Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : Unlocalized signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : No distinct zygotic signal was detected.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : nervous system.
Comment : Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Ventral and dorsal view of a late neurula stage wild type embryo probed for Ci-POU-IV expression showing one (first picture) and two pairs (second picture) of labeled epidermal cells. The two pairs of epidermal cells in the posterior dorsal part of the embryo could be the precursors of the tail epidermal sensory neurons.

Original annotation : dorsal view of an early tailbud stage wild type embryo probed for Ci-POU4 expression. Expression was detected in a bilateral pair of cells anterior to the neural plate (arrow).

Aniseed annotation : according to the picture, staining might have been detected in the rostral trunk epidermal neurons.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : nervous system.
Comment : Almost all neurons; Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Dorsal and lateral views of an early tailbud stage embryo, showing expression of ci-PouIV. Expression of ci-PouIV was detected by whole-mount in situ hybridization.

Original annotation : Lateral, frontal and dorsal views of an early tailbud stage wild type embryo probed for Ci-POU-IV expression showing expression in two bilaterally symmetrical pairs of dorsal ectodermal cells, one just above the neural tube, and the second one more posterior. In the tail, labeled epidermal cells are increasing in number.

Original annotation : frontal and Lateral views of early tailbud stage wild type embryo probed for Ci-POU-IV expression. Expression expands to some ventral epidermal cells of the tail, whose number gradually increases during development. In the dorsal part of the embryo, transcript are present in bilaterally symmetrical pairs of cells at the borders of the rolling neural plate.

Aniseed annotation : is the expression in the precursors of the ventral caudal epidermal neurone (CEN) or in the ventral midline posterior tail epidermis?

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :POU4.
Expression pattern : nervous system.
Comment : Almost all neurons; Maternal signal were observed ubiquitously mainly in the early claeving stages, but also somewhat in the later stage.

Lateral view of a mid tailbud stage wild type embryo probed for Ci-POU4 expression.

Original annotation : in addition to expression in anterior cells, expression was also activated in additional anterior epidermal cells adjacent to the palps and by isolated cells of the dorsal and ventral midline epidermis of the trunk and tail.

Original annotation : lateral, dorsal caudal and enlarged view of the trunk of a mid tailbud wild type embryo probed for Ci-POU-IV expression. Signal is found in the ATEN and RTEN precursors, as well as in CEN. Ci-POU-IV is also expressed in the CNS, in two cells that will contribute to form the posterior sensory vesicle of the larva CNS.

Lateral view of an embryo probed for ci-Pou4 expression injected with the control LacZ MO.
Original annotation: Pou4 marks the ESNs in control embryos injected with the control LacZ MO.

Dorsal and lateral views of a late tailbud stage embryo, showing expression of ci-PouIV. Expression of ci-PouIV was detected by whole-mount in situ hybridization.

No data shown in the article for this developmental stage.
PouIV is expressed in A12.239 at the late tailbud stage.

Original annotation : lateral and dorsal view of a tadpole stage wild type embryo probed for Ci-POU-IV expression. The first picture shows expression in the palp cells, RTEN, ATEN, CEN as well as in the most posterior part of the sensory vesicle (arrow). A weakly labeled motoneuron is also visible in the viseral ganglion (arrowhed). The third picture shows expression in an ATEN (arrowhead) and in two symmetrical motoneurons of the visceral ganglion (arrows). The last picture shows expression in three RTEN chains (arrowheads), as well as in the posteroir sensory vesicle (arrow).

Left side view of a young adult stage wild type embryo probed for Ci-POU4 expression. Expression was detected in the region of the endostyle.