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  1. EST 'AL666842'

EST count

27 results

Library Name Percentage Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal 0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library 0 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult 1 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo 0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg 2 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva 1 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo 5 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library 0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library 0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis 0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library 0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva 0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells 0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle 0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo 1 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula 2 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad 0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex 0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart 0 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland 0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal 1 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal 1 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal 0 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal 0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile 0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) 0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) 1 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

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Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

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Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform Ion Torrent PGM
Layout Single-end
Stranded Yes
Read length 20-365
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)
Mutant
Deregulated molecule(s) KH2012:KH.C8.890 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

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The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform Illumina HiSeq 2500
Layout Single-end
Stranded Yes
Read length 101
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 1
Deregulated molecule(s) KH2012:KH.C4.125 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 2
Deregulated molecule(s) KH2012:KH.C2.573 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

105 results

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: No signal was detected.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: No zygotic signal was detected.

Aniseed annotation:
No picture available for this stage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: No zygotic signal was detected.

Aniseed annotation:
No picture available for this stage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

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A 32 cells stage wild type embryo probed for Delta like expression.

Original annotation:
Expression was detected in whole embryo.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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No picture available in the article.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for Delta-like expression and injected with ephrin-Ad MO.

Original annotation:
Delta-like is the most resistant to MO inhibition with only 40% loss of expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage wild type embryo probed for Ci-Delta2 expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for Ci-delta2 expression in which Ci-FoxAa has been blocked by MO injection. Control picture: sibling embryo shows the wild type expression pattern of the gene.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) a7.10 cell pair - a7.9 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Original annotation:
Gene name: Delta-like.
Expression pattern: A7.6 A7.8 b7.9 b7.10

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Animal view of a 64-cell stage embryo probed for Delta-like expression following egg micro-injection of a FoxA-a MO. Delta-like was ectopically expressed in a-line neural cells, the expression in TLCs and b-line neural cells were unaffected.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - a line - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: A7.6 A7.8 b7.9 b7.10

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal and lateral view of a 64-cell stage wild type embryo probed for Ci-Delta2 expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for Ci-Delta2 expression in which Nodal signalling has been blocked with SB431542. No signal can be identified.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for Ci-Delta2 expression in which Nodal signalling has been inhibited with Nodal-Mo. No signal can be identified.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage wild type embryo probed for Ci-delta2 expression. GFP mRNA was injected into A4.1 blastomere on the right hand side.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for Ci-Delta2 expression in which Nodal signalling has been blocked with tALK4/5/7 mRNA. mRNA were injected into A4.1 on the right hand side at 8-cell stage and then analysed for Ci-delta2 expression at 64-cell stage (left side is used as a control). There is no staining on the right side compared to the control side.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Lateral view with the vegetal pole to the left of a 64-cell stage embryo probed for Ci-Delta2 expression.

Original Annotation : Ci-Delta2 is expressed in A7.6, b7.10 and b7.9 pair cells, with weak expression in A7.8 cell pair.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A7.8 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal pole view of a 64-cell stage embryo probed for Ci-Delta2 expression following b5.3 ablation on the right side. The Nodal source of signal was sufficient to abolish Ci-Delta2 expression in A7.6 and A7.8 on the treated side.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 9 (76-cell)

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Lateral view with the vegetal pole to the left of a 76-cell stage embryo probed for Ci-Delta2 expression.

Original Annotation : Ci-Delta2 is expressed in A8.15 , A8.16, A7.6, b7.9 and b7.10 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.6 cell pair - A8.15 cell pair - A8.16 cell pair - b7.10 cell pair - b7.9 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: A7.6 b8.17 b8.18 b8.19 b8.20 A8.15 A8.16.

