27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
0 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
1 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
1 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
4 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
17 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
13 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
2 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
2 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
6 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
0 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
1 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
3 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
0 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
0 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
2 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
0 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
1 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
2 clone(s) / 188,431 |
3 results
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).
| Assay platform | Ion Torrent PGM | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 20-365 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
| Mutant | ||
| Deregulated molecule(s) | KH2012:KH.C8.890 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 6a (early 32-cell) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 10 (112-cell) | ||
The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.
| Assay platform | Illumina HiSeq 2500 | |
| Layout | Single-end | |
| Stranded | Yes | |
| Read length | 101 | |
| Normalized data types |
|
|
| Wild type | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 1 | ||
| Deregulated molecule(s) | KH2012:KH.C4.125 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
| Mutant 2 | ||
| Deregulated molecule(s) | KH2012:KH.C2.573 - | |
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 13 (late gastrula) | |
22 results
64-cell stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
64-cell stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
110-cell stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
110-cell stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
Mid gastrula stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
Mid gastrula stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) |
Vegetal view of a gastrula stage embryo showing Prickle expression. Expression was examined by whole-mount in-situ hybridization in embryos that carried Ci-FoxD/lacZ.
Original annotation:
Expression of prickle mRNA was detected in the lateral neural plate cells at the late gastrula stage.
Aniseed annotation:
According to the picture, it seems that cells stained are b-line neural plate, A.30, A9.32 and A9.29 cell pairs.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | A9.29 cell pair - A9.30 cell pair - A9.32 cell pair - b line neural plate - |
Vegetal view of a gastrula stage embryo showing Prickle expression. Expression was examined by whole-mount in-situ hybridization in embryos that carried Ci-FoxD/Ci-Nodal. Red arrowheads indicate ectopic expression.
Original annotation:
Overexpression of Ci-nodal induced ectopic activation of prickle in the medial neural plate cells and anterior epidermis.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | b line neural plate - head epidermis - row II neural plate - row I neural plate - |
Vegetal view of a gastrula stage embryo showing Prickle expression. Expression was examined by whole-mount in-situ hybridization in SB-treated embryos.
Original annotation:
SB431542 completely suppressed the expression of prickle in the gastrula.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | No expression - |
Neural plate view of a late gastrula stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
Aniseed annotation:
According to the picture, it seems that there is weak expression in the tail muscle.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - tail muscles - |
Neural plate view of a late gastrula stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
Original annotation:
Staining is also observed in the tail muscle.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - tail muscles - |
Neural plate view of a Mid neurula stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - |
Neural plate view of a Mid neurula stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | A10.30 cell pair - A10.58 cell pair - A10.63 cell pair - A10.64 cell pair - b10.73 cell pair - b10.74 cell pair - notochord - row I-A neural plate - |
Dorsal view of a initial tailbud stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - |
Dorsal view of a initial tailbud stage wild type probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - tail nerve cord - |
Lateral view of a Mid tailbud stage wild type embryo probed for Ci-pk2 expression. Expression was detected from the late gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | notochord - |
Mid tailbud stage embryo probed for Ci-pk2 expression following injection of of Ci-fkh/Ci-Bra.
Original annotation:
The Ciona pk2 gene is expressed ectopically in the mutant as compared with the control embryo. In particular, staining is observed both in the endogenous notochord and transformed endoderm (no picture available in the paper).
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | endoderm - notochord - |
Tailbud stage embryos probed for Ci-pk expression. No picture available in the paper.
Original Annotation :
Expression in notochord and epidermis.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | epidermis - notochord - |
Lateral view of a Mid tailbud stage wild type embryo probed for Ci-pk1 expression. Expression was detected from the gastrula stage.
| Stained molecule | KH2012:KH.C8.316 (PRICKLE1; PRICKLE2; PRICKLE3) |
| Stained region(s) | anterior sensory vesicle - neck - notochord - posterior sensory vesicle - tail nerve cord - visceral ganglion - |
