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EST count

27 results

Library Name Percentage Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal 0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library 2 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult 0 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo 0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg 0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva 21 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo 16 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library 0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library 0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis 0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library 0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva 0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells 2 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle 1 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo 0 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula 0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad 0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex 0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart 5 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland 0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal 0 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal 0 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal 4 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal 0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile 0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) 0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) 3 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

Read more…

Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Read more…

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform Ion Torrent PGM
Layout Single-end
Stranded Yes
Read length 20-365
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)
Mutant
Deregulated molecule(s) KH2012:KH.C8.890 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

Read more…

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform Illumina HiSeq 2500
Layout Single-end
Stranded Yes
Read length 101
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 1
Deregulated molecule(s) KH2012:KH.C4.125 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 2
Deregulated molecule(s) KH2012:KH.C2.573 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

49 results

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : No signal was detected

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : No distinct zygotic signal was detected

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : muscle.
Comment : the expression in muscle cells was very weak

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a WT Ciona robusta neurula embryo probed for Meis expression and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers, and in tail muscles
Original annotation: Meis is expressed in column 1 of neural plate row III daughters, with stronger expression in the posterior cell a10.73

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression and injected with morpholino oligos against Nodal.
Meis expression is observed in a10.73, a10.74 (as in the WT) and in a10.66 and a10.67 blastomers from neural plate rows IIIa and IIIb, and in tail muscles.
Original annotation: In Nodal-inhibited embryos, Meis was ectopically expressed in column 2 (red arrowheads) of most embryos (88%) and in column 3 in a proportion of embryos (18%).
n=total number of embryos analysed.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, treated with a pharmacological inhibitor of TGFβ type I receptors ALK4, ALK5 and ALK6 (SB431542) that inhibits Nodal activity and counterstained with DAPI.
Meis expression is observed in a10.74, a10.73 (as in the WT) and in a10.65 and a10.66 blastomers from neural plate rows IIIa and IIIb, and in tail muscles.
Original annotation: In Nodal-inhibited embryos, Meis was ectopically expressed in column 2 (red arrowheads) of most embryos (96%) and in column 3 in a proportion of embryos (27%).
n=total number of embryos analysed.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression and electroporated with pFOG>Nodal to drive expression of Nodal throughout the animal hemisphere from the 16-cell stage of development.
Meis expression is observed in tail muscles and is lost in a-neural plate.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a WT Ciona robusta neurula embryo probed for Meis expression.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, but also in tail muscles.
Original annotation: Meis is expressed in column 1 of neural plate row III daughters, with stronger expression in the posterior cell a10.73.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, treated from the 76-cell stage with DAPT, an inhibitor of γ-secretase, an enzyme required for Notch receptor processing and counterstained with DAPI.
Meis expression is observed in a10.74, a10.73 (as in the WT) and in a10.65 and a10.66 blastomers from neural plate rows IIIa and IIIb, and in tail muscles.
Original annotation: this treatment resulted in ectopic expression of Meis in column 2 (red arrowheads).
n=total number of embryos analysed.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, injected with mRNA encoding a dominant negative form of Suppressor of Hairless, a transcription factor known to mediate Notch signalling and counterstained with DAPI.
Meis expression is observed in a10.74, a10.73 (as in the WT), a10.65 and a10.66 blastomers from neural plate rows IIIa and IIIb, and in tail muscles.
Original annotation: This treatment resulted in ectopic expression of Meis in column 2 (red arrowheads).
n=total number of embryos analysed.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression and electroporated with pFOG>Delta-like to drive expression of Delta-like throughout the animal hemisphere from the 16-cell stage of development
Meis expression is observed in the tail muscles, but is lost in a-neural plate.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a WT Ciona robusta neurula embryo probed for Meis expression.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1, and in tail muscles.
Original annotation: Meis is expressed in column 1, with stronger expression in the posterior cell a10.73.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression and injected with a morpholino against Snail.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 3 and in tail muscles. Level and profile of expression are similar to the WT.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73 cell pair - a10.74 cell pair - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression and electroporated with pETR>Snail to overexpress Snail throughout the neural plate.
Meis expression is observed in a10.74 and a10.73 blastomers from one side only and in tail muscles.
Original annotation: Overexpression of Snail resulted in downregulation of Meis.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73* - a10.74* - tail muscles -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a WT Ciona robusta neurula embryo probed for Meis expression and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.73 cell pair - a10.74 cell pair -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, injected with morpholinos against Snail and Su(H)DBM (dominant negative form of Suppressor of Hairless), and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1 (blue arrowhead) and ectopic expression is observed in a10.66 and a10.65 from column 2 (green arrowhead).
This expression pattern is observed in most of the double morphant embryos.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair -

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, injected with morpholinos against Snail and Su(H)DBM (dominant negative form of Suppressor of Hairless), and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1 (blue arrowhead) and ectopic expression is observed in a10.66 and a10.65 from column 2 (green arrowhead) and a10.98 and a10.97 from column 3 (pink arrowhead).
This expression pattern is observed in a minority of the double morphant embryos.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - a10.97 cell pair - a10.98 cell pair - row III-A neural plate - row III-B neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Read more…