Aniseed annotation : A staining may be detected in the A7.5 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a WT 64-cell stage embryo probed for Ci-delta2.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 64-cell stage embryo probed for Ci-delta2 expression following electroporation with pSP1.72-FOG::Ci-FoxA-a overexpression construct. Control: sibling embryo shows the wild type expression pattern of the gene. Expression is lost after FoxA-a electroporation.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal, and lateral views of a 110-cell stage wild-type embryo probed for Ci-Delta2 expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal and lateral views of a 110-cell stage embryo probed for Ci-Delta2 expression following egg micro-injection of a mRNA Nodal. Ci-Delta2 was expressed in up to all eight A-line neural cells in 59% of cases. Control sibling embryo shows the wild type pattern of the gene.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Ci-Delta2 expression in which Nodal signalling has been blocked by incubation into the SB431542 inhibitor of Alk4/5/7. The expression was lost in b-line, A8.15 and A8.16. Control sibling embryo shows the wild type pattern of the gene.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Ci-Delta2 expression in which Nodal signalling has been blocked by injection of a Nodal MO. The expression was lost in b-line, A8.15 and A8.16. Control sibling embryo shows the wild type pattern of the gene.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Ci-Snail expression in which Nodal signalling has been blocked by injection of mRNA for a dominat negative version of the Alk4/5/7 type I nodal receptor. The expression was lost in b-line, A8.15 and A8.16. Control sibling embryo shows the wild-type pattern of the gene.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Original annotation:
Gene name: Delta-like.
Expression pattern: A7.6 b8.17 b8.18 b8.19 b8.20 A8.15 A8.16.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like expression following egg micro-injection of a Neurogenin MO. Delta-like is down-regulated in A7.6.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like expression following egg micro-injection of a Nodal MO.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like expression following egg micro-injection of a ZicL MO.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like expression following egg micro-injection of a FoxD-a/b MO. Delta-like expresssion was down-regulated in A-line nerve cord cells and not affected in b-line.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo showing expression of Delta-like in A7.6, b7.9 and b7.10 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo showing expression of Delta-like. Embryos were treated with U0126 at the late 32-cell stage.

Original annotation
A7.6 group genes are ectopically expressed in the anterior endoderm blastomeres upon MEK inhibition, whereas A7.6 expression is unaffected.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal views of 110-cell stage embryos co-injected with ephrin-Ad MO and torso-FGFR mRNA into 1-cell stage and probed for Delta-like.

Original annotation:
Delta-like expression was now repressed in the A7.6 blastomere in 80% of the embryos (arrows). Ectopic activation of Delta-like in b-line blastomeres is often observed (arrowheads).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b line -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like and after injection of FoxD::ephrin-Ad. This construct was used to drive Ci-ephrin-Ad throughout the vegetal hemisphere at the 16-cell stage (thanks to FoxD enhancer).

Original annotation:
Delta-like expression is observed in A7.6 blastomeres and in anterior endoderm.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.2 cell pair - A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal and lateral views of 110-cell stage embryos injected at the 1-cell stage with torso-FGFR mRNA and probed for delta.

Original annotation:
Delta-like expression was only sporadically reduced.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.19 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo probed for Delta-like following injection of FoxD::Nodal. This construct was used to drive Nodal throughout the vegetal hemisphere (thanks to FoxD enhancer).

Original Annotation: Delta-like sometimes displayed ectopic expression in the A-line neural precursors, indicating by arrowheads.

Aniseed Annotation: Ectopic expression of Hand-like is showing in A8.7 and A8.8 cells.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.7 cell pair - A8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo showing expression of Delta-like. Embryos were treated with U0126 at the 16-cell stage (picture and graph) and at the early 32-cell stage, late 32-cell stage and 64-cell stage (graph).

Original annotation:
These embryos often displayed normal expression in A7.6 cell pair but additional patterns were also detected, including expression in A7.6 along with ectopic expression in the endoderm, loss of A7.6 expression along with gain of expression in the endoderm, or complete loss of expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.1 cell pair - A7.2 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of 110-cell stage embryo injected with ephrin-Ad MO and treated with the MEK inhibitor U0126 at the 32-cell stage and probed for Delta-like expression.

Original annotation :
Delta-like is expressed in both the A7.6 blastomere and the anterior endoderm.

Aniseed annotation :
According to the picture, we can't determine if there is expression in b7.9 and b7.10 cell pairs (compare to the control).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo showing Ci-Delta-like expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo. The expression of Ci-delta-like was examined in embryos that expressed Ci-FoxD/Ci-nodal.

Original annotation:
The Ci-FoxD/Ci-nodal transgene mediated ectopic expression of Ci-delta-like in the ventral nerve cord lineage (red arrowheads).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo. The expression of Ci-delta-like was examined in SB431542-treated embryos.

Original annotation:
SB431542 induced repression of Ci-delta-like (blue arrowheads).

Aniseed annotation:
Very weak expression seems to be detected in A7.6 cell pair.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cell stage embryo. The expression of Ci-delta-like was examined in embryos that expressed Ci-FoxD/Ci-lefty.

