27 results
| Library Name | Percentage | Expression |
|---|---|---|
| Ciona intestinalis whole animal Ciona intestinalis whole animal |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library |
|
0 clone(s) / 5,577 |
| Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult |
|
0 clone(s) / 31,366 |
| Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo |
|
0 clone(s) / 15,579 |
| Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg |
|
0 clone(s) / 31,100 |
| Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva |
|
1 clone(s) / 26,704 |
| Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo |
|
0 clone(s) / 26,680 |
| directional larval cDNA library directional larval cDNA library |
|
0 clone(s) / 39 |
| Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library |
|
0 clone(s) / 0 |
| K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis |
|
0 clone(s) / 5,468 |
| Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library |
|
0 clone(s) / 0 |
| Ciona intestinalis larva Ciona intestinalis larva |
|
0 clone(s) / 0 |
| Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells |
|
0 clone(s) / 29,579 |
| Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle |
|
0 clone(s) / 2,556 |
| Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo |
|
0 clone(s) / 16,939 |
| Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula |
|
0 clone(s) / 25,258 |
| Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad |
|
0 clone(s) / 16,936 |
| Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex |
|
1 clone(s) / 10,463 |
| Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart |
|
0 clone(s) / 13,243 |
| Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland |
|
0 clone(s) / 17,765 |
| Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal |
|
0 clone(s) / 17,872 |
| Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal |
|
0 clone(s) / 107,314 |
| Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal |
|
0 clone(s) / 24,372 |
| Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal |
|
0 clone(s) / 17,126 |
| Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile |
|
0 clone(s) / 3,798 |
| Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) |
|
0 clone(s) / 2,031 |
| Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) |
|
0 clone(s) / 188,431 |
1 result
OVERVIEW
Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.
Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.
OVERVIEW
Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.
Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.
| Assay platform | Illumina HiSeq 2000 | |
| Layout | Paired-end | |
| Stranded | Yes | |
| Read length | 49 | |
| Normalized data types |
|
|
| Wild type - Replica 1 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
| Wild type - Replica 2 | ||
| Territories | whole embryo - | |
| Biomaterial(s) | C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg) | |
| C. robusta formely Ciona int. type A, Stage 8 (64-cell) | ||
| C. robusta formely Ciona int. type A, Stage 11 (early gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 12 (mid gastrula) | ||
| C. robusta formely Ciona int. type A, Stage 15 (mid neurula) | ||
| C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I) | ||
| C. robusta formely Ciona int. type A, Stage 26 (hatching larva) | ||
65 results
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | whole embryo - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | whole embryo - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : No distinct zygotic signal was detected.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : nervous system.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | A10.31 cell pair - |
Dorsal views of early tailbud stage embryo expressing En. The upper panel shows WISH signals, the middle DAPI signals and the lower the merged images.
Arrowheads indicate nuclei of the cells that express En. Open arrows indicate the constrictions in the row of nuclei of the lateral cells. Red dots indicate nuclei of the landmark cells.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal precursors - |
Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : nervous system, nerve cord.
Comment : two pairs of cells in the brain and in the nerve cord.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the early tailbud stage. En expression is shown in green, Pax2/5/8a in red.
Original annotation:
Ci-En is expressed in the visceral ganglion and in the posterior sensory vesicle. Ci-Pax2/5/8 is expressed between the two Ci-En expression domains.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the early tailbud stage. En expression is shown in green, Otx in red.
Original annotation:
Ci-En is expressed in the visceral ganglion and in posterior sensory vesicle. Ci-En is expressed in two distinct domains of the CNS and the posterior boundaries of the first Ci-En domain and the Ci-Otx domain coincide exactly.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the early tailbud stage. En expression is shown in green, Hox1 in red.
Original annotation:
Ci-En is expressed in the visceral ganglion and in the posterior sensory vesicle. The second domain of Ci-En overlaps with the posterior part of the first domain of Hox1, while the first domain of Ci-En is just ahead of the first domain of Ci-Hox1.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the early tailbud stage. En expression is shown in green.
