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EST count

27 results

Library Name Percentage Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal 0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library 0 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult 0 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo 0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg 0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva 3 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo 2 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library 0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library 0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis 0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library 0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva 0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells 0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle 0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo 1 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula 0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad 0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex 0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart 0 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland 0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal 0 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal 0 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal 2 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal 0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile 0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) 0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) 5 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

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Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Read more…

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform Ion Torrent PGM
Layout Single-end
Stranded Yes
Read length 20-365
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)
Mutant
Deregulated molecule(s) KH2012:KH.C8.890 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

Read more…

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform Illumina HiSeq 2500
Layout Single-end
Stranded Yes
Read length 101
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 1
Deregulated molecule(s) KH2012:KH.C4.125 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 2
Deregulated molecule(s) KH2012:KH.C2.573 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

53 results

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : Unlocalized signal was detected

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)

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Vegetal view of a WT Ciona robusta late 32-cell stage embryo probed for FoxB expression.
No expression is observed.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Read more…

Original annotation :
Gene name :FoxB.
Expression pattern : A7.4, A7.6, A7.8

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A7.4 cell pair - A7.6 cell pair - A7.8 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64-cell stage embryo probed for FoxB expression following egg micro-injection of a FoxD-a/b MO. FoxB is down-regulated in A-line nerve cord cells and TLCs.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Read more…

Vegetal view of a 64-cell stage embryo probed for FoxB expression following egg micro-injection of a FoxA-a MO. FoxB was down-regulated in A-line nerve cord cells and TLCs.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : A7.4, A7.6, A7.8

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A7.4 cell pair - A7.6 cell pair - A7.8 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Read more…

Vegetal view of a 64 cell stage embryo probed for Ci-FoxB expression.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A7.4 cell pair - A7.6 cell pair - A7.8 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a 64 cell stage embryo probed for Ci-FoxB expression following egg micro-injection of a FGF9 MO. Ci-FoxB was ectopically expressed in the primary notochord lineage, in the neural lineage and in the anterior endoderm.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) a7.10 cell pair - a7.13 cell pair - a7.14 cell pair - A7.1 cell pair - A7.2 cell pair - A7.3 cell pair - A7.4 cell pair - A7.5 cell pair - A7.6 cell pair - A7.7 cell pair - A7.8 cell pair - a7.9 cell pair - b7.10 cell pair - B7.1 cell pair - B7.2 cell pair - B7.5 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

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Vegetal view of a WT Ciona robusta 64-cell stage embryo probed for FoxB expression.
Expression is observed in A7.4, A7.6, A7.8 and B7.4 blastomere pairs.
In schematic representation of gene expression, nerve cord precursors are shown in blue. Blastomeres with gene expression are marked by dots.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A7.4 cell pair - A7.6 cell pair - A7.8 cell pair - B7.4 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Read more…

Original annotation :
Gene name :FoxB.
Expression pattern : A7.6, A8.7, A8.8, A8.15, A8.16

Aniseed annotation :
according to the picture, it seems that there is an expression in B8.8 and B8.7 cell pairs.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - B8.7 cell pair - B8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a 110-cells stage embryo probed for FoxB expression following egg micro-injection of a FGF9/16/20 MO. FoxB is ectopically expressed in A-line notochord.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.13 cell pair - A8.14 cell pair - A8.15 cell pair - A8.16 cell pair - A8.5 cell pair - A8.6 cell pair - A8.7 cell pair - A8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : A7.6, A8.7, A8.8, A8.15, A8.16

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

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Vegetal view of a WT Ciona robusta 112-cell stage embryo probed for FoxB expression.
Expression is observed in A8.7, A8.8, A8.15, A8.16, A7.6, B8.7 and B8.8 blastomere pairs.
In schematic representation of gene expression, nerve cord precursors are shown in blue. Blastomeres with gene expression are marked by dots.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A8.11 cell pair - A8.12 cell pair - A8.15 cell pair - A8.16 cell pair - A8.7 cell pair - A8.8 cell pair - B8.7 cell pair - B8.8 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

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Mid gastrula stage wild type embryo probed for FoxB expression. No picture available in the article.