Original annotation: The Ci-FoxD/Ci-lefty transgene mediated repression of Ci-delta-like (blue arrowheads).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal and lateral views of an early gastrula stage embryo probed for Ci-Delta2 expression.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal and lateral views of an early gastrula stage embryo probed for Ci-Delta2, following injection of a morpholino against Delta2 (Delta2-MO bis). Numbers indicate any expression over the total number of embryos analyzed.

Original Annotation :
Expression is not affected by the inhibition of Delta2/Notch signaling.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal and lateral views of an early gastrula stage embryo probed for Ci-Delta2, following injection of DAPT. Numbers indicate any expression over the total number of embryos analyzed.

Original Annotation :
Expression is not affected by the inhibition of Delta2/Notch signaling due to DAPT.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal pole view of embryos in which the b6-5 blastomere was ablated on the right side and probed for Ci-Delta2 expression at the 110-cel stage. Weak expression in some embryos may be due to some signal already having passed between b6.5 and the A-line neural lineage before ablation was carried out (20/36). Complete loss of expresion in the A-line is observed in the other embryos (16/36). Control sibling embryo shows the wild type pattern of expression of the gene. To facilitate data mining, the expression pattern is described as lost expression in the A-line when b6.5 derivatives are ablated.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2. In the panel, anterior ectoderm in white, posterior ectoderm in yellow, gene expression in blue and black arrowheads indicate b-line neural precursors.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after electroporation with the construct pFOG::FGF9/16/20 at the 16-cell stage. In the panel, gene expression is presented in blue. Expression was observed in the posterior ectoderm (b4.2 lineage or b-line ectoderm).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b line -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after electroporation with the construct pFOG::Lefty, a Nodal antagonist at the 16-cell stage. In the panel, posterior ectoderm is marked in yellow and anterior ectoderm in white. Expression was lost in the b-line neural lineage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after electroporation with the pFOG::Lefty (Nodal antagonist) and pFOG::FGF9/16/20 constructs at the 16-cell stage. In the panel, posterior ectoderm is marked in yellow and anterior ectoderm in white. Expression was lost in the b-line neural lineage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top (neural plate view with vegetal side to the left) of a 112-cell stage embryo (stage 10) stained with Delta2 after electroporation with the construct pFOG>Nodal at the 16-cell satge. In the panel, posterior ectoderme is marked in yellow and gene expression in blue.
Original annotation: There was no ectopic expression of Delta2 in the posterior (b-line) ectoderm. But ectopic expression of Delta2 was observed in the anterior neural tissue precursors (a6.5 lineage).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - a8.18 cell pair - a8.20 cell pair - A8.7 cell pair - A8.8 cell pair - b8.18 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after electroporation with the pFOG>Nodal and pFOG::FGF9/16/20 constructs at the 16-cell satge. In the panel, gene expression is marked in blue.
Original annotation: Induction of posterior neural tissue in anterior ectoderm.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) a line - b line -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view with anterior to the top of a 10-stage embryo stained with Delta2 afetr U0126 treatment at the 8-cell stage (MEK inhibitor). Black arrowhead indicates the b-line neural precursors. In In the panel, anterior ectoderm in white and posterior ectoderm in yellow. Expression of Delta2 was lost in the four daughter cells of the b6.5 lineage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 10-stage embryo stained with Delta2 after SB431542 (Nodal signaling pathway inhibitor) treatment at the 16-cell stage. In the panel, anterior ectoderm is marked in white and posterior ectoderm in yellow. Black arrowheads indicate b-line neural precursors. Delta2 expression was abolished in the four daughter cells of the b6.5 lineage.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after injection of a control morpholino. In the panel, anterior ectoderm in white, posterior ectoderm in yellow and gene expression in blue. Expression was observed in the b8.17, b8.18, b8.19 and b8.20 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after injection of a Otx morpholino (Otx MO). In the panel, anterior ectoderm in white, posterior ectoderm in yellow and gene expression in blue. Expression was lost in the b8.17, b8.18, b8.19 and b8.20 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view with anterior to the top of a 112-cell stage embryo (stage 10) stained with Delta2 after injection of a Otx overconstruct (pFOG-Otx. In the panel, anterior ectoderm in white, posterior ectoderm in yellow and gene expression in blue. Otx overexpression was sufficient to activate throughout the ectoderm.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) a line - b line -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view, anterior to the top, of a 112-cell stage embryo stained with Delta2.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Animal view, anterior to the top, of a 10-stage embryo stained with Delta2 after OtxHDenR injection, a dominant negative form of Otx, at the 16-cell stage. Expression was lost as indicated by the black arrow.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 11 (early gastrula)