Original annotation:
Ci-En is expressed in the visceral ganglion and in posterior sensory vesicle. The first domain of Ci-SoxB1 overlaps with the posterior part of the second domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Dorsal view of an early tailbud stage wild type embryo probed for en expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
En expression was detected in A11.63 and A11.64 cell pairs (posterior sensory vesicle) and in A11.119 and A11.120 cell pairs (anterior visceral ganglion).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Dorsal view of an early tailbud stage embryo probed for en expression following the injection of Cdx-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
Ectopic was observed in the nerve cord (A11.116 and A11.115 cell pairs) (black arrow).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - tail nerve cord - visceral ganglion - |
Dorsal view of an early tailbud stage embryo probed for en expression following the injection of FGF8/17/18-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
Ectopic expression was observed in the neck (A11.62 and A11.61 cell pairs) (black arrow).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Dorsal view of an early tailbud embryo probed for en expression following the injection of neurogenin-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
Ectopic expression was observed in the nerve cord (A11.115 and A11.116 cell pairs) (black arrow).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - tail nerve cord - visceral ganglion - |
Dorsal view of an early tailbud stage embryo probed for en expression following the injection of Pax6-MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.
Original Annotation :
Expression was lost following the injection of Pax6-MO.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Dorsal view of at early tailbud stage wild type embryo probed for Ci-En expression.
The insert shows in red cells from the posterior sensory vesicle (PSV) and light-blue cells from the middle part of the visceral ganglion (VG). Broken white lines indicate the boundaries of the PSV/neck and the neck/VG.
Original Annotation :
Ci-En expression was detected in A11.64 and A11.63 cell pairs (posterior sensory vesicle) and in A11.120 and A11.119 cell pairs (anterior visceral ganglion).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Dorsal view of an early tailbud embryo probed for Ci-En expression, following the injection of Fgf8/17/18 MO.
The insert shows in red cells from the posterior sensory vesicle (PSV) and light-blue cells from the middle part of the visceral ganglion (VG). Broken white lines indicate the boundaries of the PSV/neck and the neck/VG.
Original Annotation :
Ectopic expression was detected in A11.62 and in A11.61 cell pairs (neck).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Lateral view of the motor ganglion of a WT Ciona robusta early tailbud embryo probed for Engrailed expression (red) and electroporated with Fgf8/17/18>LacZ reporter construct (green nuclei).
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors (arrowheads).
Original comment: Nuclei of the A9.30 lineage are visualized by immunofluorescence detection of nuclear β-galactosidase reporter (green) driven by the FGF8/17/18 driver (cf diagram).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Lateral view of an early tailbud stage embryo probed for En and Fgf9/16/20 expression.
Original annotation
Three others Fgfs were also found to be expressed in the CNS.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Lateral view of an early tailbud stage embryo probed for En and Pax/2/5/8 expression.
Original annotation:
The posterior expression domain is just behind the neck region marked by Ci-Pax2/5/8.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Lateral view of an early tailbud stage embryo probed for En and Fgf8/17/18.
Initial annotation
Ci-Fgf8/17/18 is expressed in the same region as the posterior Ci-En expression domain.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Lateral view of an early tailbud stage embryo probed for En and Fgf3/7/10/22 expression
Original annotation :
Ci-Fgf3/7/10/22 is expressed in the ventral row of the nerve cord and the posterior part of the brain.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Lateral and dorsal views of an early tailbud stage embryo probed for En expression.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Lateral view of an early tailbud stage embryo probed for Otx and En expression.
Original annotation:
The anterior expression domain of Ci-En corresponds to the most posterior region of the grain that is marked by Ci-Otx.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | posterior sensory vesicle - visceral ganglion - |
Dorsal views of mid tailbud stage embryo expressing En. The upper panel shows WISH signals, the middle DAPI signals and the lower the merged images.
Arrowheads indicate nuclei of the cells that express En. Open arrows indicate the constrictions in the row of nuclei of the lateral cells. Red dots indicate nuclei of the landmark cells.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal precursors - neck - |
Conversion to Aniseed format of data from Yutaka Satous gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).
Original annotation :
Gene name :en.
Expression pattern : nerve cord.
Comment : two pairs of cells in the brain and in the nerve cord.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in green.
