27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
0 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
1 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
3 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
2 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
5 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
3 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
0 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
6 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
3 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
1 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
0 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
1 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
1 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
2 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
4 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
5 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
2 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
1 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
11 clone(s) / 188,431 |
3 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).
| Assay platform | Ion Torrent PGM | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 20-365 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
| Mutant | ||
| Deregulated molecule(s) | KH2012:KH.C8.890 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
51 results
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : Pem-like localized signal was detected.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | whole embryo - |
Fertilized eggs just after the second phase of ooplasmic segregation viewed from lateral side. Localization or distribution pattern of Ci-Wnt5 maternal transcript : Pem localized signal was detected.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | whole embryo - |
Localization or distribution pattern of Ci-Wnt5 (which is a maternal transcript) in 8-cell stage embryos, revealed by whole-mount in situ hybridization. Embryos viewed from the right side and from the posterior side. Pem localized signal was detected in B4.1 cell pair.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B4.1 cell pair - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : No distinct zygotic signal was detected.
Comment : The expression in the posterior end/endodermal strand is maternal.
Aniseed annotation :
Staining is detected in B5.2.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B5.2 cell pair - |
Animal and vegetal views of a late 32-cell stage embryo. The zygotic expression of Ci-Wnt5 was detected in B6.1, B6.2 and B6.4 blastomeres. Arrow indicate localized maternal transcripts.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.3 cell pair - B6.4 cell pair - |
Animal and vegetal views of a late 32-cell stage embryo. Effects of overexpression of Ci-macho1 revealed by whole-mount in situ hybridization. Ci-Wnt5 expression was activated in all blastomeres of Ci-macho1-overexpressing embryos. Arrow indicates localized maternal transcripts.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | whole embryo - |
Vegetal and animal views of a late 32-cell stage embryo. Effects of functional suppression of Ci-macho1 revealed by whole-mount in situ hybridization. Ci-Wnt5 expression was inactivated in Ci-macho1-suppressed embryos. Arrow indicates localized maternal transcripts.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : B6.1, B6.2, B6.3.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - B6.3 cell pair - B6.4 cell pair - |
Vegetal view of a WT Ciona robusta late 32-cell stage embryo probed for Wnt5 expression.
Expression is observed in B6.1 and B6.2 blastomere pairs.
In schematic representation of gene expression, notochord and nerve cord precursors mother cells are in purple. Blastomeres with gene expression are marked by dots.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B6.1 cell pair - B6.2 cell pair - |
Vegetal view of a 64-cell stage wild type embryo probed for Wnt5 expression.
Original annotation :
Gene name :Wnt5.
Expression pattern : A7.4, A7.8, B7.3, B7.4, B7.7, B7.8.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | A7.4 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a 64-cell stage embryo probed for Wnt5 expression following egg micro-injection of a FoxD-a/b MO. Wnt5 was down-regulated in A-line nerve cord cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B7.3 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : A7.4, A7.8, B7.3, B7.4, B7.7, B7.8.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | A7.4 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal view of a WT Ciona robusta 64-cell stage embryo probed for Wnt5 expression.
Expression is observed in A7.4, A7.8, B7.3, B7.4, B7.7, B7.8 and B7.6 blastomere pairs.
In schematic representation of gene expression, notochord precursors are shown in red and nerve cord precursors in blue. Blastomeres with gene expression are marked by dots.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | A7.4 cell pair - A7.8 cell pair - B7.3 cell pair - B7.4 cell pair - B7.6 cell pair - B7.7 cell pair - B7.8 cell pair - |
Vegetal side of a 110-cell stage embryo stained for Ci-Wnt5 mRNA by in situ hybridization.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B7.6 cell pair - B8.15 cell pair - B8.16 cell pair - B8.5 cell pair - B8.7 cell pair - B8.8 cell pair - |
Aniseed Comment : update of annotation of (Imai et al,.2004), based on (Imai et al,.2006).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - B8.5 cell pair - B8.7 cell pair - B8.8 cell pair - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : B8.5, B8.7, B8.8, B7.6, B7.7, B8.15, B8.16.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - B8.5 cell pair - B8.7 cell pair - B8.8 cell pair - |
Vegetal view of a WT Ciona robusta 112-cell stage embryo probed for Wnt5 expression.
Expression is observed in B8.8, B8.7, B8.5, B7.7, B8.15, B8.16 and B7.6 blastomere pairs.
In schematic representation of gene expression, notochord precursors are shown in red and nerve cord precursors in blue. Blastomeres with gene expression are marked by dots.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | B7.6 cell pair - B7.7 cell pair - B8.15 cell pair - B8.16 cell pair - B8.5 cell pair - B8.7 cell pair - B8.8 cell pair - |
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | No expression - |
Vegetal view with anterior to the top and posterior view of a mid-gastrula stage embryo probed for Ci-wnt5 expression. DAPI staining in blue.
Original annotation: Expression was observed in tailtip epidermal cells (indicated by green arrowheads).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior tail epidermis - tail muscles - |
Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline anterior tail epidermis - epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - tail muscles - ventral anterior tail epidermis - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : muscle, epidermis.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | epidermis - tail muscles - |
Lateral view of a mid neurula wild type embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - tail epidermis - |
Lateral view of a mid neurula embryo probed for Ci-Wnt5 expression following the injection of Ci-Hox12-MO.
Original annotation:
The expression of Ci-Wnt5 in Hox12MO-injected embryos at neurula and early tailbud stage was investigated. Expression of Ci-Wnt5 was detected in the tail tip epidermis in 100% of the Hox12MO-injected embryos at both stages.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - tail epidermis - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : muscle, epidermis.