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Animal view with anterior to the top of an early gastrula stage embryo stained with Delta2.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b8.17 cell pair - b8.18 cell pair - b8.19 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 11 (early gastrula)

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Animal view with anterior to the top of an early gastrula stage embryo stained with Delta2 after injection of pFOG::Lefty at the 16-cell stage. Expression was lost in the whole embryo.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 11 (early gastrula)

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Animal view with anterior to the top of an early gastrula stage embryo stained with Delta2 after overexpression of pFOG>Otx at the 16-cell stage. Ectopic expression was observed in the embryo.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 11 (early gastrula)

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Animal view with anterior to the top of an early gastrula stage embryo stained with Delta2 after overexpression of pFOG>Otx and pFOG::Lefty at the 16-cell stage. Ectopic expression was observed in the embryo.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 11 (early gastrula)

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Tilted view of an early gastrula stage embryo stained with Delta2 after overexpression of pFOG>Nodal at the 16-cell stage. Delta2 expressing cells are colored in blue and ectopic expression is circled in red.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A8.15 cell pair - A8.16 cell pair - a8.18 cell pair - a8.20 cell pair - A8.7 cell pair - A8.8 cell pair - b8.18 cell pair - b8.20 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

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Vegetal view of a late gastrula stage wild type embryo probed for Delta-like expression.

Original Annotation :
Delta-like was expressed in A9.32, b9.37 and b9.38 cell pairs.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A9.32 cell pair - b9.37 cell pair - b9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

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Vegetal view of a late gastrula stage embryo probed for Delta-like expression, following the injection of Neurogenin-MO.

Original annotation:
Expression in A9.32 cell pair was lost (white arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b9.37 cell pair - b9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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A late gastrula wild type embryo probed for Delta-like expression.

Original Annotation : Expression was detected in b and A line neural plate, A9.29, A9.30, A9.32, b9.33, b9.37 and b9.38.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A9.29 cell pair - A9.30 cell pair - A9.32 cell pair - b9.33 cell pair - b9.37 cell pair - b9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation: Gene name: Delta-like.
Expression pattern: nervous system.
Comment : decendant of b7.9, b7.10 and A9.32, a9.49.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A9.32 cell pair - a9.49 cell pair - b9.33 cell pair - b9.37 cell pair - b9.38 cell pair - dorsal midline anterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) - posterior most secondary muscle lineage (b8.17 line) -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Vegetal view of a WT Ciona robusta 6-row neural plate stage embryo probed for Delta expression and counterstained with DAPI.
Delta expression is observed in a9.50, a9.49, A9.32 and A9.31 blastomers.
In the drawing, Delta expression is indicated by black dots, with weaker expression represented by grey dots. The a-lineage neural plate cells that generate the CNS are coloured in red.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A9.32 cell pair - a9.49 cell pair - a9.50 cell pair - posterior secondary muscle lineage (A9.31 line) -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Vegetal view of a WT Ciona robusta 6-row neural plate stage embryo probed for Delta-like expression.
Delta-like expression is observed in a9.49 (column 3 of neural plate row III, pink arrowhead), A9.31 and A9.32 blastomers.
n=total number of embryos analysed.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) A9.32 cell pair - a9.49 cell pair - posterior secondary muscle lineage (A9.31 line) -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Vegetal view of a Ciona robusta 6-row neural plate stage embryo probed for Delta-like expression and injected with a morpholino against Nodal.
Delta-like expression is lost when Nodal is inhibited.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Vegetal view of a Ciona robusta 6-row neural plate stage embryo probed for Delta-like expression and treated with a pharmacological inhibitor of TGFβ type I receptors ALK4, ALK5 and ALK6 (SB431542) that inhibits Nodal activity.
Delta-like expression is lost when Nodal is inhibited.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Vegetal view of a Ciona robusta 6-row neural plate stage embryo probed for Delta-like expression and electroporated with pFOG>Nodal to drive expression of Nodal throughout the animal hemisphere from the 16-cell stage of development.
Delta-like expression is enlarged throughout the neural plate when Nodal is overexpressed. Strong Delta-like expression is observed in neural plate rows I, II and III.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neural plate - row III neural plate - row II neural plate - row I neural plate -

