Aniseed - Gene Card

EST count

27 results

Library Name	Percentage	Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal		0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library		0 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult		0 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo		0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg		0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva		0 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo		0 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library		0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library		0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis		0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library		0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva		0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells		0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle		0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo		0 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula		0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad		0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex		0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart		0 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland		0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal		0 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal		0 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal		0 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal		0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile		0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx)		0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)		0 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

No result

In Situ Experiment Data

30 results

Click here to visualize with MorphoNet

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of a mid tailbud stage wild type embryo probed for Chox10 expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Expression of Chox10 in the visceral ganglion is localized in A11.117 cell pair.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors.
Original comment: : In control Ciona embryos, En (green) is normally expressed in A11.120 and A11.119 (arrows), whereas Vsx is expressed in A11.117 (arrowhead). The gap between En and Vsx expression represents A11.118(unfilled arrowhead).
Second image is the magnification of the boxed zone.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	visceral ganglion -

Dorsal view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Vsx is expressed in A11.1117 motor neuron precursor.
Original comment: : In control Ciona embryos, En (green) is normally expressed in A11.120 and A11.119 (arrows), whereas Vsx is expressed in A11.117 (arrowhead). The gap between En and Vsx expression represents A11.118(unfilled arrowhead).
Second image is the magnification of the boxed zone.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Engrailed expression is lost.
Original comment: : Treatment with U0126 at 6 hpf results in loss of En and ectopic Vsx in all four descendants of A9.30 (arrowheads).

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	No expression -

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Vsx is expressed in A11.117, A11.118, A11.120 and A11.119 motor neuron precursors.
Original comment: : Treatment with U0126 at 6 hpf results in loss of En and ectopic Vsx in all four descendants of A9.30 (arrowheads).

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors.
Original comment: : Treatment with U0126 at 8 hpf does not abolish En (arrows), but ectopic Vsx is still seen in A11.118 (arrowheads).

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	visceral ganglion -

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Vsx is expressed in A11.117 and A11.118 motor neuron precursors.
Original comment: : Treatment with U0126 at 8 hpf does not abolish En (arrows), but ectopic Vsx is still seen in A11.118 (arrowheads).

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Engrailed is expressed in A11.120 and A11.119 motor neuron precursors (arrows).
Original comment: : Treatment with U0126 at 9 hpf does not alter En or Vsx expression.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	visceral ganglion -

Dorsal view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Engrailed (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Vsx is expressed in A11.117 motor neuron precursor (arrowhead).
Original comment: : Treatment with U0126 at 9 hpf does not alter En or Vsx expression.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Mnx is expressed in A11.118 and A10.57.
Original comment: :Mnx is normally expressed in A11.118 (double arrowhead) and in a motoneuron from the A9.29 lineage (A10.57, asterisk).
Second image is the magnification of the boxed zone.

Stained molecule	KH2012:KH.L128.12 (HOXA3; HOXB3; MNX1)
Stained region(s)	visceral ganglion -

Lateral view of the motor ganglion of a WT Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with DMSO from 6hpf (control).
Vsx is expressed in A11.117 (arrow).

Second image is the magnification of the boxed zone.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Mnx is expressed in A10.57, A10.58, A10.64 and A19.63.
Original comment: : U0126 at 6 hpf results in loss of Mnx from A11.118 and expansion of Mnx in the A9.29 lineage (asterisks), and ectopic Vsx in all four descendants of A9.30 (arrowheads).

Stained molecule	KH2012:KH.L128.12 (HOXA3; HOXB3; MNX1)
Stained region(s)	visceral ganglion -

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 6hpf.
Vsx is expressed in A11.117, A11.118, A11.119 and A11.120.
Original comment: : U0126 at 6 hpf results in loss of Mnx from A11.118 and expansion of Mnx in the A9.29 lineage (asterisks), and ectopic Vsx in all four descendants of A9.30 (arrowheads).

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Mnx is expressed in A10.57 and A10.58.
Original comment: U0126 at 8 hpf results in loss of Mnx from A11.118 and expansion of Mnx in the A9.29 lineage (asterisks), and ectopic Vsx in A11.118 (arrowheads).

Stained molecule	KH2012:KH.L128.12 (HOXA3; HOXB3; MNX1)
Stained region(s)	visceral ganglion -

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 8hpf.
Vsx is expressed in A11.117 and A11.118.
Original comment: U0126 at 8 hpf results in loss of Mnx from A11.118 and expansion of Mnx in the A9.29 lineage (asterisks), and ectopic Vsx in A11.118 (arrowheads).

