expressed in A5.1, A5.2 and B5.1 blastomere pairs.*
Scale bar = 100 μm
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - |
Vegetal views with anterior up of Halocynthia roretzi early 16-cell stage embryos probed for FoxDa expression and injected with PEM MO.
FoxDa is expressed in A5.1, A5.2 and B5.1 blastomere pairs (as in WT embryos) and FoxDa is ectopically expressed in B5.2 blastomeres (arrowheads).
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view with anterior up of a Halocynthia roretzi early 16-cell stage embryo probed for FoxDa expression and injected with PEM and beta-catenin MOs.
FoxDa expression is lost.
Ectopic expression in B5.2 caused by PEM knockdown is eliminated by simultaneous knockdown of beta-catenin.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal views with anterior up of a Halocynthia roretzi early 16-cell stage embryo probed for FoxDa expression and injected with PEM mRNA.
Expression is absent on the injected side.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi 16-cell stage embryo probed for FoxDa expression.
FoxDa is expressed in A5.1, A5.2 and B5.1 blastomeres (arrowheads).
Original comment: HrFoxDa is expressed in cells that are fated to become both notochord and endoderm at the 16-cell stage.
Diagram : the vegetal hemisphere of the 16-cell embryo illustrating in orange the expression of Hr-FoxDa.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - |
Vegetal views of WT Halocynthia roretzi 16-cell stage embryos probed for FoxD.a expression and injected with a control morpholino oligo.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but is never expressed in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrowheads or arrows).
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 24% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1500 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 95% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1 and control LacZ mRNA.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 29% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 24% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1500 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 95% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1 and control LacZ mRNA.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 29% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 24% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1500 pg of morpholino oligos against Popk-1.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 95% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1 and control LacZ mRNA.
FoxD.a is expressed in A5.1, A5.2 and B5.1 blastomer pairs, but also in B5.2 blastomeres, the germline cells of the 16-cell stage embryos (black arrows), in 29% of the embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - B5.1 cell pair - B5.2 cell pair - |
Vegetal view of a Halocynthia roretzi 16-cell stage embryo probed for FoxD.a expression and injected with 1000 pg of morpholino oligos against Popk-1 and Pem mRNA.
FoxD.a expression is lost.
Original comment: Co-injection of Popk-1 MO and Pem mRNA abolished the ectopic signal in the germline (arroxs) as well as signals in the somatic cells.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression.
Zygotic transcription is detected in the somatic, but not in the germline lineage. FoxDa mRNA is stained Dark blue or brown. PEM mRNA is stained red for identification of germline cells (left). Anterior is to the top.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - B6.1 cell pair - B6.2 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with 300pg of PEM MO.
FoxDa is expressed in the A-line and B6.1 and B6.2 blastomere pairs.
No ectopic expression is observed in PEM knockdown embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - B6.1 cell pair - B6.2 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with 100pg of PEM MO.
FoxDa is expressed in the A-line and B6.1 and B6.2 blastomere pairs.
No ectopic expression is observed in PEM knockdown embryos.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - A line - B6.1 cell pair - B6.2 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM mRNA.
Somatic expression disappears in full length PEM mRNA injected embryos. 50 pg injected (left; middle) and 10 pg injected (right).
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM delta 11-89 mRNA.
No somatic expression after PEM delta 11-89 mRNA injection indicates that PEM's transcriptional repression activity is retained without 11-89.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM delta 90-171 mRNA. No somatic expression after PEM delta 90-171 mRNA injection indicates that PEM's transcriptional repression activity is retained without 90-171.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | B6.4 cell pair - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM delta 172-257 mRNA.
No somatic expression after PEM delta 172-257 mRNA injection indicates that PEM's transcriptional repression activity is retained without 172-257.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM delta 258-341 mRNA.
No somatic expression after PEM delta 258-341 mRNA injection indicates that PEM's transcriptional repression activity is retained without 258-341.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Halocynthia roretzi 32-cell stage embryo probed for FoxDa expression and injected with PEM delta 342-426 mRNA.
No change in somatic expression after PEM delta 342-426 mRNA injection indicates that PEM's transcriptional repression activity is lost without 342-426.
| Stained molecule | Harore.CG.MTP2014.S128.g04021 (FOXD1; FOXD2; FOXD4) |
| Stained region(s) | A6.1 cell pair - A6.2 cell pair - A6.3 cell pair - A6.4 cell pair - B6.1 cell pair - B6.2 cell pair - |