C. robusta formely Ciona int. type A, Stage 14 (early neurula)

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Dorsal view, anterior to the left, of a 14 stage embryo electroporated embryo with the « delta2-b6.5 line » enhancer and stained with X-gal.
Original annotation: Placed upstream of the minimal promoter of Fog and the reporter gene LacZ, this fragment drove transcription throughout b6.5 derivatives from the early gastrula stage.
Aniseed annotation: Expression was observed in the b9.33, b9.37, b9.38 cell pairs as well as in the dorsal midline anterior tail epidermis and in the posterior dorsal midline head epidermis.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) b9.33 cell pair - b9.37 cell pair - b9.38 cell pair - dorsal midline anterior tail epidermis - posterior dorsal midline head epidermis (b8.20 line) -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: nervous system.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

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Original annotation: no expression in ventral ectoderm. Lateral view of the embryo is shown.
Aniseed annotation: expression is observed in palps (a8.18 and a8.20 cell pairs), part of brain and nerve cord (A line neural plate).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) a10.69 cell pair - a10.70 cell pair - a10.77 cell pair - a10.78 cell pair - a9.36 cell pair - a9.40 cell pair - A line neural plate -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

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Original annotation: no expression in ventral ectoderm. Lateral view of the embryo is shown.
Aniseed annotation: expression is observed in palps, part of brain and nerve cord.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neck - neurohypophysis primordium - tail nerve cord - ventral VG ependymal precursors -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

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Dorsal view of the motor ganglion of a WT Ciona robusta initial tailbud embryo probed for Delta2 expression (red) and electroporated with FGF8/17/18>GFP reporter construct (green nuclei).
Original comment: Delta2 is normally expressed in A10.59 pair. Weaker/transient expression is sometimes seen in A10.60 (control embryo, left side), but it is never equal to that of A10.59.
The A9.30 lineage is visualized using the FGF8/17/18 reporter construct (green nuclei).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

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Dorsal view of the motor ganglion of a Ciona robusta initial tailbud embryo probed for Delta2 expression (red) and electroporated with FGF8/17/18>GFP reporter construct (green nuclei) and FGF8/17/18>Eph3ΔC overexpression construct.
Original comment: Conversion of A10.59 to A10.60 fate with FGF8/17/18>Eph3ΔC results in loss of Delta2 expression in A10.59 in electroporated side but not in unelectroporated side.
The A9.30 lineage is visualized using the FGF8/17/18 reporter construct (green nuclei).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: nervous system, Nerve cord, epidermis.
Comment : epidermis aroud the palp and in the tip of the tail.

Aniseed annotation :
A staining has also been detected in the nervous system.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) head epidermis - posterior tail epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Dorsal view of an early tailbud stage wild type embryo probed for Delta-like expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Delta-like was expressed in A11.118 and A11.117 cell pairs (posterior visceral ganglion).

Aniseed Annotation :
It seems that there is also expression in the anterior sensory vesicle.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Dorsal view of an early tailbud stage embryo probed for Delta like expression, following the injection of Neurogenin-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Expression was lost (white arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Dorsal view of an early tailbud stage embryo probed for Delta-like expression following the injection of Delta-like-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Ectopic expression was observed (black arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior sensory vesicle - neck - tail nerve cord - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Original annotation: expression in prospective ESNs (Epidermal Sensory Neurons)
Aniseed annotation: expression in palps, brain and tail nerve cord.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) dorsal anterior sensory vesicle - tail nerve cord - ventral posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Views of early tailbud stage showing reporter expression of the region upstream of delta2 in a part of trunk, epidermis around ESN and tail epidermis(dorsal midline and b region).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) dorsal midline head epidermis - siphon primordia - tail epidermis - trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of an early tailbud stage embryo stained with Delta2. Expression was observed in the tail epidermis.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) caudal epidermal neurone (CEN) precursors -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of the motor ganglion of a WT Ciona robusta early tailbud embryo probed for Delta2 expression (red) and electroporated with FGF8/17/18>GFP reporter construct (green nuclei).
Delta2 is normally expressed in A11.117 andA11.118 pairs (right arrowheads). Weaker expression is observed in A11.120 (left arrowhead).
The A9.30 lineage is visualized using the FGF8/17/18 reporter construct (green nuclei, diagram).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of the motor ganglion of a Ciona robusta early tailbud embryo probed for Delta2 expression (red) and electroporated with FGF8/17/18>GFP reporter construct (green nuclei) and FGF8/17/18>Eph3ΔC overexpression construct.
Delta2 is expressed in all the A9.30 progeny (ie. A11.120, A11.119, A11.118 and A11.117).
Original comment: Ectopic Delta2 expression is seen in A11.119 upon loss of A10.59 fate (n56/71) and is interpreted as loss of Notch activation in A11.119 according to the model of lateral inhibition.
The A9.30 lineage is visualized using the FGF8/17/18 reporter construct (green nuclei, diagram).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of Ghost database entry from Imai et al. (2004) Development 131(16)