Original annotation:
Ci-En is expressed in part of the posterior sensory vesicle, visceral ganglion and neck. The expression pattern of Ci-En has dramatically altered by this stage. Ci-En is expressed in three domains aligned along A-P axis. Interestingly, none of these domains overlaps with the Otx domain.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in green, Pax2/5/8a in red.
Original annotation:
Ci-En is expressed in part of the posterior sensory vesicle, visceral ganglion and neck. The expression pattern of Ci-En has dramatically altered by this stage. Ci-En is expressed in three domains aligned along A-P axis. The first and second domains of Ci-En correspond to the anterior and posterior parts of the Ci-Pax2/5/8a domain.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in green, Hox1 in red.
Original annotation:
Ci-En is expressed in part of the posterior sensory vesicle, visceral ganglion and neck. The second and third domains of Ci-En overlap with the anterior and posterior parts of the first domain of Ci-Hox1.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in green, Fgf8/17/18 in red.
Original annotation:
Ci-En is expressed in neck and in part of posterior sensory vesicle. Expression of Ci-Fgf8/17/18 was detected in a small region of the CNS, which overlaps with posterior part of the third domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in red, SoxB1 in red.
Original annotation:
Ci-En is expressed in part of the posterior sensory vesicle, visceral ganglion and neck. The first domain of Ci-SoxB1 is immediately posterior to the second domain of Ci-En and the second domain of Ci-SoxB1 overlaps with the anterior part of the third domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the mid tailbud stage. En expression is shown in green, Dmbx in red.
Original annotation:
Ci-En is expressed in posterior sensory vesicle, visceral ganglion and neck. The Ci-Dmbx domain was located between the second and third domains of Ci-En at this stage.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Mid tailbud stage wild type embryo probed for en expression. No picture available in the article.
Original Annotation :
En expression was detected in A12.240, A13.474, A13.473 and A12.239 cell pairs (visceral ganglion).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors.
Original comment: : In control Ciona embryos, En (green) is normally expressed in A11.120 and A11.119 (arrows), whereas Vsx is expressed in A11.117 (arrowhead). The gap between En and Vsx expression represents A11.118(unfilled arrowhead).
Second image is the magnification of the boxed zone.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Vsx is expressed in A11.1117 motor neuron precursor.
Original comment: : In control Ciona embryos, En (green) is normally expressed in A11.120 and A11.119 (arrows), whereas Vsx is expressed in A11.117 (arrowhead). The gap between En and Vsx expression represents A11.118(unfilled arrowhead).
Second image is the magnification of the boxed zone.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Engrailed expression is lost.
Original comment: : Treatment with U0126 at 6 hpf results in loss of En and ectopic Vsx in all four descendants of A9.30 (arrowheads).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Vsx is expressed in A11.117, A11.118, A11.120 and A11.119 motor neuron precursors.
Original comment: : Treatment with U0126 at 6 hpf results in loss of En and ectopic Vsx in all four descendants of A9.30 (arrowheads).
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors.
Original comment: : Treatment with U0126 at 8 hpf does
not abolish En (arrows), but ectopic Vsx is still seen in A11.118 (arrowheads).
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Vsx is expressed in A11.117 and A11.118 motor neuron precursors.
Original comment: : Treatment with U0126 at 8 hpf does
not abolish En (arrows), but ectopic Vsx is still seen in A11.118 (arrowheads).
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors (arrows).
Original comment: : Treatment with U0126 at 9 hpf does not alter En or Vsx expression.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Vsx is expressed in A11.117 motor neuron precursor (arrowhead).
Original comment: : Treatment with U0126 at 9 hpf does not alter En or Vsx expression.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Late tailbud embryo probed for Ci-Otx expression (in blue), Ci-Eph3 expression (in green) and Ci-En expression (in red).
Original annotation:
According to the gene expression map (Ikuta and Saiga, 2007), expression of ci-En was observed in A13.473, A13.474, A12.238, A11.61 and A11.62 cell pairs.
