Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Fertilized eggs just after the second phase of ooplasmic segregation viewed from lateral side. Localization or distribution pattern of Ci-Tolloid maternal transcript : Pem localized signal was detected.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : Pem-like localized signal was detected

Localization or distribution pattern of Ci-Tolloid (which is a maternal transcript) in 8-cell stage embryos, revealed by whole-mount in situ hybridization. Embryos viewed from the right side and from the posterior side. Pem localized signal was detected in B4.1 cell pair.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : No distinct zygotic signal was detected.
Comment : The expression in the posterior end/endodermal strand is maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : No distinct zygotic signal was detected.
Comment : The expression in the posterior end/endodermal strand is maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : A7.1, A7.2, A7.5, B7.1, B7.2.
Comment : The expression in the posterior end/endodermal strand is maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : A7.1, A7.2, A7.5, B7.2.
Comment : The expression in the posterior end/endodermal strand is maternal.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : endoderm, unidentifiable.
Comment : The expression in the posterior end/endodermal strand is maternal.

Expression observed in mesenchyme in 0,5% of stained embryo.

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : endoderm, unidentifiable.
Comment : The expression in the posterior end/endodermal strand is maternal.

Dorsal and lateral views of an early tailbud stage embryo, showing expression of Ci-Tolloid. Expression of Ci-Tolloid was revealed by whole-mount in situ hydridization.

Early-tailbud embryos electroporated with Mesp > lacZ, detected by immunofluorescence (red), and hybridized with Tolloid antisense RNA probes (green).

Original annotation:
They observed conspicuous TVC specific expression of GATAa and Tolloid, while NK4 and Bmp2/4 expression could not be detected above background levels.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : mesenchyme, nervous system, nerve cord.
Comment : mesenchyme, trunk ventral cells. The expression in the posterior end/endodermal strand is maternal.

Lateral view of a tailbud stage embryo probed for Tolloid expression. Original Annotation : Tolloid expression was detected in mesenchyme, nervous system, nerve cord. Comment : mesenchyme, trunk ventral cells. The expression in the posterior end/endodermal strand is maternal. Aniseed annotation : a staining has been detected in the nervous system but also in the tail nerve cord and the palps. A staining has also been detected in the mesenchyme.

Mid-tailbud embryo electroporated with Mesp > lacZ (red) and hybridized with a Tolloid antisense RNA probe (green).

Original annotation :
Tolloid shows expression in TVCs, notochord, palp region and parts of the central nervous system. Note Bmp2/4 and Tolloid expression in both the leader (L) and trailer (T) TVCs (arrowheads), but not in the ATMs (arrows).

Aniseed Annotation :
Expression in the central nervous system and in the notochord is very weak.

Aniseed Comment : update of annotation of (Imai et al.,2004), based on (Imai et al.,2006).

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :Tolloid.
Expression pattern : mesenchyme, notochord, nervous system, nerve cord.
Comment : mesenchyme: trunk ventral cells. The expression in the posterior end/endodermal strand is maternal.

Dorsal and lateral views of a late tailbud stage embryo, showing expression of Ci-Tolloid. Expression of Ci-Tolloid was revealed by whole-mount in situ hydridization.