Original annotation:
Gene name: Delta-like.
Expression pattern: nervous system, Nerve cord, epidermis.
Comment : epidermis aroud the palp and in the tip of the tail.

Aniseed annotation :
A staining has also been detected in the nervous system.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) epidermis - tail epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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A tailbud wild type embryo probed for Delta-like expression. The first picture is a lateral view and the second a dorsal view. Expression was detected in central nervous system and anterior ventral head epidermis (a7.11 line). Aniseed Comment: update of annotation of (Imai et al., 2004), based on (Imai et al., 2006).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior ventral head epidermis (a7.11 line) - central nervous system -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior sensory vesicle - apical trunk epidermal neurone precursors (ATEN) - caudal epidermal neurone (CEN) precursors - palp neurone precursors - rostral trunk epidermal neurone precursors (RTEN) (a8.26 line) - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Mid tailbud stage wild type embryo probed for Delta-like expression. No picture available in the article.

Original Annotation :
Delta-like expression in the visceral ganglion is localized in A12.238, A11.118 and A11.117 cell pairs (visceral ganglion).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Original annotation: expression in ESNs (epidermal sensory neurons). LacZ MO as a control for Tox and Ascl.b MO experiment. Lateral view of the embryo is shown.
Aniseed annotation: weak expression in the visceral ganglion.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Original annotation: expression lost compared to the control.
Aniseed annotation: weak expression in the visceral ganglion.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Original annotation : expression down regulated in embryos injected with Ascl.b MO.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Original annotation : expression down regulated in embryos injected with Tox MO. Lateral view of the embryo is shown.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) dorsal anterior sensory vesicle - neurohypophysis primordium - tail nerve cord - ventral posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 22 (mid tailbud II)

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Lateral view of a mid tailbud stage embryo stained with Delta 2. White lines demarcate boundaries between different focal planes. Expression was observed in the epidermal sensory neurones.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior sensory vesicle - caudal epidermal neurone (CEN) precursors - posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral view of a late tailbud stage embryo stained with Delta2. Expression was observed in the epidermal sensory neurones.

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) anterior sensory vesicle - caudal epidermal neurones (CEN) - posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral and high magnification views of a late tailbud embryo, electroporated with a lacZ transgene containing the 5' flanking Ci-multidom region (#1 construct in the publication). The staining pattern recapitulates the expression observed in ANB by in situ hybridization (arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neurohypophysis primordium -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral and high magnification views of a late tailbud embryo, electroporated with a lacZ transgene containing the 5' flanking Ci-multidom region (#3 construct in the publication). An ectopic staining in the notochord (black arrowheads) was detected in a fraction of the embryos, in addition to the staining in the ANB (arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neurohypophysis primordium - notochord -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral and high magnification views of a late tailbud embryo, electroporated with a lacZ transgene containing the 5' flanking Ci-multidom region (#2 construct in the publication). An ectopic staining in a small region of the sensory vesicle (white arrowhead) was detected in a fraction of the embryos, in addition to the staining in the ANB (arrow).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) neurohypophysis primordium -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Views of larvae showing reporter expression of the region upstream of delta2 in a part of trunk, epidermis around ESN and tail epidermis(dorsal midline and beta-region).

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) head epidermis - tail epidermis -

C. robusta formely Ciona int. type A, Stage 48 (young adult)

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Conversion to Aniseed format of Ghost database entry from Ogasawara et al., Dev Genes Evol. 2002; 212:173-185. Original annotation: . Expression pattern: not detected

Stained molecule KH2012:KH.L50.6 (DLK2; DLL1)
Stained region(s) No expression -