Aniseed annotation:
These cells have several fate corresponding to lateral visceral ganglion, neck and ventral posterior sensory vesicle.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal cells - neck - |
Late tailbud embryo probed for Ci-Otx expression (in blue), Ci-Eph3 expression (in green) and Ci-En expression (in red) following the injection of Ci-Hox1-MO.
Original annotation:
Expression of the genes that share the expression domain with the Hox genes at tailbud or larval stage was unaffected in the tailbud embryos or larvae injected with MOs against Ci-Hox1, 2, 3, 4 and 5.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal cells - neck - |
Late tailbud embryo probed for Ci-vAChTP expression (in blue), Ci-Pax2/5/8 expression (in green) and Ci-En expression (in red). <
Original annotation:
According to the gene expression map (Ikuta and Saiga, 2007), expression of Ci-En was observed in A13.473, A13.474, A12.238, A11.61 and A11.62 cell pairs.
Aniseed annotation:
These cells have several fate corresponding to lateral visceral ganglion, neck and ventral posterior sensory vesicle.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal cells - neck - |
Late tailbud embryo probed for Ci-vAChTP expression (in blue), Ci-Pax2/5/8 expression (in green) and Ci-En expression (in red) following the injection of Ci-Hox1-MO.
Original annotation:
Expression of the genes that share the expression domain with the Hox genes at tailbud or larval stage was unaffected in the tailbud embryos or larvae injected with MOs against Ci-Hox1, 2, 3, 4 and 5.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal cells - neck - |
Dorsal views of late tailbud stage embryo expressing En. The upper panel shows WISH signals, the middle DAPI signals and the lower the merged images.
Arrowheads indicate nuclei of the cells that express En. Open arrows indicate the constrictions in the row of nuclei of the lateral cells. Red dots indicate nuclei of the landmark cells.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | lateral VG ependymal cells - neck - |
Two-color-fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, Otx in red.
Original annotation:
Ci-En is expressed in the visceral ganglion and in the posterior sensory vesicle. Expression of Ci-En was detected in three domains. The first domain is located immediately posterior to the Ci-Otx.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, Fgf8/17/18 in red.
Original annotation:
Ci-En is expressed in the posterior sensory vesicle and in the visceral ganglion. Expression of Ci-En was detected in three domains. The posterior-most part of the first domainof Ci-Fgf8/17/18 expression domain do not overlap with the third domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stagz. En expression is shown in green, Hox1 in red.
Original annotation:
Ci-En is expressed in the posterior sensory vesicle and in the visceral ganglion. Expression of Ci-En was detected in three domains. The posterior-most part of the first domainof Ci-Hox1 expression domain do not overlap with the third domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, Dmbx in green.
Original annotation:
Ci-En is expressed in the posterior sensory vesicle and in part of the visceral ganglion. Ci-Dmbx is expressed between the second and the third domains of Ci-En without any gap or overlapping.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, SoxB1 in red.
Original annotation:
Ci-En is expressed in the posterior sensory vesicle, the neck and in part of the visceral ganglion. At this stage, the expression of Ci-SoxB1 was detected in four domains in the posterior trunk CNS. The first and third domains coincide with the first and third domains of Ci-En, respectively and the second domain overlaps with the posterior-most part of the second domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color-fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, Hox3 in red.
Original annotation:
Ci-En is expressed in the posterior sensory vesicle, neck and visceral ganglion. The first domain of Ci-Hox3 overlaps with the anterior half of the second domain of Ci-En.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Two-color fluorescence WISH was performed at the late tailbud stage. En expression is shown in green, Pax2/5/8a in red.
Original annotation:
Ci-En is expressed in the visceral ganglion and the posterior sensory vesicle. Expression of Ci-En was detected in three domains. The first and second domains overlap with the anterior and posterior part of the Ci-Pax2/5/8a expression domain.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - posterior sensory vesicle - visceral ganglion - |
Lateral view of the motor ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs.
Engrailed regularory element drives YFP expression in the neck, in A11.119 and in A11.120.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
Lateral view of the motor ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs.
Vsx regularory element drives mCherry expression in A11.117.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Engrailed regularory element drives YFP expression in the neck.
