Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Very weak signals were detected. Gene: Ci-ZF143. Expression pattern: expressed and unlocalized.

Embryo electroporated with a construct that contains the Ci-fog cis-regulatory region from -2000bp to +1bp, included 12 GATA sites (construct named G12-bpbra::NLS-lacZ in the paper). Staining is observed in a- and b-line at the 8-cell stage.

Representative embryos electroporated with a construct that contains ci-fog regulatory region from -2000pb to +1pb (construct named pfog2000 in the paper). No expression observed at the 8-cell stage.

Embryo electroporated with a construct that contains Ci-fog regulatory region from -2000bp to +1bp (construct named pfog2000 in the paper). Staining is observed in a-line and b-line.

8-cell stage embryo electroporated with a construct that contains ci-fog regulatory region from -314pb to +1pb (construct named pfog-314::NLS-lacZ in the paper).

Animal, lateral and vegetal views of a 16-cell stage embryo, showing expression of ci-fog in the animal region.

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: The signal did not seem to be zygotic. Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Embryo electroporated with a construct that contains the Ci-fog cis-regulatory region from -2000bp to +1bp, included 12 GATA sites (construct named G12-bpbra::NLS-lacZ in the paper). Staining is observed in a- and b-line at the 16-cell stage.

Embryo electroporated with a construct that contains the Ci-fog cis-regulatory region from -2000bp to +1bp, included 12 GATA sites (construct named G12-bpbra::NLS-lacZ in the paper). Staining is observed in a- and b-line at the 16-cell stage.

Representative embryos electroporated with a construct that contains ci-fog regulatory region from -2000pb to +1pb (construct named pfog2000 in the paper). No expression observed at the 16-cell stage.

Embryo electroporated with a construct that contains ci-fog cis-regulatory region from -2000bp to +1bp (construct named pfog2000 in the paper). Staining is observed in the a-line and b-line.

16-cell stage embryo electroporated with a construct that contains ci-fog regulatory region from -314pb to +1pb (construct named pfog-314::NLS-lacZ in the paper).

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: The signal did not seem to be zygotic. Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Representative embryos electroporated with a construct that contains ci-fog regulatory region from -2000pb to +1pb (construct named pfog2000 in the paper). Staining is observed in a-line and b-line at the late 32-cell stage.

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: The signal did not seem to be zygotic. Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Representative embryos electroporated with a construct that contains ci-fog regulatory region from -2000pb to +1pb (construct named pfog2000 in the paper). Staining is observed in a-line and b-line at the 64-cell stage.

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Lateral view (picture 1) and representative embryos electroporated with a construct that contains ci-fog regulatory region from -2000pb to +1pb (construct named pfog2000 in the paper). Staining is observed in a-line and b-line at the 110-cell stage.

Embryos electroporated with a construct that contains the Ci-fog cis-regulatory region from -2000bp to +1bp, included 12 GATA sites (construct named G12-bpbra::NLS-lacZ in the paper). Staining is observed in the a and b-line at the 110-cell stage.

Embryos electroporated with a construct that contains the Ci-fog cis-regulatory region from -290bp to +1bp (construct named pfog-290 in the paper). No expression is observed at the 110-cell stage. No picture available in the paper.

Representative embryos at 110-cell stage co-injected with a construct that contains ci-fog regulatory region from -314pb to +1pb (construct named pfog-314::NLS-lacZ in the paper) and control-MO.

Embryos electroporated with a construct that contains ci-fog regulatory region from -354pb to +1pb (construct named pfog-354 in the paper).
Level of lacZ reporter activity for pfog-354 construct: 50-90% of animal cells stained. No picture available in the paper.

Embryos electroporated with a construct that contains ci-fog regulatory region from -530pb to +1pb (construct named pfog-530 in the paper).
More than a half of embryos counted have all animal cells stained. No picture available in the paper.

Level of LacZ reporter activity in a 110-cell stage embryo. This construct contains ci-fog regulatory region from -314pb to +1pb (construct named pfog-m314 in the paper) and has a point mutation in each of the two GATA binding sites. No picture available in the paper.

Embryos electroporated with a construct that contains ci-fog regulatory region from -556pb to +1pb (construct named pfog-556 in the paper).
The level of lacZ reporter activity is indicated for pfog-556 construct: 50-90% of animal cells stained. No picture available in the paper.

Embryo electroporated with a construct that contains the Ci-fog cis-regulatory region from -215bp to +1bp (construct named pfog-215 in the paper). No expression is observed at the 110-cell stage. No picture available in the paper.

Embryos electroporated with a construct that contains ci-fog regulatory region from -300pb (construct named pfog-300-bpbra in the paper).

Representative embryos electroporated with a construct (dINT construct in the paper) containing the entire Ci-fog cis regulatory region from -314bp and lacking of the first intron. This construct maintains animal expression.

Expression of ZNF266 at the neural plate stage. Graphs show the percentage of embryos with strong, weak or ectopic expression, as indicated in the key. Embryos were scored 'ectopic' if at least one additional cell showed expression. The number of embryos analysed is indicated above the graphs. cont, control.

Vegetative view of an early gastrula. ZnF266 is expressed in A9.13 and A9.15 blastomere pair (medial row I neural plate cells), as well as in in A9.14 and A9.16 blastomere pair (medial row II neural plate cells)

Vegetative view of an early gastrula. ZnF266 is expressed in A9.13 and A9.15 blastomere pair (medial row I neural plate cells), as well as in in A9.14 and A9.16 blastomere pair (medial row II neural plate cells)

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Lateral view with anterior to the left of an early neurula stage embryo electroporated stained with X-Gal and electroporated with p12xTCF::LacZ (a Wnt canonical pathway reporter construct).
Original annotation: Expression was observed in the precursors of the posterior ventral midline epidermal cells (black arrowheads).

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: expression pattern: Muscle (secondary lineage) Muscle (primary lineage)

Lateral view with anterior to the left of an initial neurula stage embryo electroporated stained with X-Gal and electroporated with p12xTCF::LacZ (a Wnt canonical pathway reporter construct).
Original annotation: Expression was observed in the precursors of the posterior ventral midline epidermal cells.

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: expression pattern: Muscle (secondary lineage) Muscle (primary lineage)

Lateral view with anterior to the left of an early tailbud stage embryo electroporated stained with X-Gal and electroporated with p12xTCF::LacZ (a Wnt canonical pathway reporter construct).
Original annotation: Expression was observed in the posterior ventral midline epidermal cells (black arrowheads).

Lateral view with anterior to the left of a mid tailbud stage embryo electroporated stained with X-Gal and electroporated with p12xTCF::LacZ (a Wnt canonical pathway reporter construct).
Original annotation: Expression was observed in the posterior ventral midline epidermal cells (black arrowheads).

Conversion to Aniseed format of data from Miwata et al. (2006) Dev Biol. 292(2)
Original annotation: Gene: Ci-ZF143. Expression pattern: no distinct zygotic signal was detected.

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: expression pattern: Muscle (secondary lineage) Muscle (primary lineage)

Conversion to Aniseed format of Ghost database entry from Ogasawara et al., Dev Genes Evol. 2002; 212:173-185. Original annotation: . Expression pattern: Pharyngeal gill

A young adult probed for hemocyte type-2 gene for cluster 03463.

Original Annotation : Expression of type-2 gene was detected in hemocytes in pharyngeal gill but also in stomach and intestine.