Comment : The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | epidermis - tail muscles - |
Lateral view with anterior to the top and posteror view of an early tailbud stage embryo probed for Ci-wnt5 expression. DAPI staining in blue.
Original annotation: Expression was observed in tailtip epidermal cells (indicated by green arrowheads).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo probed for Wnt5 expression.
Original annotation: Expression was observed in the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorso lateral posterior tail epidermis - lateral posterior tail epidermis - posterior endodermal strand (B8.11 line) - ventral lateral posterior tail epidermis - ventral midline posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with Wnt5 after retinoic acide treatment (1.5μM) at the late gastrula stage.
Original annotation: Expression was lost in the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - ventral lateral posterior tail epidermis - ventral midline posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with Wnt5 after DEAB treatment (150μM) at the late gastrula stage.
Original annotation: Expression was observed in in the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with Wnt5 after bFGF treatment (100ng/ml) at the late gastrula stage.
Original annotation: Expression was expanded anteriorly from the the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior tail epidermis - |
Lateral view with anterior to the left of a mid-tailbud stage embryo probed for Wnt5 expression after injection of a dominant-negative forme of the Ciona FGF receptor (Ci-FGFR) at the late gastrula stage.
Original annotation: Expression was lost or decreased in the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - |
Lateral view with anterior to the left of a mid-tailbud stage embryo stained with Wnt5 after U0126 treatment (10μM) at the late gastrula stage.
Original annotation: Expression was lost in the cells around the tailtip.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | endodermal strand - lateral posterior tail epidermis - ventral lateral posterior tail epidermis - ventral midline posterior tail epidermis - |
Lateral view of an early tailbud wild type embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of an early tailbud embryo probed for Ci-Wnt5 expression following the injection of Ci-Hox12-MO.
Original annotation:
The expression of Ci-Wnt5 in Hox12MO-injected embryos at neurula and early tailbud stage was investigated. Expression of Ci-Wnt5 was detected in the tail tip epidermis in 100% of the Hox12MO-injected embryos at both stages.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Aniseed comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : epidermis.
Comment : epidermis near the tip of the tail; The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | epidermis - |
Lateral view of a mid tailbud DMSO-treated embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage. We identified Ci-Fgf8/17/18 and Ci-Wnt5 as the genes expressed in the tail tip epidermis in normal mid-tailbud embryos.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression. Embryos were treated with U0126 (a MEK inhibitor).
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was observed on the dorsal side close to the tail tip of embryos treated with U0126.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline posterior tail epidermis - posterior endodermal strand (B8.11 line) - |
Lateral view of a mid tailbud DMSO-treated embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage. We identified Ci-Fgf8/17/18 and Ci-Wnt5 as the genes expressed in the tail tip epidermis in normal mid-tailbud embryos.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression and treated with U0126.
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was observed on the dorsal side close to the tail tip of embryos treated with U0126.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior endodermal strand (B8.11 line) - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression. Embryos were treated with SU5402 ( FGF receptor inhibitor).
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was observed at the tail end of embryos treated with SU5402.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud wild type embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage. We identified Ci-Fgf8/17/18 and Ci-Wnt5 as the genes expressed in the tail tip epidermis in normal mid-tailbud embryos.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression following the injection of Ci-Hox12-MO.
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was extensively reduced in Hox12MO-injected embryos, whereas expression outside of the tail tip was unaffected.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression following the co-injection of Ci-Hox12-MO and the mRNA of Ci-Hox12.
Original annotation:
The expression of Ci-Wnt5 at the tail tip was recovered.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud DMSO-treated embryo probed for Ci-Wnt5 expression.
Original annotation:
The expression of Ci-Wnt5 in the posterior epidermis becomes detectable by WISH at the gastrula stage. We identified Ci-Fgf8/17/18 and Ci-Wnt5 as the genes expressed in the tail tip epidermis in normal mid-tailbud embryos.
Aniseed annotation:
Posterior blastomeres endodermal B7.6 cells produce two daughter cells names B8.11 and B8.12 cells (Shirae-Kurabayashi et al., 2006). Ci-Wnt5 seems to be expressed in B8.11 cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression and treated with U0126, a MEK inhibitor.
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was observed on the dorsal side close to the tail tip of embryos treated with U0126.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline posterior tail epidermis - posterior endodermal strand (B8.11 line) - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression. Embryos were treated with SU5402 ( FGF receptor inhibitor).
Original annotation:
Expression of Ci-Wnt5 in the tail tip epidermis was observed at the tail end of embryos treated with SU5402.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Lateral view of a mid tailbud embryo probed for Ci-Wnt5 expression following the injection of Ci-Fgf8/17/18-MO.
Original annotation: Expression of Ci-Wnt5 was also detected in the tapered tail end of the embryos injected with the Fgf8/17/18MO.
Aniseed Annotation :
It seems that the expression in the head epidermis and in the endodermal strand was lost.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | posterior tail epidermis - |
Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :Wnt5.
Expression pattern : epidermis.
Comment : epidermis near the tip of the tail and around the neck region; The expression in the posterior end/endodermal strand is maternal.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | epidermis - |
Lateral view with anterior to the top of a mid-tailbud stage embryo probed for Ci-wnt5 expression.
Original annotation: Expression was observed in tailtip epidermal cells.
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | head epidermis - posterior endodermal strand (B8.11 line) - posterior tail epidermis - |
Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).
| Stained molecule | KH2012:KH.L152.45 (WNT4; WNT5A; WNT5B) |
| Stained region(s) | dorsal midline anterior tail epidermis - dorsal midline posterior tail epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - ventral midline anterior tail epidermis - ventral midline posterior tail epidermis - |
