Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No distinct zygotic signal was detected.

Aniseed annotation: no picture available for this stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : No distinct zygotic signal was detected

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : epidermis

Dorsal view of an early neurula stage embryo, showing expression of ci-FoxH-a. Expression of ci-FoxH-a was detected by whole-mount in situ hybridization.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : nervous system, epidermis.
Comment : the expression in the neural cells are especially strong.

Dorsal and lateral views of a mid neurula stage embryo, showing expression of ci-FoxH-a. Expression of ci-FoxH-a was detected by whole-mount in situ hybridization.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the entire body was weakly stained. Expression pattern: Papilla

Lateral and dorsal view of initial tailbud wild type embryos probed for Ci-FoxHa expression.

Original annotation: Ci-FoxHa expression covered two lateral bands linked anteriorly by a transverse ventral stripe, leaving a posterior unlabelled band at the ventral midline of the sensory vesicle. Expression also extend to the rostral epidermis.

Aniseed annotation: according to the picture, it seems that expression is detected in the central nervous system (except in the tail nerve cord), the palps and in the tail epidermis.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the entire body was weakly stained. Expression pattern: Papilla Mesenchyme

Dorsal view a mid tailbud stage wild type embryo probed for Fox H-a expression.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : nervous system, epidermis
Comment : nervous system: brain and papilla neurons; epidermis: mainly tail epidermis.

Views of an early tailbud stage embryo, showing expression of ci-FoxH-a. Expression of ci-FoxH-a was detected by whole-mount in situ hybridization.

Dorsal and lateral view of early tailbud stage wild type embryos probed for Ci-FoxHa expression.

Original annotation: expression was restricted to the rostral epidermis and sensory vesicle.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the entire body was weakly stained. Expression pattern: Notochord Papilla

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxH-a.
Expression pattern : nervous system, unidentifiable
Comment : nervous system: brain and papilla neurons; epidermis: mainly tail epidermis

Lateral and dorsal views of a mid tailbud stage embryo, showing expression of ci-FoxH-a. Expression of ci-FoxH-a was detected by whole-mount in situ hybridization.

Lateral view of an early tailbud stage embryo. Expression of ci-FAST11 is revealed by whole-mount in situ hybridization. Expression of Ci-Fast11 is observed in sensory vesicles and palps.

Lateral view of mid tailbud wild type embryos probed for Ci-FoxHa expression.

Original Annotation : Expression was detected in the anterior sensory vesicle, rostral epidermis.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: the entire body was weakly stained. Expression pattern: Endoderm

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Dorsal and lateral views of a late tailbud stage embryo, showing expression of ci-Fast11. Expression of ci-Fast11 was detected by whole-mount in situ hybridization.