27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
0 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
0 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
0 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
0 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
7 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
1 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
0 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
0 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
1 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
0 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
2 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
0 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
0 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
3 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
3 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
1 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
1 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
0 clone(s) / 188,431 |
2 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
14 results
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | whole embryo - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | tail nerve cord - visceral ganglion - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - visceral ganglion - |
Lateral view of a WT Ciona robusta late tailbud probed for Cplx2 expression.
Cplx2 is expressed in the sensory vesicle and in the visceral ganglion.
"n" is the number of scored embryos ; % corresponds to the proportion of embryos showing similar staining patterns.
Black arrow indicates the territory of the visceral ganglion where no alteration of gene expression can be observed because outside the domain of expression of pGsx.
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - posterior sensory vesicle - visceral ganglion - |
Lateral view of a Ciona robusta late tailbud stage embryo probed for Cplx2 expression and electroporated with pGsx>OC::VP16.
Cplx2 is strongly expressed in the sensory vesicle while its expression in the visceral ganglion remained unaltered.
In 65% of the embryos in which constitutively active Onecut was overexpressed in the sensory vesicle, Cplx2 was overexpressed in the progenitor cells of the sensory vesicle.
"n" is the number of scored embryos ; % corresponds to the proportion of embryos showing similar staining patterns.
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - posterior sensory vesicle - visceral ganglion - |
Lateral view of a Ciona robusta late tailbud stage embryo probed for Cplx2 expression and electroporated with pGsx>OC::WRPW.
Cplx2 expression is lost (65%) or down-regulated (15%) in the sensory vesicle, while its expression in the visceral ganglion remained unaltered.
"n" is the number of scored embryos ; % corresponds to the proportion of embryos showing similar staining patterns.
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | visceral ganglion - |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) |
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - neck - posterior sensory vesicle - visceral ganglion - |
Conversion to aniseed format of DBTGR database entry from Kusakabe team.
Original annotation:
Papilla; brain vesicle; visceral ganglion; notochord.
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - notochord - posterior sensory vesicle - visceral ganglion - |
Conversion to Aniseed format of Ghost database entry from Kusakabe T et al., Dev Biol. 2002; 242:188-203. Original annotation: . Expression pattern: Brain Ganglion Nervecord
| Stained molecule | KH2012:KH.C8.717 (CPLX1; CPLX2) |
| Stained region(s) | anterior sensory vesicle - neck - posterior sensory vesicle - tail nerve cord - visceral ganglion - |