Original comment: Co-electroporation of FGF8/17/18>dnFGFR results in loss of En reporter and expansion of Vsx reporter. Note En expression in the neck, which lies outside the A9.30 lineage.
Nuclei of the A9.30 lineage (A11.120, A11.119, A11.118 and A11.117) are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Vsx regularory element drives mCherry expression in A11.120, A11.119, A11.118 and A11.117.
Original comment: Co-electroporation of FGF8/17/18>dnFGFR results in loss of En reporter and expansion of Vsx reporter. Note En expression in the neck, which lies outside the A9.30 lineage.
Nuclei of the A9.30 lineage (A11.120, A11.119, A11.118 and A11.117) are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>Eph3ΔC overexpression construct.
Engrailed regulatory element drives YFP expression in the A9.30 lineage.
Original comment: Co-electroporation FGF8/17/18>Eph3ΔC results in expansion of En and loss of Vsx reporters.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>Eph3ΔC overexpression construct.
Vsx regulatory element drives no YFP expression.
Original comment: Co-electroporation FGF8/17/18>Eph3ΔC results in expansion of En and loss of Vsx reporters.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | No expression - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>EphrinAb overexpression construct.
Engrailed regulatory element drives YFP expression in the neck, in A11.119 and A11.120.
Original comment: Overexpression of EphrinAb results in Vsx expression in A11.118, but not A11.117, indicating a fate ‘swap’ between these two cells (n=52/100)
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>EphrinAb overexpression construct.
Vsx regulatory element drives mCherry expression in A11.118.
Original comment: Overexpression of EphrinAb results in Vsx expression in A11.118, but not A11.117, indicating a fate ‘swap’ between these two cells (n=52/100)
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caFGFR overexpression construct.
Original comment: Overexpression of a constitutively active form of FGFR (caFGFR) has no effect on En enhancer activity.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caFGFR overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Original comment: Wild-type Vsx expression is seen in 33/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Original comment: Vsx expression is abolished in 44/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | No expression - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Original comment: Wild-type Vsx expression is seen in 33/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Original comment: Vsx expression is abolished in 44/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | No expression - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Original comment: Wild-type Vsx expression is seen in 33/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Original comment: Vsx expression is abolished in 44/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C11.689 (ALX4; VSX1; VSX2) |
| Stained region(s) | No expression - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - |
No data shown in the article for this developmental stage.
En reporter construct is active in A11.20, A11.119 and in the neck at the late tailbud stage.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>mCherry (red nuclei) and En>unc76:YFP(Venus) (green) reporter constructs, FGF8/17/18>Eph3ΔC overexpression construct and treated with the MEK inhibitor U0126 from the early neurula stage.
Original annotation: Treatment of FGF8/17/18>Eph3ΔC-electroporated with U0126 at 6 hpf (20°C) mimics the phenotype of U0126 treatment alone: En reporter is abolished and four A11.117-like cells are specified (n=40/50). This suggests that Ephrin signaling operates upstream of and antagonistic to MAPK in cell fate choice.
Nuclei of the A9.30 lineage are visualized with mCherry (red) driven by the FGF8/17/18 driver.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | No expression - |
Late tailbud embryo probed for Ci-Dmbx expression (in blue), Ci-En expression (in green) and Ci-SoxB1 expression (in red).
Original annotation:
According to the gene expression map (Ikuta and Saiga, 2007), expression of Ci-En was observed in A13.245, A13.242, A13.241, A12.240, A13.476 and A13.475 cell pairs.
Aniseed annotation:
These cells have several fates corresponding to lateral visceral ganglion, neck and ventral posterior sensory vesicle.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
Late tailbud embryo probed for Ci-Dmbx expression (in blue), Ci-En expression (in green) and Ci-SoxB1 expression (in red) following the injection of Ci-Hox3-MO.
Original annotation:
Expression of the genes that share the expression domain with the Hox genes at tailbud or larval stage was unaffected in the tailbud embryos or larvae injected with MOs against Ci-Hox1, 2, 3, 4 and 5.
| Stained molecule | KH2012:KH.C7.431 (EN1; EN2; VAX1) |
| Stained region(s) | neck - visceral ganglion - |
