Aniseed - Gene Card

EST count

No result

RNA-Seq transcriptome profiles (by Experiment)

1 result

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

Relative Log Expression RLE

Be careful, RLE and 2^RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34137

Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).

Assay platform	Illumina HiSeq 2000
Layout	Paired-end
Stranded	Yes
Read length	49
Normalized data types	FPKM RLE
Wild type - Replica 1
	*Territories*	whole embryo -

	*Biomaterial(s)*	H.roretzi, Stage 0 (Unfertilized egg)
		H.roretzi, Stage 8 (64-cell)
		H.roretzi, Stage 12 (mid gastrula)
		H.roretzi, Stage 21 (mid tailbud I)
		H.roretzi, Stage 26 (hatching larva)

Wild type - Replica 2
	*Territories*	whole embryo -

	*Biomaterial(s)*	H.roretzi, Stage 0 (Unfertilized egg)
		H.roretzi, Stage 8 (64-cell)
		H.roretzi, Stage 12 (mid gastrula)
		H.roretzi, Stage 15 (mid neurula)
		H.roretzi, Stage 21 (mid tailbud I)
		H.roretzi, Stage 26 (hatching larva)

In Situ Experiment Data

3 results

Experiment data ( results)

H.roretzi, Stage 4 (8-cell)

MAGEST annotation : a4.2, b4.2, A4.1.

Stained molecule	Harore.CG.MTP2014.S221.g00015 (Harore.CG.MTP2014.S221.g00015)
Stained region(s)	A4.1 cell pair - a4.2 cell pair - b4.2 cell pair -

H.roretzi, Stage 10 (110-cell)

MAGEST annotation : no expression.

Stained molecule	Harore.CG.MTP2014.S221.g00015 (Harore.CG.MTP2014.S221.g00015)
Stained region(s)	No expression -

H.roretzi, Stage 21 (mid tailbud I)

MAGEST annotation : no expression.

Stained molecule	Harore.CG.MTP2014.S221.g00015 (Harore.CG.MTP2014.S221.g00015)
Stained region(s)	No expression -