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GENE CARD

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EST count

27 results

Library Name Percentage Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal 0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library 1 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult 1 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo 0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg 0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva 7 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo 8 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library 0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library 0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis 0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library 0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva 0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells 0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle 0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo 0 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula 0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad 0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex 0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart 4 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland 0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal 1 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal 1 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal 3 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal 0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile 0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) 0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) 0 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

2 results

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

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Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34139

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The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform Illumina HiSeq 2500
Layout Single-end
Stranded Yes
Read length 101
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 1
Deregulated molecule(s) KH2012:KH.C4.125 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 2
Deregulated molecule(s) KH2012:KH.C2.573 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

33 results

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : endoderm, epidermis
Comment : the ventral part of the trunk epidermis

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior head endoderm - ventral head epidermis -

C. robusta formely Ciona int. type A, Stage 16 (late neurula)

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Ventral view of a late neurula stage embryo probed for Nkx (green) expression. Nuclei of TVCs and ATMs are marked by β-gal antibody staining (red). Inset panels display magnified views of the B7.5 lineage. Note that the TVCs are the more anterior lineage, as marked by arrowheads in the first column.

Original annotation:
Nkx was expressed in an overlapping region of the anterior/ventral epidermis and in portions of the ventral endoderm.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Notochord Epidermis Nerve cord Mesenchyme

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) epidermis - notochord - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis Nerve cord Mesenchyme

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Lateral view of an early tailbud stage embryo probed for Nkx (green) expression. Nuclei of TVCs and ATMs are marked by β-gal antibody staining (red). Inset panels display magnified views of the B7.5 lineage. Note that the TVCs are the more anterior lineage, as marked by arrowheads in the first column.

Original annotation:
The expression patterns of Nkx remained unaltered. the TVCs did not appear to express either Nkx during these stages.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : endoderm, mesenchyme, epidermis
Comment : the ventral part of the trunk epidermis; TVC mesenchyme; trunk endoderm

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior head endoderm - trunk ventral cells (B7.5 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Early-tailbud embryos electroporated with Mesp > lacZ, detected by immunofluorescence (red), and hybridized with NK4 antisense RNA probes (green).

Original annotation:
They observed conspicuous TVC specific expression of GATAa and Tolloid, while NK4 and Bmp2/4 expression could not be detected above background levels.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) epidermis - head endoderm -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Ventral view of a WT Ciona robusta early tailbud embryo probed for Nk4 (KH.C8.482) expression.
Expression is observed in the ventral trunk epidermis.
Scale bar : 100 µm

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) ventral head epidermis -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Ventral view of a Ciona robusta early tailbud embryo probed for Nk4 (KH.C8.482) expression and injected with morpholinos against Admp.
No expression can be observed.
Original annotation : NK4 expression, which marks the ventral epidermis in the trunk, was suppressed in Admp morphants.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Ventral view of a Ciona robusta early tailbud embryo probed for Nk4 (KH.C8.482) expression and injected with morpholinos against Bmp2/4.
Weak expression is observed in the ventral trunk epidermis.
Original annotation : In Bmp2/4 morphants, Nk4 expression was reduced.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) ventral head epidermis -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Ventral view of a Ciona robusta early tailbud embryo probed for Nk4 (KH.C8.482) expression and injected with morpholinos against Pinhead (KH.C2.1069).
Expression is observed in ventral and lateral head epidermis.
Original annotation : In Pinhead morphants, NK4 expression was expanded to the lateral regions (of trunk epidermis).

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) dorso lateral head epidermis - ventral head epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation:
Expression pattern: Epidermis Endoderm Brain Mesenchyme Trunk lateral cells Trunk ventral cells.

Aniseed annotation : a staining has been detected in the nervous system but also in the visceral ganglion.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) endoderm - epidermis - nervous system - trunk lateral cells (A7.6 line) - trunk ventral cells (B7.5 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Picture 1: Side view (anterior is to the left) of a mid-tailbud embryo stained for Ci-Nkx mRNA by in situ hybridization. Note staining of the TVCs (arrow)

Picture 2: Ventral view (anterior is to the left) of a mid-tailbud embryo stained for Ci-Nkx mRNA by in situ hybridization. Note staining of the TVCs (arrow)

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) posterior ventral head endoderm - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) - ventral head epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed for Nkx (green) expression. Nuclei of TVCs and ATMs are marked by β-gal antibody staining (red). Inset panels display magnified views of the B7.5 lineage. Note that the TVCs are the more anterior lineage, as marked by arrowheads in the first column.