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, injected with morpholinos against Snail and Su(H)DBM (dominant negative form of Suppressor of Hairless), and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1 (blue arrowhead) and ectopic expression is observed in a10.66 and a10.65 from column 2 (green arrowhead).
This expression pattern is observed in most of the double morphant embryos.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair -

Vegetal view of a Ciona robusta neurula embryo probed for Meis expression, injected with morpholinos against Snail and Su(H)DBM (dominant negative form of Suppressor of Hairless), and counterstained with DAPI.
Meis expression is observed in a10.74 and a10.73 blastomers from neural plate rows IIIa and IIIb, column 1 (blue arrowhead) and ectopic expression is observed in a10.66 and a10.65 from column 2 (green arrowhead) and a10.98 and a10.97 from column 3 (pink arrowhead).
This expression pattern is observed in a minority of the double morphant embryos.
Second image is a HD picture of the same embryo.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) a10.65 cell pair - a10.66 cell pair - a10.73 cell pair - a10.74 cell pair - a10.97 cell pair - a10.98 cell pair - row III-A neural plate - row III-B neural plate -

C. robusta formely Ciona int. type A, Stage 16 (late neurula)

Read more…

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - tail muscles -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : muscle, nervous system, epidermis.
Comment : the expression in muscle cells was very weak

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - tail epidermis - tail muscles -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - tail muscles -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of an early tailbud stage wild type embryo probed for Meis expression.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) dorso lateral anterior tail epidermis - dorso lateral head epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Dorsal view of a tailbud stage embryo probed for Meis expression following egg micro-injection of a DMRT1 MO. Meis is down-regulated in a-line neural cells.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Dorsal view of a tailbud stage embryo probed for Meis expression following egg micro-injection of a Otx MO. Meis is down-regulated in a-line neural cells.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Vegetal view of a early tailbud stage embryo probed for Meis expression following egg micro-injection of a ZicL MO. Meis is down-regulated in a-line neural cells.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) tail epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : nervous system, nerve cord, epidermis, unidentifiable.
Comment : epidermis in the posterior half of the trunk and in the anterior half of the tail

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) dorso lateral anterior tail epidermis - dorso lateral head epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Lateral and dorsal views of an early tailbud wild type embryos probed for Ci-Meis expression. These pictures show expression in a small ventromedial cell cluster of the sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) head epidermis -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Early tailbud wild type embryos probed for Ci-meis expression. Expression was restricted in a ventromedial domain of the sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :meis.
Expression pattern : nervous system, nerve cord, epidermis, unidentifiable.
Comment : epidermis in the posterior half of the trunk and in the anterior half of the tail.

Aniseed annotation :
The comments in Imai et al., Science 2006 were taken into account.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) dorso lateral anterior tail epidermis - dorso lateral head epidermis - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral and dorsal views of a mid tailbud stage embryo, showing expression of Ci-Meis.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - epidermis -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of mid tailbud wild type embryos probed for Ci-meis expression.

Original Annotation : Expression was detected in the sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - tail muscles -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Read more…

Dorsal view of a late tailbud stage wild type embryo probed for Ci-Meis expression. Expression was detected in the anterior ventral sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) head epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

Read more…

Lateral view of a late tailbud stage wild type embryo probed for Ci-Meis expression. This picture shows expression in a small ventromedial cell cluster of the sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Late tailbud stage embryos, showing expression of Ci-Meis in the dorso lateral head epidermis and in the anterior sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - epidermis -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Conversion to aniseed format of DBTGR database entry from Kusakabe team.

Original annotation:
Brain vesicle; muscle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - posterior sensory vesicle - tail muscles -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Conversion to aniseed format of DBTGR database entry from Kusakabe team.

Original annotation:
Mesenchyme; epidermis; muscle; papilla.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) epidermis - tail muscles -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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Original annotation :Lateral and dorsal views respectively of tailbud embryos electroporated with Meis_ciCNE1. In (C) reporter expression (arrow) is localised to the sensory vesicle, adjacent to the otolith pigment cell. In (D) reporter expression (arrow) is confined to the lateral tail epidermis of the right hand side.
This reporter construct drove expression into the ventral sensory vesicle (first picture) and anterior tail epidermis (second picture), in a pattern similar to the endogenous expression pattern of the Ciona Meis gene.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - lateral anterior tail epidermis -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

Read more…

This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

Read more…

This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

Read more…

This reporter construct shows no activity in Ciona intestinalis late tailbuds.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

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Original annotation : Dorso-lateral view of the head of a tailbud embryo electroporated with Meis_ciCNE10. Reporter expression (arrow) is localised to the sensory vesicle, adjacent to the otolith pigment cell (ot).

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Late tailbud stage embryos, showing expression of Ci-Meis in the dorso lateral head epidermis and in the anterior sensory vesicle.

Stained molecule KH2012:KH.C10.174 (MEIS1; MEIS2; MEIS3)
Stained region(s) anterior sensory vesicle - head epidermis -