Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : No distinct zygotic signal was detected

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : nervous system.

Aniseed annotation :
Later expression is detected in a part of the visceral ganglion, suggesting the two prominent cells are precursors of this later expression territory. The anterior expression in is the central a-line neural plate.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis

Lateral view of the motor ganglion of a WT Ciona robusta early tailbud embryo probed for Nkx6 expression (red) and electroporated with Fgf8/17/18>LacZ reporter construct (green nuclei).
Nkx6 is expressed in A11.117, A11.118 and A11.119 motor neuron precursors (arrowheads).
Original comment: Nuclei of the A9.30 lineage are visualized by immunofluorescence detection of nuclear β-galactosidase reporter (green) driven by the FGF8/17/18 driver (cf diagram).

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : nervous system, nerve cord.
Comment : cells near the ganglion and a few cells in the brain

Lateral view of an early tailbud wild type embryo probed for Nk6 expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Nk6 was expressed in the visceral ganglion (A11.119, A11.118 and A11.117 cell pairs) and in the lateral nerve cord (A11.115 cell pair).

Lateral view of the motor ganglion of a WT Ciona robusta early tailbud embryo probed for Nkx6 expression (green) and electroporated with FGF8/17/18>mCherry reporter construct (red).
Nkx6 is expressed in A11.119 (arrowhead), A11.118 and A11.119 motor neuron precursors.
The A9.30 lineage is visualized using the FGF8/17/18 reporter construct (red nuclei and diagram).

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :NK6.
Expression pattern : nervous system, nerve cord.
Comment : cells near the ganglion and a few cells in the brain

Mid tailbud wild type embryo probed for Nk6 expression. No picture available in the article.

Original Annotation :
Nk6 was expressed in the visceral ganglion : A11.118, A12.239, A13.473 cell pairs.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Epidermis

Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo electroporated with Nk6>unc76::GFP (green) reporter construct.
Nk6 enhancer drives GFP expression in A11.118 motor neuron precursor.
Original annotation: Nk6>GFP reporter construct labels frondose motor endplates in 50%of electroporated embryos. (A') Magnified view of boxed area in A