Original annotation:
Nkx expression in the TVCs rose to detectable levels.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo co-electroporated with Mesp-lacZ (red) and Hl-GATAa-DBD and probed for Nkx (green) expression. The construct Hl-GATAa-DBD was included in embryos for a more rigourous control : this construct contains the GATAa DNA binding domain alone which is expressed in the TVCs.

Original annotation:
In Hl-GATAa- DBD control embryos, Nkx was consistently expressed in the trunk ventral cells.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo co-electroporated with Mesp-lacZ (red) and Hl-GATAa-WRPW and probed for Nkx (green) expression. Construct Hl-GATAa-WRPW was used to disrup GATAa activity.

Original annotation:
In Hl-GATAa-WRPW embryos, TVC expression of Nkx was eliminated or significantly reduced while expression in adjacent lineages was not affected.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo co-electroporated with Mesp-lacZ (red) and Ttf-GATAa-DBD and probed for Nkx (green) expression. The construct Ttf-GATAa-DBD was used as a control.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo co-electroporated with Mesp-lacZ (red) and Ttf-GATAa-WRPW and probed for Nkx (green) expression. The construct Ttf-GATAa-DBD was used as a control.

Original annotation:
There was no detectable difference in the TVC expression of Nkx between Ttf-GATAa-WRPW and control embryos.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Sagital view of WT mid tailbud embryo showing expression of NK4 (Nkx2.5) by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation. NK4 is expresed throughout the ventral head endoderm and in the TVCs.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) posterior ventral head endoderm - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Control: sagital view of WT mid tailbud embryo showing expression of NK4 (Nkx2.5) by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation.

Experimental embryos: sagital view of mid tailbud embryos electroporated with mesp:FoxF-WRPW showing expression of NK4 by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation. Note that in some embryos (D), NK4 expression is not affected in heart progenitors, while in others it is blocked (F).

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) posterior ventral head endoderm - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Control: sagital view of WT mid tailbud embryo showing expression of NK4 by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation.

Experimental embryos: sagital view of mid tailbud embryos overexpressing dnFGFR and showing expression of NK4 by fluorescent ISH (green), and the position of the B7.5 lineage (red, tail staining probably artefactual) marked by immunohistochemistry following mesp::NLS-LacZ electroporation. NK4 expression is blocked in TVC heart progenitors, which also do not migrate.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) posterior ventral head endoderm - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Control: sagital view of WT mid tailbud embryo showing expression of NK4 (Nkx2.5) by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation.

Experimental embryos: sagital view of mid tailbud embryos electroporated with mesp::ETS1/2 showing expression of NK4 (Nkx2.5) by fluorescent ISH (green), and the position of the B7.5 lineage (red) marked by immunohistochemistry following mesp::NLS-LacZ electroporation. NK4 (Nkx2.5) expression is strong in all B7.5 progeny, which all adopt a TVC fate.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral primary muscle lineage (B7.5 line) - posterior ventral head endoderm - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK4.
Expression pattern : endoderm, mesenchyme, epidermis.
Comment : the ventral part of the trunk epidermis; TVC mesenchyme; trunk endoderm

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Notochord Epidermis

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) epidermis - notochord -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral view of a late tailbud stage embryo probed for Nkx (green) expression. Nuclei of TVCs and ATMs are marked by β-gal antibody staining (red). Inset panels display magnified views of the B7.5 lineage. Note that the TVCs are the more anterior lineage, as marked by arrowheads in the first column.

Original annotation:
Nkx expression in the TVCs appeared to be greatly reduced.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior ventral head epidermis (a7.11 line) - posterior ventral head endoderm (B7.2 line) - trunk ventral cells (B7.5 line) - ventral anterior head endoderm (A7.1 line) -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Picture 1: Side view (anterior is to the left) of a tadpole embryo stained for Ci-Nkx mRNA by in situ hybridization.

Picture 2: Ventral view (anterior is to the left) of a tadpole embryo stained for Ci-Nkx mRNA by in situ hybridization.

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s) anterior head endoderm - posterior ventral head endoderm -

C. robusta formely Ciona int. type A, Stage 48 (young adult)

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Side view (anterior is to the left) of a young adult (36h after metamorphosis) embryo stained for Ci-Nkx mRNA by in situ hybridization. Note staining of the differentiating heart (arrowhead).

Stained molecule KH2012:KH.C8.482 (NKX2-3; NKX2-5; NKX2-6)
Stained region(s)