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Mnx is expressed in A10.57 and A11.118 (asterik and double arrowhead respectively).
Original comment: U0126 at 9 hpf does not alter Mnx or Vsx expression

Stained molecule	KH2012:KH.L128.12 (HOXA3; HOXB3; MNX1)
Stained region(s)	visceral ganglion -

Lateral view of the motor ganglion of a Ciona robusta mid tailbud embryo probed for Mnx (green) and Vsx (red) expressions and treated with the MEK inhibitor U0126 from 9hpf.
Vsx is expressed in A11.117 (arrowhead).
Original comment: U0126 at 9 hpf does not alter Mnx or Vsx expression

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Lateral view of the head of a WT Ciona robusta late tailbud embryo probed for Vsx (KH.C11.689) expression. All embryos counterstained by fluorescent antibody stain for X-gal (red nuclei), denoting A9.30 lineage labeled by electroporation with Fgf8/17/18>lacZ.
B' is a higher magnification of B.
Original annotation:In situ hybridization of Vsx in green, showing expression in A11.117 and A13.474 (arrowhead and double arrowhead in B', respectively)

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with Vsx>unc76::GFP (green) and Fgf8/17/18>unc76::mCherry (red) reporter constructs.
Original annotation:A9.30 lineage is labeled by electroporation of Fgf8/17/18>mCherry.
Colocalization of GFP and mCherry is indicated in yellow and corresponds to Vsx>GFP activity in A11.117. Vsx>GFP is not seen in A13.474, as this cell has not yet been born.
A fusion of a DNA sequence spanning the entire Vsx transcribed region with unc-76-tagged enhanced green fluorescent protein (GFP) recapitulated expression in A11.117 and A13.474.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C5.5 (FGF17; FGF18; FGF8)
Stained region(s)	lateral VG ependymal cells - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Lateral view of the motor ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs.
Engrailed regularory element drives YFP expression in the neck, in A11.119 and in A11.120.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck - visceral ganglion -

Lateral view of the motor ganglion of a WT Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs.
Vsx regularory element drives mCherry expression in A11.117.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Engrailed regularory element drives YFP expression in the neck.
Original comment: Co-electroporation of FGF8/17/18>dnFGFR results in loss of En reporter and expansion of Vsx reporter. Note En expression in the neck, which lies outside the A9.30 lineage.
Nuclei of the A9.30 lineage (A11.120, A11.119, A11.118 and A11.117) are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>dnFGFR overexpression construct.
Vsx regularory element drives mCherry expression in A11.120, A11.119, A11.118 and A11.117.
Original comment: Co-electroporation of FGF8/17/18>dnFGFR results in loss of En reporter and expansion of Vsx reporter. Note En expression in the neck, which lies outside the A9.30 lineage.
Nuclei of the A9.30 lineage (A11.120, A11.119, A11.118 and A11.117) are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>Eph3ΔC overexpression construct.
Engrailed regulatory element drives YFP expression in the A9.30 lineage.
Original comment: Co-electroporation FGF8/17/18>Eph3ΔC results in expansion of En and loss of Vsx reporters.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	visceral ganglion -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>Eph3ΔC overexpression construct.
Vsx regulatory element drives no YFP expression.
Original comment: Co-electroporation FGF8/17/18>Eph3ΔC results in expansion of En and loss of Vsx reporters.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>EphrinAb overexpression construct.
Engrailed regulatory element drives YFP expression in the neck, in A11.119 and A11.120.
Original comment: Overexpression of EphrinAb results in Vsx expression in A11.118, but not A11.117, indicating a fate ‘swap’ between these two cells (n=52/100)
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck - visceral ganglion -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>EphrinAb overexpression construct.
Vsx regulatory element drives mCherry expression in A11.118.
Original comment: Overexpression of EphrinAb results in Vsx expression in A11.118, but not A11.117, indicating a fate ‘swap’ between these two cells (n=52/100)
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.
Dashed line indicates midline.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caFGFR overexpression construct.
Original comment: Overexpression of a constitutively active form of FGFR (caFGFR) has no effect on En enhancer activity.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck - visceral ganglion -

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caFGFR overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.117.
Original comment: Wild-type Vsx expression is seen in 33/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	No expression -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	No expression -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
Vsx regularory element drives mCherry expression in A11.119, A11.118 and A11.117 in 23/100 tested embryos.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) causes ectopic Vsx expression in only 23/100 embryos.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	visceral ganglion -

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	No expression -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Lateral view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

Dorsal view of the motor ganglion of a Ciona robusta late tailbud embryo co-electroporated with FGF8/17/18>H2B:CFP (blue nuclei), En>unc76:YFP(Venus) (green) and Vsx>unc76:mCherry (red) reporter constructs and Fgf8/17/18>caEph3 overexpression construct.
En regularory element drives YFP expression only in the neck.
Original comment: Constitutive Ephrin signaling by overexpression of self-dimerizing, constitutively active Eph3(caEph3) abolishes En reporter expression.
Nuclei of the A9.30 lineage are visualized with CFP (blue) driven by the FGF8/17/18 driver.

Stained molecule	KH2012:KH.C7.431 (EN1; EN2; VAX1)
Stained region(s)	neck -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

Lateral view of the visceral ganglion of a WT Ciona robusta late tailbud stage embryo probed for Vsx expression (green) and electroporated with Fgf8/17/18>nlsLacZ (labels in red nuclei from A9.30 lineage) and COE>mCherry (no staining, control experiment).
Vsx is expressed in A13.474 (motor ganglion Interneuron 1 precursor, open arrowhead) and A11.117 (motor ganglion Interneuron 2 precursor, closed arrowhead).

Stained molecule	KH2012:KH.C11.689 (ALX4; VSX1; VSX2)
Stained region(s)	lateral VG ependymal cells -