1 result
OVERVIEW
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Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
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| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | H.roretzi, Stage 0 (Unfertilized egg) | |
| H.roretzi, Stage 8 (64-cell) | ||
| H.roretzi, Stage 12 (mid gastrula) | ||
| H.roretzi, Stage 15 (mid neurula) | ||
| H.roretzi, Stage 21 (mid tailbud I) | ||
| H.roretzi, Stage 26 (hatching larva) | ||
7 results
Vegetal view of a WT Halocynthia roretzi early gastrula stage embryo probed for Twist-like expression.
Twist-like is not yet expressed at this stage.
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | No expression - |
Vegetal view of a WT Halocynthia roretzi mid gastrula stage embryo probed for Twist-like expression.
Twist-like expression was first detected in mesenchyme cells at the mid-gastrula stage (black arrowheads).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Lateral (D) and ventral (E) views of a WT Halocynthia roretzi early neurula stage embryo probed for Twist-like expression.
Twist-like is specifically expressed in mesenchyme cells.
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Vegetal view of a WT Halocynthia roretzi mid tailbud stage embryo probed for Twist-like expression.
Twist-like is mainly expressed in the mesenchyme, and slight expression is also observed in trunk lateral cells (white arrowhead).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - trunk lateral cells (A7.6 line) - |
Expression of Twist-like in Halocynthia roretzi embryo whose cell division was arrested during the period from the 110-cell stage to the mid tailbud stage. This embryo was injected with a control MO. Vegetal view.
Original comment: Twist-like expression was strongly detected at the equivalent of the mid-tailbud stage in anterior mesenchyme lineage cells, but only weakly in posterior mesenchyme lineage cells. No embryo shows ectopic expression (0/9).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Expression of Twist-like in a WT Halocynthia roretzi embryo whose cell division was arrested during the period from the 110-cell stage to the mid tailbud stage. Vegetal view.
Original comment: Twist-like expression was strongly detected at the equivalent of the mid-tailbud stage in anterior mesenchyme lineage cells, but only weakly in posterior mesenchyme lineage cells. No embryo shows ectopic expression (0/16).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Expression of Twist-like in Halocynthia roretzi embryo whose cell division was arrested during the period from the 110-cell stage to the mid tailbud stage. This embryo was injected with a control MO. Vegetal view.
Original comment: Twist-like expression was strongly detected at the equivalent of the mid-tailbud stage in anterior mesenchyme lineage cells, but only weakly in posterior mesenchyme lineage cells. No embryo shows ectopic expression (0/9).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Expression of Twist-like in a WT Halocynthia roretzi embryo whose cell division was arrested during the period from the 110-cell stage to the mid tailbud stage. Vegetal view.
Original comment: Twist-like expression was strongly detected at the equivalent of the mid-tailbud stage in anterior mesenchyme lineage cells, but only weakly in posterior mesenchyme lineage cells. No embryo shows ectopic expression (0/16).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - |
Expression of Twist-like in Halocynthia roretzi embryo whose cell division was arrested during the period from the 110-cell stage to the mid tailbud stage. This embryo was injected with Tbx6 MO. Vegetal view.
Original comment: Twist-like is expressed in mesenchyme cells and ectopic expression is pobserved in cells located at the peripheral posterior region of the embryo (white arrowheads).
| Stained molecule | Harore.CG.MTP2014.S124.g01722 (Harore.CG.MTP2014.S124.g01722) |
| Stained region(s) | mesenchyme (B8.5 & B7.7 lines) - tail muscles - |
