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EST count

27 results

Library Name Percentage Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal 0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library 3 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult 2 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo 0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg 0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva 13 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo 21 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library 0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library 0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis 0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library 0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva 0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells 21 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle 0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo 0 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula 0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad 0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex 0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart 1 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland 1 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal 3 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal 7 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal 7 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal 2 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile 1 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx) 0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) 0 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

   Expression profile summary from all experiments in WT and mutant conditions:
Relative Log Expression RLE

Be careful, RLE and 2RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

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Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform Illumina HiSeq 2000
Layout Paired-end
Stranded Yes
Read length 49
Normalized data types
  • FPKM
  • RLE
Wild type - Replica 1
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)
Wild type - Replica 2
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Read more…

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform Ion Torrent PGM
Layout Single-end
Stranded Yes
Read length 20-365
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)
Mutant
Deregulated molecule(s) KH2012:KH.C8.890 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
C. robusta formely Ciona int. type A, Stage 8 (64-cell)
C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

Read more…

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform Illumina HiSeq 2500
Layout Single-end
Stranded Yes
Read length 101
Normalized data types
  • RPKM
Wild type
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 1
Deregulated molecule(s) KH2012:KH.C4.125 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)
Mutant 2
Deregulated molecule(s) KH2012:KH.C2.573 -
Territories whole embryo -
Biomaterial(s) C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

46 results

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

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Dorsal view of a mid neurula embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

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Dorsal view of a mid neurula embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

Read more…

Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: expression pattern: Mesenchyme Trunk lateral cells

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 17 (initial tailbud I)

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Dorsal view of an initial tailbud embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904.

Original annotation: expression pattern: Mesenchyme Trunk lateral cells

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Even if cleavage is arrested, gene expression profile corresponds to the observed stage.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Embryos developed from eggs injected with the Ci-Twist-like1a/b morpholino.

Original annotation: the expression was completely suppressed.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Expression pattern of Ci-Tram1/2 gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Embryos developed from eggs injected with with Ci-Twist-like1a mRNA.

Original annotation: microinjection of synthetic Ci-Twist-like1a mRNA resulted in the ectopic expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

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Lateral view of an early tailbud embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Even if cleavage is arrested, gene expression profile corresponds to the observed stage.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Even if cleavage is arrested, gene expression profile corresponds to the observed stage.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Embryos developped from eggs injected with Ci-FGF9/16/20 morpholino.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Read more…

Expression pattern of Ci-AKR1a gene in embryos whose cell division was arrested during the period from the 110-cell stage to the early tailbud stage. Embryos developped from eggs injected with Ci-FGF9/16/20 mRNA.

Original annotation: the gene seems to be expressed in most B-line, but not in A-line blastomeres.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) endodermal strand - posterior ventral head endoderm - primary muscle lineage (anterior) - trunk ventral cells (B7.5 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Mesenchyme Trunk lateral cells

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage wild type embryo. This picture shows expression in mesenchyme cells and TLCs.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud wild type stage embryo probed for Ci-AKR1a expression.

Ci-AKR1a was expressed in mesenchyme.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Mid tailbud embryos probed for Ci-AKR1a expression following the injection of Ci-Rga-MO and Ci-Rga mRNA lacking the target site of the morpholino.

Original Annotation : The expression of markers for the mesenchyme was detected in all cases, showing that defects caused by Ci-Rga knock-down were attenuated by Ci-RgamMO mRNA overexpression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Mid tailbud embryos probed for Ci-AKR1a expression following the injection of Ci-Rga-MO.

Original Annotation : The expression of a mesenchyme marker, Ci-AKR1a, was lost in 10 (50%) of 20 knock-down embryos and severely reduced in seven (35%) of them.
First picture : Ci-Rga knock-down embryos with a weaker phenotype that develop into embryos covered by an epidermis layer.
Second picture : Ci-Rga knock-down embryos with severe phenotype that develop into cell aggregates.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Expression of Ci-AKR1a gene observed at the mid tailbud stage. Embryos developed from eggs injected with Ci-Twist-like1a mRNA.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) whole embryo -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Expression of Ci-AKR1a gene observed at the mid tailbud stage. Embryos developed from eggs injected with the Ci-Twist-like1a/b morpholino.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud embryo probed for Ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Expression of Ci-AKR1a gene observed at the mid tailbud stage. Embryos developped from eggs injected with Ci-FGF9/16/20 morpholino.

Aniseed annotation: according to the picture, expression in the mesenchyme and in the trunk lateral cells is not very clear.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Expression of Ci-Tram1/2 gene observed at the mid tailbud stage. Embryos developped from eggs injected with Ci-FGF9/16/20 mRNA.