Original Annotation :
FoxB was expressed in A9.14 cell pair.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A9.14 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Read more…

Vegetal view of a mid gastrula stage wild type embryo probed for FoxB expression.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A9.14 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Read more…

Vegetal view of a mid gastrula stage wild type embryo probed for Ci-FoxB expression.

Original Annotation : Ci-FoxB expression was detected in A9.14 cell pair.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A9.14 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Read more…

Vegetal view of a mid gastrula stage embryo probed for Ci-FoxB expression following the injection of Delta2-MO.
Graph is showing the percentage of embryo with ectopic, strong or weak Ci-FoxB expression.'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression, there is also a important part of 'ectopic' expression.

Original Annotation : following the inhibition of Delta2/notch signaling (due to Delta2-MO) Ci-FoxB was also expressed ectopically in A9.16 cell pair (arrowheads).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A9.14 cell pair - A9.16 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Read more…

Vegetal view of a mid gastrula stage embryo probed for Ci-FoxB expression following the injection of DAPT.
Graph shows the percentage of embryos with ectopic, strong or weak Ci-FoxB expression.'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression, and there is also an important part of 'ectopic' expression.

Original Annotation : following the inhibition of Delta2/notch signaling (due to DAPT) Ci-FoxB was also expressed ectopically in A9.16 cell pair (arrows).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A9.14 cell pair - A9.16 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Read more…

Vegetal view of a mid gastrula stage embryo probed for Ci-FoxB expression following the electroporation of the promoter FOG::Delta2.
Graph showing the percentage of embryos with ectopic, strong or weak Ci-FoxB expression.'Weak' means at least one cell with downregulated expression, 'strong' means at least one A-line precursor cell with strong expression.

Original Annotation : following the overexpression of Delta2/Notch signaling (due to the construct), Ci-FoxB expression was lost.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : nervous system.
Comment : A9.14.

Aniseed annotation:
A staining has been detected part of lateral tail epidermis.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) lateral posterior tail epidermis - row II neural plate -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Read more…

Dorsal and lateral view of a late gastrula stage wild type embryo probed for Ci-FoxB expression. These pictures show expression only in the median neural plate, more precisely in the A10.27-28 cells.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A10.27 cell pair - A10.28 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Dorsal and lateral views of a late gastrula stage embryo, showing expression of Ci-FoxB. Expression of Ci-FoxB was detected by whole-mount in situ hybridization.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A10.27 cell pair - A10.28 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Dorsal view of a mid gastrula stage wild tye embryo probed for Ci-Six3/6 and Ci-FoxB expression. This two expressions are clearly separated by a transverse row of cells.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) A10.27 cell pair - A10.28 cell pair - A10.31 cell pair - A10.32 cell pair - row IV neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : nervous system, epidermis.
Comment : brain, epidermis in the lateral region of the body.

Aniseed annotation:
A staining has been detected in part of the lateral epidermis (head and tail) and in part of row II neural plate.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - row II neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

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Dorsal view of a mid neurula stage wild type embryo probed for Ci-FoxB expression. Expression appeared in two lateral bands in the presumptive posterior sensory vesicle.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - row II neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Dorsal and lateral views of a mid neurula stage embryo, showing expression of Ci-FoxB. Expression of Ci-FoxB was detected by whole-mount in situ hybridization.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - row II neural plate -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) central nervous system - dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : nervous system, epidermis.
Comment : brain, epidermis in the lateral region of the body.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) anterior sensory vesicle - dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal and lateral view of early tailbud stage wild type embryos probed for Ci-FoxB expression. Expression appeared in two lateral bands in the presumptive posterior sensory vesicle.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of an early tailbud stage wild type embryo probed for FoxB expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
FoxB was expressed in A11.63 and A11.64 cell pairs (posterior sensory vesicle).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of an early tailbud stage embryo probed for FoxB expression following the injection of FGF8/17/18 MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Ectopic expression was observed in the neck (A11.61 and A11.62 cell pairs)(black arrow)