Aniseed annotation: according to the picture, expression in the mesenchyme and in the trunk lateral cells is not very clear.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with Ci-talin (notochord) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cieg010p13 is classified as " a/p axis" phenotype: embryos were extended along the anterior-posterior (a/p) axis, but did not form normal tadpoles, with head and tail being indistinguishable.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) notochord -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with ci-AKR1a expression.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation Embryos injected with MO against cilv006h21 as classified as " ball" phenotype: they developped into spherical-shaped cell masses, like balls. In these embryos, detection of the tissue specific markers indicated thaht almost the major tissues were lost or reduced.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cieg028g04 as classified as " irregular" phenotype: embryos formed an irregular cellular mass. The a/p axis and trunk and tail could not be distinguished.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cieg009p15 as classified as " irregular" phenotype: embryos formed an irregular cellular mass. The a/p axis and trunk and tail could not be distinguished.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cieg039g16 is classified as " a/p axis" phenotype: embryos were extended along the anterior-posterior (a/p) axis, but did not form normal tadpoles, with head and tail being indistinguishable.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cicl017h21 is classified as " a/p axis" phenotype: embryos were extended along the anterior-posterior (a/p) axis, but did not form normal tadpoles, with head and tail being indistinguishable.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against cieg010p13 is classified as " a/p axis" phenotype: embryos were extended along the anterior-posterior (a/p) axis, but did not form normal tadpoles, with head and tail being indistinguishable.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of a mid tailbud stage embryo probed with Ci-AKR1a (mesenchyme) expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage.

Original annotation: Embryos injected with MO against ciad033i21 is classified as " flat" phenotype: eggs develop into flattened larvae and they lost the expression of markers for endoderm, notochord and mesenchyme.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo, showing expression of Ci-AKR1 in mesenchyme.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Read more…

Lateral view of mid tailbud stage embryo, injected with Ci-PGAP1 MO. Effects of functional suppression of Ci-PGAP1 on differenciation of mesenchyme (assessed by expression of Ci-AkR1).

Original annotation:
Differentiation of mesenchyme was not completely inhibited in Ci-PGAP1 knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of mid tailbud stage embryo, injected with Ci-ZF278 MO. Effects of functional suppression of Ci-ZF278 on differenciation of mesenchyme (assessed by expression of Ci-AkR1).

Original annotation:
Differentiation of mesenchyme was not completely inhibited in Ci-ZF278 knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of mid tailbud stage embryo, injected with Ci-spatial/C4orf17 MO. Effects of functional suppression of Ci-spatial/C4orf17 on differenciation of mesenchyme (assessed by expression of Ci-AKR1).

Original annotation:
Differentiation of mesenchyme was blocked in Ci-spatial/C4orf17 knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of mid tailbud stage embryo, injected with Ci-FLJ10634 MO. Effects of functional suppression of Ci-FLJ10634 on differenciation of mesenchyme (assessed by expression of Ci-AKR1).

Original annotation:
Differentiation of mesenchyme was not completely inhibited in Ci-FLJ10634 knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of mid tailbud stage embryo, injected with Ci-beta-catenin MO. Effects of functional suppression of Ci-beta-catenin on differenciation of mesenchyme (assessed by expression of Ci-AKR1).

Original annotation:
Ci-AKR1a was not detected in Ci-beta-catenin knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of mid tailbud stage embryo, injected with Ci-C10orf11 MO. Effects of functional suppression of Ci-C10orf11 on differenciation of mesenchyme (assessed by expression of Ci-AkR1).

Original annotation:
Differentiation of mesenchyme was not completely inhibited in Ci-C10orf11 knock-down embryos.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

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Lateral view of a mid tailbud stage embryo probed with AKR1a expression. Expression of tissue-specific differenciation markers in morpholino oligonucleotide-injected embryos at the stage corresponding to the mid tailbud stage. Embryos injected with MO against cieg007f18.

Original annotation: in the cieg007f18 knockdown embryos, whole-mount in situ hybridization showed that mesenchyme was lost.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Conversion to Aniseed format of Ghost database entry from Satou Y et al., Development 2001; 128:2893-2904. Original annotation: . Expression pattern: Mesenchyme Trunk lateral cells

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) trunk lateral cells (A7.6 line) -

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Dorsal view of a late tailbud stage wild type embryo probed for ciad005j06. This picture shows expression in mesenchyme cells.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 23 (late tailbud I)

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Late tailbud stage embryo probed for ciad005j06 expression following egg micro-injection of a cieg003h01-MO.

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

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Conversion to Aniseed format of Ghost database entry from Kusakabe T et al., Dev Biol. 2002; 242:188-203. Original annotation: . Expression pattern: Mesenchyme

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s)

C. robusta formely Ciona int. type A, Stage 48 (young adult)

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Conversion to Aniseed format of Ghost database entry from Ogasawara et al., Dev Genes Evol. 2002; 212:173-185. Original annotation: . Expression pattern: not detected

Stained molecule KH2012:KH.C1.222 (AKR1C1; AKR1C2; AKR1C4)
Stained region(s) No expression -