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) neck - posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of an early tailbud embryo probed for FoxB expression following the injection of FoxB MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Expression in the posterior sensory vesicle is stronger than in the control (A11.63 and A11.64 cell pairs)(black arrow).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of an early tailbud stage embryo probed for FoxB expression following the injection of Otx MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
FoxB expression was lost in the posterior sensory vesicle (white arrow).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Views of early tailbud stage embryos showing reporter expression of the region upstream of FoxB in mesenchyme, muscle and a part of brain.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) neck - posterior sensory vesicle - tail muscles - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of a WT Ciona robusta early tailbud embryo probed for FoxB (KH.C4.341) expression.
Expression is observed in the lateral epidermis and in the brain.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - neck - posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of a Ciona robusta early tailbud embryo probed for FoxB (KH.C4.341) expression and injected with morpholinos against Admp.
Expression is detected in lateral and ventral epidermis and in the brain.
Original annotation : in Admp morphants, FoxB expression,which marks the lateral epidermal region,was expanded into the ventral region.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - ventral head epidermis - ventral lateral anterior tail epidermis - ventral lateral posterior tail epidermis - ventral midline posterior tail epidermis - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of a Ciona robusta early tailbud embryo probed for FoxB (KH.C4.341) expression and injected with morpholinos against Bmp2/4.
Expression, similar to WT expression, is observed in the lateral epidermis and in the brain.
Original annotation : In Bmp2/4 morphants, we could not observe distinct change of FoxB expression.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

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Lateral view of a Ciona robusta early tailbud embryo probed for FoxB (KH.C4.341) expression and injected with morpholinos against Pinhead (KH.C2.1069).
Weak expression is observed in the lateral epidermis and in the brain.
Original annotation : In Pinhead morphants, FoxB expression was reduced

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) central nervous system - dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :FoxB.
Expression pattern : nervous system, epidermis.
Comment : brain, epidermis in the lateral region of the body.

Aniseed annotation:
Regarding the picture, we can't be more accurate.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) epidermis - nervous system -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Dorsal and lateral views of mid tailbud embryos showing sensory vesicle expression and lateral epidermal expression in the head and tail.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Mid tailbud stage wild type embryo probed for FoxB expression. No picture available in the article.

Original Annotation :
FoxB was expressed in A12.238 cell pair (part of visceral ganglion).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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The central nervous system of a mid tailbud stage wild type embryo co-electroporated with FGF8/17/18>RFP (red) and FoxB>GFP (green).

Original Annotation :
Ci-FoxB was expressed in A11.64 and A11.63 cell pairs (posterior sensory vesicle), in A11.62 and A11.61 cell pairs (neck) and in A11.120, A11.119, A11.118 and A11.117 cell pairs (visceral ganglion).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) neck - posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Views of mid tailbud stage embryos showing reporter expression of the region upstream of FoxB in mesenchyme and notochord.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) notochord -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Views of mid tailbud stage embryos showing reporter expression of the region upstream of FoxB in epidermis.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) central nervous system - dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Lateral view of a late tailbud stage wild type embryo probed for Ci-Six3/6 and Ci-FoxB expression. Expressions are clearly separated by a transverse row of cells. Ci-Six3/6: brown and Ci-foxB: yellow

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) anterior sensory vesicle - neurohypophysis primordium - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Late tailbud stage wild type embryo probed for Ci-FoxB expression. Expression was observed just posterior to the otolih domain, more in the caudal nerve cord and in the epidermis.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) anterior sensory vesicle - dorso lateral head epidermis - lateral anterior tail epidermis - lateral posterior tail epidermis - tail nerve cord -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Views of larvae showing reporter expression of the region upstream of FoxB in mesenchyme and notochord.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) notochord -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Views of larvae showing reporter expression of the region upstream of FoxB in epidermis.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) epidermis -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Views of larvae showing reporter expression of the region upstream of FoxB in mesenchyme, muscle and a part of brain.

Stained molecule KH2012:KH.C4.341 (FOXA2; FOXB1; FOXB2)
Stained region(s) anterior sensory vesicle - neck - posterior sensory vesicle - tail muscles - visceral ganglion -